Relevance of markers as to prediabetic status of 100 runs in a random forest analysis.

<p>Relevance of markers as to prediabetic status of 100 runs in a random forest analysis.</p

Logistic regression as to risk for prediabetes of SNPs per allele (2a) or sum score (2b).

<p>Logistic regression as to risk for prediabetes of SNPs per allele (2a) or sum score (2b).</p

Number of total homozygous or heterozygous risk alleles.

<p>Values are given as mean ±1 standard deviation.</p

Baseline characteristics of normoglycemic versus prediabetic participants.

<p>Values are given as mean ±1 standard deviation or as absolute or relative frequencies.</p>*<p>males / ^#females;</p>§<p>t-test,</p>†<p>chi-square test;</p>‡<p>estimated by the Friedewald formula.</p

Ocular Ultrasound of a normal eye.

<p>The ultrasound probe is applied to the closed eye and transmission gel used to avoid any pressure on the eye. The normal eye (here right eye) appears as a round hypoechoic (grey-black) structure, and the cornea (2) is a thin hyperechoic (white) layer next to the eyelid (1). This is adjacent to the anterior chamber (hypoechoic water-filled cavity). Below the chamber, a hyperechoic iris and ciliary bodies (4) follow with the anterior (hyperechogenic) reflection of the lens (3). The lens itself is hypoechogenic with a smaller reflection on the rear side (5). The vitreous body is hypoechogenic due to its water-filled cavity. Retina may not be differentiated from choroidal layers, while the optic nerve appears as a hypoechoic linear structure (7) entering the vitreous chamber (6).</p

Ocular ultrasound examination of a vitreous haemorrhage (VH) in the left eye.

<p>In this case Terson's syndrome appeared as a hyperechogenic membrane caused by clotted blood within the vitreous body. According to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114907#pone-0114907-g001" target="_blank">Fig. 1</a> iris and ciliary bodies (4) as well as the anterior (3) and posterior reflection (5) of the lens and the optic nerve (7) are visible.</p

Learning curve of sensitivity, specificity, positive and negative predictive value and accuracy of investigator I and II.

<p>Pts.  =  patients.</p><p>Learning curve of sensitivity, specificity, positive and negative predictive value and accuracy of investigator I and II.</p

Influence of Ocln on epidermal differentiation and EMT markers, cell-cell adhesion and paracellular Ca²⁺ permeability.

<p>(A) (left side) Western Blot analysis of 3D skin models after silencing of Ocln with 2 different siRNAs shows downregulation of Ocln and of the epidermal differentiation marker involucrin and an upregulation of TG1. There is no alteration for the EMT markers E-cadherin and vimentin. Same amounts of protein were loaded and actin or tubulin were used as gel loading controls. A representative experiment is shown (n = 3). (right side) Semiquantitative analysis of Ocln, involucrin, TG1, E-cadherin and vimentin. Band intensities were normalized to actin (Inv, TG1) or tubulin (E-cad, vim). Subsequently, the values were normalized to control siRNA treated cells (n = 3; mean±SEM; *p<0.05, ***p<0.001 compared to control siRNA). (B) Calcium induced cell-cell adhesion was investigated with a hanging drop assay at the indicated time points. Significantly greater numbers of particles (indicating less cell-cell adhesion) were found in the suspensions of Ocln knock-down cells compared to the controls (*: <i>p</i><0.05 <i>n</i> = 3), (C) Electrophysiological studies of paracellular Ca²⁺-permeability in Ocln siRNA-treated cultured keratinocytes revealed an increase in paracellular permeability for Ca²⁺ in Ocln knock-down cells compared to cells treated with control siRNA (n = 6, mean ± SEM). (D) Example for the knock-down of Ocln in siRNA treated submerged cells. Mean knock-down of Ocln in the cells used for experiments in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055116#pone-0055116-g005" target="_blank">Figure 5</a> B, C and 6 was 76% +/−9%.</p

Protein expression and localization of TJ proteins in the various skin tumors.

<p>Loss: complete loss of expression, altered: altered localization. % in “loss of expression” denotes the percentage of all tumors, % in “altered localization” denotes the percentage of positive tumors.</p>1<p>downregulation in the uppermost layers.</p>2<p>downregulation in the lowermost layers.</p>3<p>broader expression.</p><p>Abbreviations see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055116#pone-0055116-t001" target="_blank">table 1</a>.</p

Correlation of presence of TJ proteins and tumor grading of SCC.

<p>Pos, positive; neg, negative. Further abbreviations see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055116#pone-0055116-t001" target="_blank">table 1</a>.</p