389 research outputs found
Overeenkomsten en verschillen in de bestuursrechtelijke handhaving bij het bouw- en woningtoezicht van het huidige stelsel in Nederland en Nordrhein-Westfalen
in deze scriptie wordt verslag gedaan van een onderzoek naar de overeenkomsten en verschillen in de bestuursrechtelijke handhaving van het bouw- en woningtoezicht van het huidige stelsel in Nederland en Nordrhein-Westfalen
Human Osteoblast Differentiation and Bone Formation: Growth Factors, Hormones and Regulatory Networks
Osteoporosis is the most common bone disease and is
characterized by low bone mass, micro architectural deterioration and
decreased bone quality resulting in increased risk of fractures.
Osteoblasts, the bone forming cells, play a crucial role in the
regulation of bone mass and bone quality. Osteoblasts are of
mesenchymal origin and undergo a complex differentiation process
regulated by many endocrine and autocrine factors. In order to
develop novel bone anabolic drugs, more knowledge concerning
osteoblast biology is required. In this thesis we investigated the
processes of human osteoblast differentiation and matrix
mineralization. Human osteoblast-based models of bone formation were
used in which the role of glucocorticoids (GCs), 11β-hydroxysteroid
dehydrogenase type 1 (11β-HSD1), the Wnt signaling pathway and the
activin A-follistatin system were studied
Calcifying vascular smooth muscle cells and osteoblasts: Independent cell types exhibiting extracellular matrix and biomineralization-related mimicries
Background: Ectopic vascular calcifications represent a major clinical problem associated with cardiovascular disease and mortality. However, the mechanisms underlying pathological vascular calcifications are largely unknown hampering the development of therapies to tackle this life threatening medical condition. Results: In order to gain insight into the genes and mechanisms driving this pathological calcification process we analyzed the transcriptional profile of calcifying vascular smooth muscle cells (C-VSMCs). These profiles were compared to differentiating osteoblasts, cells that constitute their physiological calcification counterparts in the body. Overall the transcriptional program of C-VSMC and osteoblasts did not overlap. Several genes, some of them relevant for bone formation, were distinctly modulated by C-VSMCs which did not necessarily lose their smooth muscle cell markers while calcifying. Bioinformatics gene clustering and correlation analysis disclosed limited bone-related mechanisms being shared by two cell types. Extracellular matrix (ECM) and biomineralization genes represented common denominators between pathological vascular and physiological bone calcifications. These genes constitute the strongest link between these cells and represent potential drivers for their shared end-point phenotype. Conclusions: The analyses support the hypothesis that VSMC trans-differentiate into C-VSMCs keeping their own identity while using mechanisms that osteoblasts use to mineralize. The data provide novel insights into groups of genes and biological processes shared in MSC and VSMC osteogenic differentiation. The distinct gene regulation between C-VSMC and osteoblasts might hold clues to find cell-specific pathway modulations, opening the possibility to tackle undesired vascular calcifications without disturbing physiologic bone formation and vice versa
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