Bioefficiency of Chromolaena odorata (Linn.) on hematological and lipid profiles in sublethal cyanide poisoning in male wistar rats

Chromolaena odorata is an invasive plant which is acclaimed to have cyanide remediation potential from contaminated sites. This examination means to decide the impact of ethanol concentrates of C. odorata (ECO), sodium thiosulphate and a mix of both on hematological parameters and blood lipid profile of rodents presented to potassium cyanide. (KCN). A sum of thirty five male Wistar rats partitioned into seven groups of five units were used. KCN Group rats were administered with KCN alone. Rats in 100ECO, 150ECO, 200ECO groups were administered with 100, 150 and 200 mg/kg body weight of ECO respectively. Rats under Na2sS2O3 and Na2S2O3+ECO groups were administered 200 mg/kg sodium thiosulphate and sodium thiosulphate with ECO at 200 mg/kg respectively. The trial was done in about a month. Toward the finish of the investigation, the packed cell volume (PCV), hemoglobin level (Hb), Red blood cells (RBC) and white blood cells (WBC) were resolved utilizing known biochemical methodology. The outcomes demonstrated a noteworthy increment (p ? 0.05) in PCV, Hb, RBC and WBC level of remedial groups when contrasted with the cyanide group. Total cholesterol (TC), and triglyceride (TG) were altogether lower while HDL-cholesterol was fundamentally expanded in all the treated groups when contrasted and the untreated group given cyanide alone. However, no significant difference in LDL-cholesterol was indicated in all therapeutic groups compared with the cyanide group. The study revealed that C. odorata at the tested doses was able to improve the hematological parameters and lipid profile in cyanide exposed rats

Screening for rhodanese producing Bacterium in freshly pressed Cassava effluents of a Cassava processing industry channeled to Odo-Oba Stream in Ogbomoso-Nigeria

Rhodanese is a key enzyme that plays an important role in cyanide detoxification. The enzyme was extracted, purified and physico-chemically characterised from Bacillus licheniformis which demonstrated the highest efficacy compared to the seven isolates of bacteria of the cassava processing industry effluent morpholologically and biochemically characterised.  Statistical analysis was performed using one-way ANOVA and values were considered significant at p<0.05. This study showed that the optimum growth temperature was 350C at a pH 9.0. The highest duration time for the synthesis of rhodanese was at 40 hours. Potassium cyanide (KCN) and casein were the best carbon and nitrogen sources. The enzyme has a specific activity of 10.99 RU/mg, with a purification fold of 4.38, a percentage yield of 15.96%. The apparent Km for KCN and Sodium thiosulphate (Na2S2O3) were determined to be 30.24mM and 24.93mM respectively while their Vmax were 5.40 RU /ml/min and 5.07 RU /ml/min respectively.  The optimum pH and temperature were 8.0 and 50 0C respectively. The enzyme showed a high stability at 500C. The enzyme showed specificity at 6.78 RU/ml/min for Na2S2O3 while it was inhibited by other sulphur containing substrates namely 2-mercaptoethanol, ammonium persulphate, and sodium metabisulphite The enzyme activity was not inhibited by metal ions such as (K+, Mg2+, Ba2+, Ni2+, Sn2+ and Na+) at 1mM and 10mM and was not significant (p>0.05). Therefore, B. licheniformis have the potentials of reducing cyanide pollution thereby enhancing effective management of cassava mill effluent before eventual discharge into the environment and this may be developed into a more effective tool for bioremediation.&nbsp