13 research outputs found

    Convective heat transfer between a moving solid spherical particle and a viscous gas

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    An approximate solution of a boundary value problem for the convective heat transfer equation is obtained by the method of matched asymptotic expansions for small P´eclet and Reynolds numbers. When solving the stationary system of gasdynamic heat transfer equations including the system of Navier-Stokes equations linearized in the velocity, the convective heat transfer equation, and the Poisson equation, it is assumed that the temperature dependences of the viscosity, thermal conductivity, and density of a gaseous medium are power-la

    Diffusiophoresis and photophoresis of heated large nonvolatile aerosol spherical particles

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    This paper describes a theoretical study of the steady motion of a large solid nonvolatile aerosol spherical particle, which contains thermal sources within itself, in a concentration gradient of binary gas mixture components. It is assumed that an average particle surface temperature significantly differs from the temperature of the binary gas mixture surrounding it. Equations of gas dynamics are solved taking into account the power-law dependence of the molecular transfer coefficients (viscosity, thermal conductivity, and diffusion) and the density of the gaseous medium on temperature. Under boundary conditions, diffusion and thermal slip are taken into accoun

    Simulation the process of scientific potential development of the personality at the university: approaches and ideas

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    It has been established the presence of a high percentage of students with a reproductive level of development of the scientific potential of the individual (39%). There have been identified significant deficiencies in the technological readiness of students for research. It is shown the predominance of students with a creative and heuristic level of development of the scientific potential of the personality in the humanities in comparison with students of natural sciences and mathematical specialtie

    Mapping of T7 RNA polymerase active site with novel reagents – oligonucleotides with reactive dialdehyde groups

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    AbstractOligonucleotides of a novel type containing 2′-O-β-ribofuranosyl-cytidine were synthesized and further oxidized to yield T7 consensus promoters with dialdehyde groups. Both types of oligonucleotides were tested as templates, inhibitors, and affinity reagents for T7 RNA polymerase and its mutants. All oligonucleotides tested retained high affinity towards the enzyme. Wild-type T7 RNA polymerase and most of the mutants did not react irreversibly with oxidized oligonucleotides. Affinity labeling was observed only with the promoter-containing dialdehyde group in position (+2) of the coding chain and one of the mutants tested, namely Y639K. These results allowed us to propose the close proximity of residue 639 and the initiation region of the promoter within initiation complex. We suggest the oligonucleotides so modified may be of general value for the study of protein-nucleic acid interactions

    Fluorescent 2-pyrimidinone nucleoside in parallel-stranded DNA

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    Stretches of parallel-stranded (ps) double-helical DNA can arise within antiparallel-stranded (aps) Watson-Crick DNA in looped structures or in the presence of sequence mismatches. Here we studied an effect of a pyrimidinone-G (PG) base pair on the stability and conformation of the ps DNA to explore whether P is useful as a structural probe

    Fluorescent 2-pyrimidinone nucleoside in parallel-stranded DNA

    No full text
    Stretches of parallel-stranded (ps) double-helical DNA can arise within antiparallel-stranded (aps) Watson-Crick DNA in looped structures or in the presence of sequence mismatches. Here we studied an effect of a pyrimidinone-G (PG) base pair on the stability and conformation of the ps DNA to explore whether P is useful as a structural probe
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