63 research outputs found

    Phloretin Affects the Voltage Gating of Alpha-Hemolysin Channel

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    Чиновник или еретик? Проблема ‘профессии’ и жизнетворчество в публицистике Евгения Замятина после 1917 г.

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    The article explores the literary and self-described worldview of Evgenij Zamjatin after the October Revolution. In three ‘autobiographies’ from the 1920s he stressed the crucial role of the 1917 events in his destiny. Post-revolutionary years witnessed an explosion of Zamjatin’s nonfiction, whereby the issue of self-determining in the face of a new Russia was pervasive. His articles and interviews from the 1920s and 1930s, at first sight, give two mutually contradictory definitions of his ‘job’ or, rather, ‘profession’ prior to emigration in 1931: a literary clerk in state service and a fundamentally opposed ‘heretic’. He entered world culture, first of all, as the author of the dystopian novel We and a ‘heretic’. However, his self-identification was far more complex and complicated, a case in point being the fact that in 1934, already apparently an emigrant, he applied for membership in the Union of Soviet Writers. This article tries to solve the contradiction, examining the storyline of an inner conflict – between an actor and a dreamer, a believer and a heretic, an officer of the state and a sceptic – that is recurrent in his nonfiction writing. Moreover, the author suggests that the ambivalence of Zamjatin resists definite evaluation. It is possible to view his ambivalence not only as a psychological or worldview peculiarity but as a manifestation of a ‘life-creation’ strategy.The article explores the literary and self-described worldview of Evgenij Zamjatin after the October Revolution. In three ‘autobiographies’ from the 1920s he stressed the crucial role of the 1917 events in his destiny. Post-revolutionary years witnessed an explosion of Zamjatin’s nonfiction, whereby the issue of self-determining in the face of a new Russia was pervasive. His articles and interviews from the 1920s and 1930s, at first sight, give two mutually contradictory definitions of his ‘job’ or, rather, ‘profession’ prior to emigration in 1931: a literary clerk in state service and a fundamentally opposed ‘heretic’. He entered world culture, first of all, as the author of the dystopian novel We and a ‘heretic’. However, his self-identification was far more complex and complicated, a case in point being the fact that in 1934, already apparently an emigrant, he applied for membership in the Union of Soviet Writers. This article tries to solve the contradiction, examining the storyline of an inner conflict – between an actor and a dreamer, a believer and a heretic, an officer of the state and a sceptic – that is recurrent in his nonfiction writing. Moreover, the author suggests that the ambivalence of Zamjatin resists definite evaluation. It is possible to view his ambivalence not only as a psychological or worldview peculiarity but as a manifestation of a ‘life-creation’ strategy

    Adaptive landscape gardening in a metropolis

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    The article focuses on a solution to the quality-of-life issues, public health by using of new forms of adaptive gardening in the landscape of a large city. Reasoning and calculations of the efficiency for an alternative method of growing medicinal plants were made

    Probing Amphotericin B Single Channel Activity by Membrane Dipole Modifiers

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    The effects of dipole modifiers and their structural analogs on the single channel activity of amphotericin B in sterol-containing planar phosphocholine membranes are studied. It is shown that the addition of phloretin in solutions bathing membranes containing cholesterol or ergosterol decreases the conductance of single amphotericin B channels. Quercetin decreases the channel conductance in cholesterol-containing bilayers while it does not affect the channel conductance in ergosterol-containing membranes. It is demonstrated that the insertion of styryl dyes, such as RH 421, RH 237 or RH 160, in bilayers with either cholesterol or ergosterol leads to the increase of the current amplitude of amphotericin B pores. Introduction of 5α-androstan-3β-ol into a membrane-forming solution increases the amphotericin B channel conductance in a concentration-dependent manner. All the effects are likely to be attributed to the influence of the membrane dipole potential on the conductance of single amphotericin B channels. However, specific interactions of some dipole modifiers with polyene-sterol complexes might also contribute to the activity of single amphotericin B pores. It has been shown that the channel dwell time increases with increasing sterol concentration, and it is higher for cholesterol-containing membranes than for bilayers including ergosterol, 6-ketocholestanol, 7-ketocholestanol or 5α-androstan-3β-ol. These findings suggest that the processes of association/dissociation of channel forming molecules depend on the membrane fluidity

    Methodological provision for the assessment of audit risk during the audit of tax reporting

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    International audienceThe problematics and the goal of the research: The lack of theoretical foundations as well as the practical necessity for organizations to create a methodology for assessing the audit risk in carrying out the audit of tax reporting, determined the research objective: the development of theoretical and practical recommendations regarding the methodological provision for the assessment of audit risks as well as the methodology for establishing the relationship between the adequate tax reporting and the managerial decisions of owners on the basis of tax audit results. Methods used: a method based on the theory of fuzzy sets and the basics of the theory of information asymmetry. Results achieved: the development of a methodology for assessing an audit risk in carrying out the audit of tax reporting and establishment of relationship between the theory of information asymmetry and the effectiveness of the users' managerial decisions. The conclusions of the research: practical implementation of the methodology in organizations with different taxation systems has proved the relationship between the theory of information asymmetry and optimization of the users' managerial decisions. The practical benefits from the obtained results make it possible to increase the efficiency of organizations' activities and to confirm to the tax authorities the timeliness of the calculation and payment of taxes. These methods are the basis for the development of a theory for assessing audit risks in carrying out tax audits Keywords: methodological provision, qualitative assessment, audit risk, tax audit, tax reporting, asymmetric information

    Modeling and control of robot manipulators with the constraints at the moving objects

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    In this paper a method of constructing a model of control over compliant motion of robot manipulators is considered. In this method the elastic deformations of flexible manipulators elements are used as feedback signals. © 2015 IEEE

    Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module

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    To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet–purple protein samples. We characterize the spectroscopic properties of the obtained pigment–protein complexes and the thermodynamics of liposome–protein carotenoid transfer and demonstrate the delivery of carotenoid echinenone from AnaCTDH into liposomes with an efficiency of up to 70 ± 3%. Most importantly, we show efficient carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species (ROS). Incubation of neuroblastoma cell line Tet21N in the presence of 1 μM AnaCTDH binding echinenone decreased antimycin A ROS production by 25% (p < 0.05). The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery.BMBF, 01DJ15007, Carotenoidbindende photoschaltbare Proteine: Lichtinduzierte Dynamik und Anwendungen in modernen mikroskopischen Verfahre
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