Comparison of renal function parameters between WT1 positive and WT1 negative diabetic subjects.

<p>Box plots comparing; A) Estimated GFR; B) Urine protein-to- creatinine ratio; C) Urine albumin-to-creatinine ratio; and D) serum Creatinine levels between WT1 positive and WT1 negative diabetic patients. The boxes indicate median and 25th and 75th percentiles; Outliers are indicated by closed dots. Data were compared by the Mann-Whitney U test. p<0.05 was considered significant.</p

Comparison of WT-1 expression and presence of proteinuria (ACR) in diabetic patients at various eGFR cutoffs.

<p>Bar graph showing percentage of patents detected with proteinuria or WT1 expression in urinary exosomes at various cutoff values of eGFR between 60–90 ml. min⁻¹/1.73 m²). WT-1 expression was detected in higher percentage of patients at earlier fall in GFR (eGFR<70/80/90 ml. min⁻¹/1.73 m²).</p

Detection of WT1 protein in urinary exosomes of diabetic patients with or without proteinuria.

<p>A) Representative immunoblots for WT1 and TSG101 proteins in urinary exosome samples from, type 1 diabetic patients with or without proteinuria and healthy controls. Exosomal protein obtained from same urine volume was loaded for all the samples. B) Frequency of WT1 expression in urinary exosomes from diabetic patients with or without proteinuria and healthy controls. All subjects were positive for TSG101 protein, an exosomal marker (data not shown). Densitometry analysis of WT1 bands in: C) Type-1 diabetic patients, using Mann-Whitney U test, and D) Proteinuria and Non-Proteinuria groups, using ANOVA rank test. The boxes indicate median and 25th and 75th percentiles; Outliers are indicated by closed dots. p<0.05 was considered significant.</p

Supplementary Data File- Hypophosphataemic osteomalacia in a patient with neurofibromatosis type 1- a role for FGF23?

<h2>Supplementary Data File</h2

Sahoo-Supplement Fig 1.Hypophosphataemic osteomalacia in a patient with neurofibromatosis type 1- a role for FGF23?

<b>Family pedigree </b

Sahoo-Supplement Fig 2.pdf

<p><b>Micro-architectural parameters of iliac crest bone evaluated by µCT</b></p> <p>(a) 3-D µCT images showing a normal bone and gross micro-architectural deterioration in the proband’s bone, (b<b>)</b> All parameters of cortical bone microarchitecture; T.Ar (periosteal area), B.Ar (cortical mean cross-section area), Cs.Th (cortical thickness), T.Pm (periosteal perimeter), and B.Pm (cortical bone perimeter) are significantly lower in the proband compared to age and sex-matched healthy control,<b> </b>(c)<b> </b>Parameters of trabecular bone microarchitecture; Tb.Th (trabecular thickness), and Tb.N (trabecular number) are decreased, while Tb.Sp (trabecular separation) and SMI (structure model index) are increased in the proband’s bone. All values are expressed as mean ± S.E. </p> <p>* p<0.05 and ** p<0. 01versus control</p

Supplementary Data-Hypophosphataemic osteomalacia in a patient with neurofibromatosis type 1- a role for FGF23?

Supplementary Dat

Supplement Figures - Hypophosphataemic osteomalacia in a patient with neurofibromatosis type 1- a role for FGF23?

<p><b>Supplement Figure 1</b></p> <p><b><i>Family pedigree</i> </b><br> <b></b></p><p><br></p><p><br></p> <p><b>Supplement Figure 2</b></p> <p><b><i>Micro-architectural parameters of iliac crest bone evaluated by µCT</i></b></p> <p>(a) 3-D µCT images showing a normal bone and gross micro-architectural deterioration in the proband’s bone, (b<b>)</b> All parameters of cortical bone microarchitecture; T.Ar (periosteal area), B.Ar (cortical mean cross-section area), Cs.Th (cortical thickness), T.Pm (periosteal perimeter), and B.Pm (cortical bone perimeter) are significantly lower in the proband compared to age and sex-matched healthy control,<b> </b>(c)<b> </b>Parameters of trabecular bone microarchitecture; Tb.Th (trabecular thickness), and Tb.N (trabecular number) are decreased, while Tb.Sp (trabecular separation) and SMI (structure model index) are increased in the proband’s bone. All values are expressed as mean ± S.E. </p> <p>* p<0.05 and ** p<0. 01 versus control</p

Supplementary Data File- Hypophosphataemic osteomalacia in a patient with neurofibromatosis type 1- a role for FGF23?

Supplement data file-NF1-FGF2