Open Lab Notebooks: An Extreme Open Science Initiative - Aled Edwards Presentation - Scientists and society: striking a new bargain

<p>Aled Edward's presentation for Structural Genomics Consortium (SGC) meeting "Open Lab Notebooks: An Extreme Open Science Initiative" <a href="http://www.thesgc.org/open-lab-notebooks-2018">http://www.thesgc.org/open-lab-notebooks-2018</a> January 19, 2018 in Ottawa, Ontario, Canada.  Meeting supported by Canadian Institutes of Health Research (CIHR) and the Wellcome Trust.  Twitter hashtag was #SGCOpenNotebooks</p

Huntingtin construct design for BioID 2018/04/02

<p>Huntingtin structure-function open lab notebook project. Huntingtin construct design for BioID 2018/04/02.</p

Differential static light scattering (DSLS) of full-length huntingtin samples of different polyQ lengths (Q19, Q23, Q42 and Q54) – 2018/05/09

<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment</strong> - Differential static light scattering (DSLS) of full-length huntingtin samples of different polyQ lengths (Q19, Q23, Q42 and Q54) – 2018/05/09. To investigate how increased polyQ length of the huntingtin protein affects its biophysical properties. </p> <p> </p

HMGB1 and iVHH4 interaction analyses with huntingtin by pull-down - 15th January 2018

<p>Huntingtin structure function open lab notebook project. </p> <p>Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</p> <p> </p

Full-length HTT purification from HEK293T suspension culture using baculovirus transduction protocol for over-expression 2018/04/02

<p>Structure-function open lab notebook project. Full-length HTT purification from HEK293T suspension culture using baculovirus transduction protocol for over-expression 2018/04/02.</p

Preliminary PRC2-HTT interaction study 2017/06/02

<p>Huntingtin structure-function open lab notebook project.</p

Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged expression and purification 2018/04/02

<p>Huntingtin structure-function open lab notebook project. Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged, attempted expression and purification 2018/04/02</p

PRC2-HTT interaction study 20170719

<p>Huntingtin structure-function open lab notebook project</p

Mapping of phosphorylation modifications of insect cell derived huntingtin (2017/11/06)

<p>Huntingtin structure function open lab notebook</p

Large-scale full-length HTT Q19 and Q42 purification from HEK293T – 2018/04/16

<p>Huntingtin structure-function open lab notebook project. Large-scale full-length HTT Q19 and Q42 purification from HEK293T – 2018/04/16.</p