(A) Acrosome reaction of sperm from the epididymis and vas deferens was induced by calcium ionophore A23187

Acrosome-intact and -reacted sperm were subjected to western blot anaylsis with the anti-Shsp1/Mm.87328 antibody. AI, acrosome-intact sperm; AR, acrosome-reacted sperm. (B) Sperm from the epididymis and vas deferens were treated with 1% Triton X-100 and urea with different concentrations (2, 3, 4 and 6 M). Soluble and insoluble fractions after centrifugation of the treated sperm were subjected to western blot anaylsis with the anti-Sfap1/Mm.386907 and anti-Sfap2/Mm.157049 antibodies. Sup, supernatant after centrifugation; Pt, pellet after centrifugation.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p

Cells at different developmental stages were isolated, boiled in 3% SDS with 5% β-mercaptoethanol, subjected to SDS-PAGE and blotted with the specific antibodies

TC, testicular (spermatogenic) cells; TS, testicular sperm; S, mature sperm from the epididymis and vas deferens.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p

Testes from mutant mice (A) and a Sertoli cell line, TM4 (B), were subjected to protein extraction and western blot analysis using the specific antibodies

Total proteins from the testes were extracted by boiling the minced tissues in a protein sample buffer containing 3% SDS and 5% β-mercaptoethanol. ADAM2, with spermatogenic cell-specific expression, was included as a control. WT, wild type; TC, testicular (spermatogenic) cells.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p

Genomic, transcript, and protein characteristics of the genes in silico

<p><b>Copyright information:</b></p><p>Taken from "Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library"</p><p>http://www.biomedcentral.com/1471-2164/8/256</p><p>BMC Genomics 2007;8():256-256.</p><p>Published online 28 Jul 2007</p><p>PMCID:PMC1955454.</p><p></p> Gene structure and exon organization were determined using genome database searches. In exon organization, the boxes represent exons. The bars indicate regions amplified in the PCR analysis and used as probes in the Northern blot analysis. Coding regions were determined by selecting the longest open reading frames deduced from cDNA sequences. The predicted coding regions are shaded. The position of the poly A signal is marked by filled arrowheads, and the presence of poly A is indicated by 'A'. Chromosomal location was determined by searching the mouse and human genome databases. The predicted amino acid sequences of genes were analyzed using various bioinformatics tools (see Experimental Procedure). For annotation of genes with ontology terms, amino acid sequences were submitted to and subsequently obtained from exclusive web servers (Goblet), which use a variety of different protein databases and provide gene ontology codes. Each gene ontology code falls into one of the larger categories of molecular function (M), cellular component (C), or biological process (B)