Micro-scale {UHI} risk assessment on the heat-health nexus within cities by looking at socio-economic factors and built environment characteristics: The Turin case study (Italy)

Today the most substantial threats facing cities relate to the impacts of climate change. Extreme temperature such as heat waves and the occurrence of Urban Heat Island (UHI) phenomena, present the main challenges for urban planning and design. Climate deterioration exacerbates the already existing weaknesses in social systems, which have been created by changes such as population increases and urban sprawl. Despite numerous attempts by researchers to assess the risks associated with the heat-health nexus in urban areas, no common metrics have yet been defined yet. The objective of this study, therefore, is to provide an empirical example of a flexible and replicable methodology to estimate the micro-scale UHI risks within an urban context which takes into account all the relevant elements regarding the heat-health nexus. For this purpose, the city of Turin has been used as a case study. The methodological approach adopted is based on risk assessment guidelines suggested and approved by the most recent scientific literature. The risk framework presented here used a quantitative estimate per each census tract within the city based on the interaction of three main factors: hazard, exposure, and vulnerability. Corresponding georeferenced maps for each indicator have been provided to increase the local knowledge on the spatial distribution of vulnerability drivers. The proposed methodology and the related findings represent an initial stage of the urban risk investigation within the case study. This will include participatory processes with local policymakers and health-stakeholders with a view to guiding the local planning agenda of climate change adaptation and resilience strategies in the City of Turin

NAVIGATOR: an Italian regional imaging biobank to promote precision medicine for oncologic patients.

NAVIGATOR is an Italian regional project boosting precision medicine in oncology with the aim of making it more predictive, preventive, and personalised by advancing translational research based on quantitative imaging and integrative omics analyses. The project's goal is to develop an open imaging biobank for the collection and preservation of a large amount of standardised imaging multimodal datasets, including computed tomography, magnetic resonance imaging, and positron emission tomography data, together with the corresponding patient-related and omics-related relevant information extracted from regional healthcare services using an adapted privacy-preserving model. The project is based on an open-source imaging biobank and an open-science oriented virtual research environment (VRE). Available integrative omics and multi-imaging data of three use cases (prostate cancer, rectal cancer, and gastric cancer) will be collected. All data confined in NAVIGATOR (i.e., standard and novel imaging biomarkers, non-imaging data, health agency data) will be used to create a digital patient model, to support the reliable prediction of the disease phenotype and risk stratification. The VRE that relies on a well-established infrastructure, called D4Science.org, will further provide a multiset infrastructure for processing the integrative omics data, extracting specific radiomic signatures, and for identification and testing of novel imaging biomarkers through big data analytics and artificial intelligence

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field