Additional file 1: Table S1. of Understanding Phlebotomus perniciosus abundance in south-east Spain: assessing the role of environmental and anthropic factors

Percentage of “sticky” traps with sandflies (positive traps) and sand fly abundance in positive traps in dog kennels and sheep flocks in Murcia Region, southeast Spain in 2015. Table S2. Percentage of CDC traps with P. perniciosus and abundance in positive traps according to climatic variables from meteorological stations. A study of sand fly abundance in dog kennels and sheep flocks in Murcia Region, southeast Spain, in 2015. Table S3. Percentage of CDC traps with P. perniciosus and abundance in positive traps according to land use and soil and ground types. A study of sand fly abundance in dog kennels and sheep flocks in Murcia Region, southeast Spain, in 2015. (DOC 209 kb

Estimates of the multilevel linear regression model of the relationship between log transformed SGH OD values (multiplied by 100) and sampling time (model a), and <i>Leishmania</i> status and sampling time (model b).

<p>SE = standard error</p><p>Estimates of the multilevel linear regression model of the relationship between log transformed SGH OD values (multiplied by 100) and sampling time (model a), and <i>Leishmania</i> status and sampling time (model b).</p

Dynamics of IgG antibody response against sand fly salivary proteins in dogs naturally exposed to <i>P</i>. <i>perniciosus</i> over two years in endemic foci.

<p>Canine sera were tested by ELISA for the antibodies recognizing SGH (open circle), rSP03B protein (open diamond) and combination of rSP03B+rSP01 proteins (open square). Data are presented as median values for each sampling month. Asterisk represents significant change in the median compared to previous sampling. ODx100 = optical density multiplied by 100.</p

Dynamics of IgG antibodies recognizing SGH (A) and rSP03B protein (B) in individual dogs from CanL endemic locality.

<p><i>Leishmania</i> infection status was assigned for 53 dogs according their infection status at the end of the study in March third year and for 3 dogs according their infection status in July second year (dog number 7, 8, 49). OD values were log transformed and multiplied by 100. OD = optical density, neg = negative, exp = exposed, sub = subpatent, act = active.</p

Estimates of the multilevel linear regression model of the relationship between log transformed rSP03B OD values (multiplied by 100) and sampling time (model a), and <i>Leishmania</i> status and sampling time (model b).

<p>SE = standard error</p><p>Estimates of the multilevel linear regression model of the relationship between log transformed rSP03B OD values (multiplied by 100) and sampling time (model a), and <i>Leishmania</i> status and sampling time (model b).</p

Dynamics of <i>L</i>. <i>infantum</i> infection statuses in dogs from a CanL endemic site in southern Italy.

<p>Dogs were screened for <i>L</i>. <i>infantum</i> infection on four occasions, 56 dogs in July first year, 56 dogs in March the second year, 56 dogs in July the second year and 53 dogs in March the third year.</p

Correlations between IgG antibodies recognizing SGH and recombinant salivary proteins in dogs naturally exposed to sand flies.

<p>Correlation between SGH and rSP03B protein (A), between SGH and combination of rSP03B+rSP01proteins (B) and between rSP03B protein and combination of rSP03B+rSP01proteins (C) was performed using Spearman rank correlation. OD values were multiplied by 100. r = correlation index, CI = confidence interval.</p

Median and range antibody OD values (multiplied by 100) for each used antigen according sampling month.

<p>n = number of dogs</p><p>* significant change in the median compared to previous sampling</p><p>Median and range antibody OD values (multiplied by 100) for each used antigen according sampling month.</p

Excel spreadsheet including, in separate sheets, all numerical values used for Figs 1, 2, 3, 4 and S1.

Excel spreadsheet including, in separate sheets, all numerical values used for Figs 1, 2, 3, 4 and S1.</p

Annual cumulative incidence per 100,000 population of autochthonous human cutaneous leishmaniasis in European countries between 2005 and 2020.

Data based on cases reported to the WHO Global Health Observatory Data Repository.</p