List of primers used in the amplification and sequencing of the 18 S rDNA gene.

<p>List of primers used in the amplification and sequencing of the 18 S rDNA gene.</p

Photomicrographs of fresh spores of the novel myxosporean species.

<p>(A) <i>Henneguya maculosus</i> n. sp; (B) <i>Myxobolus flavus</i> n. sp.; (C) <i>Myxobolus aureus</i> n. sp.; (D) <i>Myxobolus pantanalis</i> n. sp.; (E) <i>Myxobolus umidus</i> n. sp.; (F) <i>Myxobolus piraputangae</i> n. sp. Scale bars = 10 µm.</p

Mean spore dimensions in µm ± standard deviation for the novel <i>Henneguya</i> and <i>Myxobolus</i> species from South America including their respective hosts and sites of infection.

<p>TL = tail length (only for <i>Henneguya maculosus</i> n. sp.); PCL = polar capsule length; PCW = polar capsule width; NFC = number of polar filament coils; dash = no data.</p

Similarity matrix for the 18 S rDNA sequences from all available <i>Henneguya</i> and <i>Myxobolus</i> species from South America adjusted for missing data (gaps).

<p>The lower triangular matrix shows the actual differences, while the upper triangular matrix shows the differences in terms of percentage of nucleotides. PC = <i>Pseudoplatystoma corruscans</i>; PR = <i>Pseudoplatystoma reticulatum</i>; PW = Pantanal wetland; MG = Mogi Guaçu River.</p

Maximum likelihood phylogenetic tree based on partial 18 S rDNA sequences.

<p>The bootstrap support values at branching points are listed as maximum likelihood/maximum parsimony. GenBank accession numbers are given following the name of the species. PW = Pantanal wetland; MG = Mogi Guaçu River; PC = <i>Pseudoplatystoma corruscans</i>; PR = <i>Pseudoplatystoma reticulatum</i>.</p

Schematic illustrations of the novel myxosporean species.

<p>(A) <i>Henneguya maculosus</i> n. sp.; (B) <i>Myxobolus flavus</i> n. sp.; (C) <i>Myxobolus aureus</i> n. sp.; (D) <i>Myxobolus pantanalis</i> n. sp.; (E) <i>Myxobolus umidus</i> n. sp.; (D) <i>Myxobolus piraputangae</i> n. sp. Scale bars = 5 µm.</p