Description Of An Animal Model Of Acute Cardiac Failure: In Vivo Experiments In Sheep

The purposes of this work were to, a) describe an acute animal model of severe cardiac failure induced by high doses of halothane, b) ana- lyze the effects of these overdoses of halothane on ar- terial wall dynamics and c) characterize the cardi- ovascular effects of halothane through the autonomic nervous system. Measurements were performed in six sheep before and after halothane administration (4%). A significative decrease was observed in mean aortic flow (P<0.05) and diameter (P<0.01) in heart failure with respect to control state (from 2.64±0.95 L.min-1 and 17.32±1.86 mm to 1.69±0.58 L.min-1 and 15.33±1.71 mm; respectively). A significantly de- crease was observed in mean (P<0.005), systolic (P<0.01) and diastolic (P<0.005) aortic pressure in heart failure (from 85.90±19.49 mmHg, 93.52±18.07 mmHg and 78.86±20.12 mmHg to 49.12±21.77 mmHg, 55.54±20.71 mmHg and 43.48±21.21 mmHg; respectively). Heart rate in control group (127.73±11.20 bpm) was significantly (P<0.05) higher than that observed in heart failure (107.15±13.53 bpm).Fil: Cabrera Fischer, Edmundo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; ArgentinaFil: Camus, Juan Manuel. Universidad de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barmak, Walter. Universidad Favaloro; ArgentinaFil: Risk, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diaz, Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: De Forteza, E.. Universidad Favaloro; Argentin

Description Of An Animal Model Of Acute Cardiac Failure: In Vivo Experiments In Sheep

The purposes of this work were to, a) describe an acute animal model of severe cardiac failure induced by high doses of halothane, b) ana- lyze the effects of these overdoses of halothane on ar- terial wall dynamics and c) characterize the cardi- ovascular effects of halothane through the autonomic nervous system. Measurements were performed in six sheep before and after halothane administration (4%). A significative decrease was observed in mean aortic flow (P<0.05) and diameter (P<0.01) in heart failure with respect to control state (from 2.64±0.95 L.min-1 and 17.32±1.86 mm to 1.69±0.58 L.min-1 and 15.33±1.71 mm; respectively). A significantly de- crease was observed in mean (P<0.005), systolic (P<0.01) and diastolic (P<0.005) aortic pressure in heart failure (from 85.90±19.49 mmHg, 93.52±18.07 mmHg and 78.86±20.12 mmHg to 49.12±21.77 mmHg, 55.54±20.71 mmHg and 43.48±21.21 mmHg; respectively). Heart rate in control group (127.73±11.20 bpm) was significantly (P<0.05) higher than that observed in heart failure (107.15±13.53 bpm).Fil: Cabrera Fischer, Edmundo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; ArgentinaFil: Camus, Juan Manuel. Universidad de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barmak, Walter. Universidad Favaloro; ArgentinaFil: Risk, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diaz, Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: De Forteza, E.. Universidad Favaloro; Argentin

A New Approach to Validate the Use of Brachial Blood Pressure to Assess Non-Invasive Aortic Pressure in Human Beings

Introduction: It has been demonstrated that the noninvasive evaluation of aortic blood pressure has a prognostic value but limited by the inaccuracy linked to technical errors and a differences in the pressure wave analysis.Aims: The aim of this study was to compare two methods used to validate the non-invasively central blood pressure waveforms obtained with an oscillometic device, with those recorded by intra-arterial measurements at the aortic level.Methods: In this study were included 20 subjects, 10 males (68 ± 12-years-old, BMI: 27.4 ± 4.6 Kg/m2) and 10 females (77 ± 8-years-old, BMI: 28.5 ± 5.3 Kg/m2). The analysed cohort was composed of patients with diagnosis of coronary artery disease. Invasive and non-invasive dataused in this research were previously analysed using a widely reported methodology and published by our group.The invasive aortic pressure recording was synchronically acquired with an oscillometric brachial acquisition and, then a reconstruction of central pressure wave was performed.In this research a correlation analysis using the entire aortic pressure cycle was performed.Results: Coefficient values found of the whole population, using the entire aortic pressure cycle, were similar to those obtained using the mean value of the cBP cardiac cycle was analysed (0.88 versus 0.89; respectively). On the contrary, the slope of the regression line determined by invasiveversus non-invasive cBP loops (n = 20) using the entire cBP cycle exhibit a remarkable decrease with respect to that obtained using the mean aortic pressure cycle (0.98 versus 0.77).Conclusions: In a first step, applying an interpolation procedure by means of oversampling and digital low pass filter, we found a high correlation between invasive and noninvasive instantaneous aortic pressure waveforms in: Men, women and the whole population, In a second step, resultsin terms of correlation coefficient and the slope derived from the regression analysis of invasive and non-invasive using a new data analysis allow to confirm high correlation coefficients and a more realistic slope value of the invasive versus non-invasive pressure wave relationship.Fil: Pessana, Franco Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Sanchez, Ramiro. Fundación Favaloro; ArgentinaFil: Lev, Gustavo. Fundación Favaloro; ArgentinaFil: Mirada, Micaela. Fundación Favaloro; ArgentinaFil: Mendiz, Oscar. Fundación Favaloro; ArgentinaFil: Ramírez, Agustín José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Cabrera Fischer, Edmundo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentin

Evaluation of Hemodynamic Autonomic Control in an Animal Model of Acute Heart Failure Induced by High Dose of Halothane

Acute animal models of cardiac failure are necessary to study new therapeutic options and should be thoroughly characterized from the hemodynamic point of view, including the response of the autonomic nervous system. Thus, the aim of this work was to characterize the pathophysiological adaptation of the autonomic nervous system to acute cardiac failure induced by high doses of halothane (4%). In six sheep, electrocardiogram, aortic pressure and flow were obtained and calculation of systemic vascular resistances was done. Variability analyses in the time and frequency domains were also performed. In the time domain, after heart failure induction using halothane 4%, a significant decrease of both aortic blood flow variability (from 0. 13 ± 0. 05 to 0. 09 ± 0. 02 L min -1, p < 0. 05) and the broad band spectra (from 1. 80 ± 0. 66 to 1. 25 ± 0. 57 L 2 min -2, p < 0. 005) was observed. Both mean RR (472 ± 44 to 567 ± 68 ms, p < 0. 01), and low frequency band of RR intervals (from 6. 2 ± 0. 9 to 7. 7 ± 1. 5 ms 2, p < 0. 05), showed a significant increase, and no change in systemic vascular resistance (from 54. 9 ± 29. 5 to 50. 3 ± 38. 4 mmHg min L -1), all of them after heart failure induction. We conclude that in this model of heart failure the autonomic nervous system activity is still functioning, the combination of increased mean and RR low frequency band, with no change in systemic vascular resistance suggest an increase in the sympathetic control (due to maintained SVR), in an attempt to compensate the depression in the cardiac activity and hemodynamic alterations after severe myocardial depression induced by halothane.Fil: Camus, Juan M.. Universidad de Mendoza. Facultad de Ingeniería; ArgentinaFil: Ramírez, Agustín José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; ArgentinaFil: Risk, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Tecnológico de Buenos Aires; ArgentinaFil: Palacios, Pablo J.. Universidad Favaloro; ArgentinaFil: de Forteza, Eduardo. Universidad Favaloro; ArgentinaFil: Cabrera Fischer, Edmundo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; Argentin

Carotid Intima Media Thickness Reference Intervals for a Healthy Argentinean Population Aged 11-81 Years

Reference intervals (RIs) of carotid intima media thickness (CIMT) from large healthy population are still lacking in Latin America. The aim of this study was to determine CIMT RIs in a cohort of 1012 healthy subjects from Argentina. We evaluated if RIs for males and females and for left and right carotids were necessary. Second, mean and standard deviation (SD) age-related equations were obtained for left, right, and average (left + right)/2) CIMT using parametric regression methods based on fractional polynomials, in order to obtain age-specific percentiles curves. Age-specific percentile curves were obtained. Males showed higher A-CIMT ( mm versus  mm, ) in comparison with females. For males, the equations were as follows: A-CIMT mean = 0.42 + ; A-CIMT SD = 5.9 × 10−2 + . For females, they were as follows: A-CIMT mean = 0.40 + ; A-CIMT SD = 4.67 × 10−2 + . Our study provides the largest database concerning RIs of CIMT in healthy people in Argentina. Specific RIs and percentiles of CIMT for children, adolescents, and adults are now available according to age and gender, for right and left common carotid arteries.Fil: Diaz, Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Bia, Daniel. Universidad de la República; UruguayFil: Zócalo, Yanina. Universidad de la República; UruguayFil: Manterola, Hugo Luis. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Larrabide, Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lo Vercio, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas; ArgentinaFil: del Fresno, Mirta Mariana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabrera Fischer, Edmundo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentin

Comparative analysis of arterial stiffness non invasively evaluated in hemodialyzed patients

pacientes hemodializados se producen en vasos elásticos y musculares pero sólo la Velocidad de la Onda del Pulso (VOP) aórtica ha demostrado ser un índice de alto valor pronóstico. Lo cual deja de lado a las arterias musculares. Los objetivos del estudio fueron: a) medir la VOP aórtica y la carotido-radial de pacientes hemodializados, y b) repetir el análisis anterior en la misma cohorte 5 años después, comparando cuatro índices diferentes de rigidez arterial. Material y métodos: a 23 pacientes hemodializados se les evaluó la VOP aórtica (VOPcf), la carotido-radial (VOPcr) y se calculó: la VOP centro-periférica (VOPcp), la diferencia (ΔVOP), el desacople de VOP y su cambio porcentual (%VOP). Las evaluaciones se hicieron en 2007 (Tiempo 1) y en 2012 (Tiempo 2). Resultados: La VOPcp mostró un aumento significativo entre la evaluación realizada entre el Tiempo 1 y el 2 (de 1.1±0.3 a 1.4±0.4; p<0.01). En los mismos tiempos ΔVOP mostró que los valores se incrementaban en términos negativos (de -0.9±3.0 a -2.7±2.9; p<0.05). El desacople de la rigidez centro-periférica mostró un significativo aumento (valores negativos) entre el Tiempo 1 y 2 (de 0.0±0.1 a -0.1±0.1; p<0.02). El %VOP ANÁLISIS COMPARATIVO DE LA RIGIDEZ ARTERIAL EVALUADA EN FORMA NO INVASIVA EN PACIENTES HEMODIALIZADOS COMPARATIVE ANALYSIS OF ARTERIAL STIFFNESS NON INVASIVELY EVALUATED IN HEMODIALYZED PATIENTS Cinthia Galli (1,2), Rodolfo Valtuille (3), Maia Daniela Percunte (2), Nahuel Hernán Carrizo (2), Daniel Bia (4), Ricardo Armentano (1,2), Edmundo Cabrera Fischer (1,2) 1) Área de Investigación y Desarrollo Universidad de Favaloro (AIDUF), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires 2) Facultad Regional Buenos Aires, Universidad Tecnológica Nacional, Buenos Aires 3) Fresenius FME Burzaco, Buenos Aires 4) Departamento de Fisiología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay Nefrología, Diálisis y Trasplante 2016; 36 (1) Pág. 26-33 entre ambas mediciones (valores negativos) mostró un significativo aumento (de -4.8±22.0 a -21.5±24.2; p<0.05). Conclusiones: en la presente investigación los índices de rigidez obtenidos en pacientes hemodializados, incluyendo arterias tanto elásticas y musculares, mostraron diferencias estadísticamente significativas cuando se compararon dos mediciones separadas por cinco años. Sin embargo los niveles de significación no fueron similares.Objectives: Changes in arterial stiffness in hemodialysis patients occur both, in elastic and muscular vessels but only the aortic Pulse Wave Velocity (PWV) has demonstrated to be a high prognostic value index, however, muscular arteries are not involved in the aortic PWV measurement. The purpose of this research was: a) to evaluate the aortic and carotid-radial PWV of hemodialysis patients, b) to repeat these measurements in the same cohort after 5 years comparing four different arterial stiffness indexes. Methods: 23 hemodialyzed patients carotid-femoral PWV (PWVcf) and carotidradial (PWVcr) were evaluated and calculations were as follows: PWV ratio, PWV difference (/ PWV), PWV mismatch and PWV percentage change (%PWV). These evaluations were performed using data obtained in 2007 (Time 1) and 2012 (Time 2). Results: PWV ratio showed a significant increase between measurements performed in Time 1 and 2 (from 1.1±0.3 to 1.4±0.4; p≤0.01). Similar increases in negative terms were found when /PWV was calculated from -0.9±3.0 to -2.7±2.9; p≤0.05) Calculated values of PWV mismatch increased significantly (negative values) between Time 1 and 2 (from 0.0±0.1 to -0.1±0.1; p≤0.02) Percent changes of PWV between Time 1 and 2 (negative values) showed a significant increase (from -4.8±22.0 to -21.5±24.2; p≤0.05). Conclusions: Stiffness indexes, obtained in hemodialyzed patients including both elastic and muscular arteries used in this research showed statistically significant differences when two measures with 5 years interval were compared. However significance levels were not similar.Fil: Galli, Cintia Nora. Universidad Tecnológica Nacional. Facultad Regional Buenos Aires; Argentina. Universidad Favaloro. Área de Investigación y Desarrollo; ArgentinaFil: Valtuille, Rodolfo. Fresenius Medical Care Burzaco; ArgentinaFil: Percunte, Maia Daniela. Universidad Tecnológica Nacional. Facultad Regional Buenos Aires; ArgentinaFil: Carrizo, Nahuel Hernán. Universidad Tecnológica Nacional. Facultad Regional Buenos Aires; ArgentinaFil: Bia, Daniel. Universidad de la República; UruguayFil: Armentano, Ricardo Luis. Universidad Tecnológica Nacional. Facultad Regional Buenos Aires; Argentina. Universidad Favaloro. Área de Investigación y Desarrollo; ArgentinaFil: Cabrera Fischer, Edmundo Ignacio. Universidad Favaloro. Área de Investigación y Desarrollo; Argentina. Universidad Tecnológica Nacional. Facultad Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Early preconditioning against reperfusion arrhythmias in a conscious sheep model. Role of ATP-dependent potassium channels

Faltan evidencias claras de precondicionamiento temprano (PT) contra las arritmias de reperfusión en animales conscientes. Objetivo: Analizar la protección del PT contra las arritmias de reperfusión en un modelo ovino consciente sometida a isquemia reversible y la participación de los canales de potasio ATP dependientes (KATP) como efectores del mecanismo protector. Material y Métodos: Se utilizaron ovejas adultas instrumentadas con un sensor de presión intraventricular, cables de marcapaso en aurícula derecha para registrar el electrocardiogrma, un oclusor neumático en tercio distal de arteria descendente anterior y microcristales piezoeléctricos en la zona isquémica para medir espesor parietal. A la semana de la cirugía se realizó el PT consistente en 6 episodios de isquemia reperfusión 45 min antes de una isquemia de 12 min seguida de 30 min de reperfusión. El índice de severidad de arritmias (ISA) de Lambeth se comparó con la situación control sin PT. La participación de los KATP se analizó administrando glibenclamida, inhibidor inespecífico de los KATP, y con inhibidores específicos de los KATP sarcolemales (HMR1098) y mitocondriales (5-HD), antes de la isquemia prolongada. Resultados: El PT redujo la incidencia de arritmias (p<0.01). La glibenclamida y el HMR1098 abolieron completamente la protección del PT y aumentaron el ISA y con el 5HD el efecto inhibitorio fue menor. El comportamiento mecánico acompañó la respuesta electrocardiográfica. Conclusiones: La participación de los KATP sarcolemales como efectores de la protección del PT contra las arritmias sugiere que las drogas inhibitorias utilizadas clínicamente, como por ejemplo la glibenclamida, se deberían usar con prudenciaEarly preconditioning (EP) against reperfusion arrhythmias is not clear in conscious animals. Objective: To analyze the EP protection against reperfusion arrhythmias in a conscious sheep model undergoing reversible ischemia and the role of ATP-dependent potassium channels (KATP) as effectors of the protective mechanism. Material and Methods: Adult sheep, prepared with an intraventricular pressure transducer, pacemaker wires in the right atrium to record the electrocardiogram, a pneumatic occluder in the distal third of the anterior descending coronary artery and piezoelectric microcrystals in the ischemic zone to measure wall thickness were used. One week after surgery, EP consisting of 6 periods of ischemia-reperfusion was performed 45 min before a 12 min ischemia followed by 30 min reperfusion. Lambeth’s arrhythmia severity index (ASI) was compared with controls without EP. The role of KATP channels was analyzed with glibenclamide, nonselective inhibitor of KATP channels, HMR1098 and 5-HD, specific inhibitors of sarcolemmal and mitochondrial KATP channels, respectively, before the prolonged ischemia. Results: EP reduced arrhythmias incidence (p<0.01). Glibenclamide and HMR1098 totally abolished EP protection, increasing ASI, and 5-HD had a lower inhibitory effect. Mechanical behavior matched the electrocardiographic response. Conclusions: The prominent sarcolemmal KATP channel participation as effectors of EP protection against arrhythmias suggests that clinically-used drugs which might close them should be used cautiously.Fil: Lascano, Elena. Universidad Favaloro; ArgentinaFil: Cabrera Fischer, Edmundo Ignacio. Area de Invest.y Desarrollo Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Negroni, Jorge Antonio. Universidad Favaloro; Argentin

Reference Values of Pulse Wave Velocity in South America: A Call to Action

The measurement of pulse wave velocity (PWV) is a wellknown technique currently considered the gold standard of arterial stiffness quantification due to its simplicity, accuracy, reproducibility, and predictive valueHowever, the available contemporary PWV normal values come mostly from multicenter registries obtained in Asia, USA and Europe. In South America, the reference values used are those derived from a European population, published by the Arterial Stiffness Collaboration Group. Finally, it is important to emphasize the need for epidemiological studies in South America, to provide reliable reference values of arterial stiffness, which still remains a challenge for our region.Fil: Diaz, Alberto Alejandro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Ramírez, Agustín José. Universidad Favaloro. Área de Investigación y Desarrollo; Argentina. Fundación Favaloro; ArgentinaFil: Cabrera Fischer, Edmundo Ignacio. Universidad Favaloro. Área de Investigación y Desarrollo; Argentin

Arterial stiffness in haemodialyzed patients: Findings and controversies

Vascular research in end-stage renal diseases is an interesting field in which the characterization of arterial stiffness proved to be valuable to predict morbidity and mortality. Particularly, patients on renal replacement therapy have been reported to have significant increases in arterial stiffness and cardiovascular mortality. The clinical relevance of the measurement of arterial stiffness is linked to therapeutical and preventive interventions. The purpose of this work is to analyze the results of the scientific research in the field of arterial stiffness, in which hemodialyzed patients were involved, emphasizing on clinical and in-vitro research carried out by our group compared to contributions previously reported in the specialized literature. These investigations are necessary to improve diagnostic strategies and monitor the arterial response to therapeutical interventions in chronic kidney disease.Fil: Cabrera Fischer, Edmundo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Zócalo, Yanina. Universidad de la República; UruguayFil: Wray, Sandra. Universidad de la República; UruguayFil: Bia, Daniel. Universidad de la República; Urugua

Reference intervals and percentile curve for left ventricular outflow tract (LVOT), velocity time integral (VTI), and LVOT-VTI-derived hemodynamic parameters in healthy children and adolescents: Analysis of echocardiographic methods association and agreement

Background: Echocardiographic reference intervals (RIs) for left ventricular outflow tract (LVOT) and velocity time integral (VTI) are scarce in pediatrics. Aims: (a) to generate RIs and percentiles for LVOT, VTI, and hemodynamic variables in healthy children and adolescents from Argentina; (b) to analyze the equivalence between stroke volume (SV), cardiac output (CO), and cardiac index (CI) obtained from two-dimensional echocardiography (2D) and LVOT-VTI analysis with pulsed wave Doppler (PWD); and (c) to analyze the association between subjects’ characteristics and VTI and LVOT-VTI-derived parameters. Methods: Two-dimensional and PWD studies were done in 385 subjects (5–24 years). Mean and standard deviation age-related and body surface area (BSA)-related equations were obtained for VTI and LVOT-VTI-derived parameters (parametric regression methods based on fractional polynomials). BSA- and age-specific percentiles were determined. Results: Pulsed wave Doppler- and 2D-derived parameters were positively correlated. However, PWD values were always lower than those from 2D. Specific RIs for PWD and 2D data were necessary. Covariance analysis showed that sex-specific RIs were required for LVOT, but not for VTI, VTI-derived CO and CI. Age-related RIs were obtained for LVOT, LVOT-VTI, and VTI-derived CO and CI. BSA-related RIs for VTI-derived CO and CI were obtained. Conclusions: Stroke volume, CO, and CI data from 2D and PWD are not equivalent. An accurate analysis of LVOT-VTI-derived parameters requires considering age and BSA. In this study, age- and BSA-related RIs and percentiles for LVOT, VTI, and hemodynamic parameters in healthy children and adolescents were determined, discriminating data according to the methodological approach (2D or PWD).Fil: Díaz, Alejandro. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Zócalo, Yanina. Universidad de la República; UruguayFil: Cabrera Fischer, Edmundo Ignacio. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Bia, Daniel. Universidad de la República; Urugua