Difference plot showing the <i>BRCA1</i> recurrent or founder mutations relative to the wild type controls.

<p>The melting profile of a wild type (WT) control is chosen as a horizontal base line and the relative differences in the melting of other samples are plotted relative to this baseline. Each trace represents the amplicon from a different individual's DNA sample. Melt curves of the <i>BRCA1</i> founder mutations (green/red) were plotted against melt curve of the wild type (blue).</p

Spectrum of <i>BRCA</i> pathogenic mutations identified.

<p> <b><i>Abbreviation</i></b><i>: SS, Splice-site mutation; NS, Nonsense mutation; FS, Frame-shift mutation; IFD, In-frame deletion mutation. Recurrent mutations are highlighted in bold.</i></p

HRM PCR primer sequences for the <i>BRCA</i> recurrent or founder mutations.

*<p>M13F: TGTAAAACGACGGCCAGT.</p>#<p>M13R: CAGGAAACAGCTATGACC.</p

Characteristics of all probands with <i>BRCA1</i>/<i>2</i> recurrent/founder mutations, with other mutations and without mutation (N = 451).

<p> <i>Abbreviations: F, Female; M, Male; GD, Guangdong; N/A, Not available; BC, Breast cancer; OC, Ovarian cancer;</i></p

Difference plot showing the three <i>BRCA2</i> founder mutations relative to the wild type controls.

<p>The melting profile of a wild type (WT) control is chosen as a horizontal base line and the relative differences in the melting of other samples are plotted relative to this baseline. Each trace represents the amplicon from a different individual's DNA sample. Melt curves of the <i>BRCA2</i> founder mutations (green/red) were plotted against melt curve of the wild type (blue).</p