Comparative evaluation of gene delivery devices in primary cultures of rat hepatic stellate cells and rat myofibroblasts

BACKGROUND: The hepatic stellate cell is the primary cell type responsible for the excessive formation and deposition of connective tissue elements during the development of hepatic fibrosis in chronically injured liver. Culturing quiescent hepatic stellate cells on plastic causes spontaneous activation leading to a myofibroblastic phenotype similar to that seen in vivo. This provides a simple model system for studying activation and transdifferentiation of these cells. The introduction of exogenous DNA into these cells is discussed controversially mainly due to the lack of systematic analysis. Therefore, we examined comparatively five nonviral, lipid-mediated gene transfer methods and adenoviral based infection, as potential tools for efficient delivery of DNA to rat hepatic stellate cells and their transdifferentiated counterpart, i.e. myofibroblasts. Transfection conditions were determined using enhanced green fluorescent protein as a reporter expressed under the transcriptional control of the human cytomegalovirus immediate early gene 1 promoter/enhancer. RESULTS: With the use of chemically enhanced transfection methods, the highest relative efficiency was obtained with FuGENE™6 gene mediated DNA transfer. Quantitative evaluation of representative transfection experiments by flow cytometry revealed that approximately 6% of the rat hepatic stellate cells were transfected. None of the transfection methods tested was able to mediate gene delivery to rat myofibroblasts. To analyze if rat hepatic stellate cells and myofibroblasts are susceptible to adenoviral infection, we have inserted the transgenic expression cassette into a recombinant adenoviral type 5 genome as replacement for the E1 region. Viral particles of this replication-deficient Ad5-based reporter are able to infect 100% of rat hepatic stellate cells and myofibroblasts, respectively. CONCLUSIONS: Our results indicate that FuGENE™6-based methods may be optimized sufficiently to offer a feasible approach for gene transfer into rat hepatic stellate cells. The data further demonstrate that adenoviral mediated transfer is a promising approach for gene delivery to these hepatic cells

Проект реконструкции ОРУ 220 кВ подстанции 500 кВ Томская

В процессе работы проводились расчеты затрат на оборудование и монтаж, расчёт экономической эффективности капиталовложений. Расчет показателей надежности распределительных устройств. Полученными результатами являются числовые значения необходимости реконструкции. Область применения: результаты данной работы могут быть использованы как образец для дальнейших реконструкций.In the process of work carried out calculations of the cost of equipment and installation, the calculation of the economic efficiency of investment. Calculation of reliability of switchgear. The results obtained are the numerical values of the need for reconstruction. Scope: the results of this work can be used as a model for further reconstruction

Проблемы научно-технического прогресса в бурении скважин: сборник докладов Всероссийской научно-технической конференции с международным участием , посвященной 60-летию кафедры бурения скважин, Томск, 2014 г.

В материалах сборника представлены результаты исследований научных работников по важным вопросам бурения геологоразведочных, технических, нефтяных и газовых скважин, проведения горно-разведочных выработок: разрушение горных пород, упрочнение породоразрушающего инструмента, скважинная гидродобыча руды, подземное выщелачивание урана, новые подходы по изучению буровых растворов, цементирование обсадных колонн, поиск новых путей получения информации с забоя в процессе бурения; даны решения ряда актуальных вопросов при проведении горноразведочных выработок

Data on Lipocalin 2 and phosphatidylinositol 3-kinase signaling in a methionine- and choline-deficient model of non-alcoholic steatohepatitis

The data presented in this brief report support the research article “Altered mitochondrial and peroxisomal integrity in lipocalin-2-deficient mice with hepatic steatosis” [1, doi: 10.1016/j.bbadis.2017.04.006]. We tested whether the absence of Lipocalin-2 (LCN2) could dysregulate the phosphatidylinositol 3-kinase/protein kinase B (PI3K-PKB) pathway and hepatic homeostasis in Non-Alcoholic-Steatohepatitis (NASH). The article highlights the role of LCN2 in hepatic homeostasis. Keywords: Lipocalin 2, Fat, MCD, NASH, PIP3, Oxidative stress, Mitochondri

Разработка модели финансирования инновационного проекта

Актуальность обусловлена растущим интересом со стороны компаний к вопросам эффективного финансирования собственных проектов, так как продуманное инвестирование в НИОКР повышает конкурентоспособность организации. Разработка бизнес-плана по получению гранта от фонда.Объектом исследования является финансовое планирование инновационного проекта. Предмет исследования – управленческо-экономические отношения в процессе формирования модели финансирования инновационного проекта.The relevance is due to the growing interest on the part of companies in the issues of effective financing of their own projects, as well-thought-out investment in R&D increases the competitiveness of the organization. Development of a business plan for obtaining a grant from the foundation.The object of the study is the financial planning of an innovation project. The subject of the study is management and economic relations in the process of forming a model for financing an innovative project