Inter-day (n = 8) accuracy and precision for TH and TAM spiked lysed Hep G2 cell extracts in 24-well plates.

<p>Inter-day (n = 8) accuracy and precision for TH and TAM spiked lysed Hep G2 cell extracts in 24-well plates.</p

Intra-day (n = 8) accuracy and precision for TH, TAM and TAc in spiked lysed PCCL3 cell extracts in 6-well plates.

<p>Intra-day (n = 8) accuracy and precision for TH, TAM and TAc in spiked lysed PCCL3 cell extracts in 6-well plates.</p

Mass balance in cells/supernatants for THM after incubation of mono-iodinated THM with PCCL3 rat thyrocytes.

<p>Mass balance in cells/supernatants for THM after incubation of mono-iodinated THM with PCCL3 rat thyrocytes.</p

Inter-day (n = 3) accuracy and precision for TH and TAM spiked lysed PCCL3 cell extracts in 6 well plates.

<p>Inter-day (n = 3) accuracy and precision for TH and TAM spiked lysed PCCL3 cell extracts in 6 well plates.</p

Thyronamine and thyronine chromatograms from Hep G2 cell lysate extracts spiked at 50 nM.

<p>2 MRM Traces per analyte, overlayed. Upper trace: T₀ (4.12 min); 3-T₁ (4.54 min); 3-T₁‘ (4.80 min); 3,5-T₂ (4.60 min); 3,3‘-T₂ (5.16 min); 3‘,5‘-T₂ (5.35 min); T₃ (5.01 min); rT₃ (5.64 min); T₄ (5.36 min). Lower trace: T₀AM (4.65 min); T₁AM (5.30 min); 3,5-T₂AM (5.34 min); T₃AM (5.97 min); rT₃AM (7.10 min); T₄AM (inset, 6.56 min).</p

Molecular structures of TH, TAM and TAc.

<p>Molecular structures of TH, TAM and TAc.</p

Negative ion electrospray tandem mass spectrometry and retention time (R_t) parameters for thyroacetic acids and internal standards.

<p>Negative ion electrospray tandem mass spectrometry and retention time (R_t) parameters for thyroacetic acids and internal standards.</p

Application of the LC-MS/MS method to extracts of PCCL3 rat thyrocytes.

<p>THM in extracts of PCCL3 (A, B) cell and supernatants (C, D) after incubation with 500 nM 3-T₁AM (A, C) or 3-T₁ (B, D) in Krebs-Ringer buffer as measured by LC-MS/MS. Bars represent means of 3 independent experiments carried out on different days +/- SEM.</p

Inter-day (n = 3) process efficiency, matrix effect and recovery for TH and TAM spiked lysed PCCL3 cell extracts in 6 well plates.

<p>Inter-day (n = 3) process efficiency, matrix effect and recovery for TH and TAM spiked lysed PCCL3 cell extracts in 6 well plates.</p

Inter-day (n = 8) process efficiency, matrix effect and recovery for TH and TAM spiked lysed Hep G2 cell extracts in 24-well plates.

<p>Inter-day (n = 8) process efficiency, matrix effect and recovery for TH and TAM spiked lysed Hep G2 cell extracts in 24-well plates.</p