Energy Content of Seeds of Palmer’s Pigweed (Amaranthus palmeri) in the Diet of Scaled Quail (Callipepla squamata) in Southeastern New Mexico

Palmer’s pigweed (Amaranthus palmeri) is a common grassland plant that occurs across much of North America. It is often considered a weed but is an important source of food for many game birds. We analyzed the energy content of seeds of Palmer’s pigweed obtained from the crops of scaled quail (Callipepla squamata) collected from plains-mesa sand-scrub habitat in Eddy and Lea counties, New Mexico. Seeds were dried for 48 hours at 60°C to remove moisture and then analyzed for gross caloric value (i.e., energy content) in an oxygen bomb calorimeter. Energy content of seeds of Palmer’s pigweed from New Mexico averaged 16.6 J/kg (4.0 kcal/g), and was among the lowest values obtained when compared to those of many seeds previously reported from the diet of scaled quail and other granivorous birds

Direct versus indirect detection in mSUGRA with self-consistent halo models

We perform a detailed analysis of the detection prospects of neutralino dark matter in the mSUGRA framework. We focus on models with a thermal relic density, estimated with high accuracy using the DarkSUSY package, in the range favored by current precision cosmological measurements. Direct and indirect detection rates are computed implementing two models for the dark matter halo, tracing opposite regimes for the phase of baryon infall, with fully consistent density profiles and velocity distribution functions. This has allowed, for the first time, a fully consistent comparison between direct and indirect detection prospects. We discuss all relevant regimes in the mSUGRA parameter space, underlining relevant effects, and providing the basis for extending the discussion to alternative frameworks. In general, we find that direct detection and searches for antideuterons in the cosmic rays seems to be the most promising ways to search for neutralinos in these scenarios.Comment: 26 pages, 9 figure

High throughput nanopore sequencing of SARS-CoV-2 viral genomes from patient samples

In late 2019, a novel coronavirus began spreading in Wuhan, China, causing a potentially lethal respiratory viral infection. By early 2020, the novel coronavirus, called SARS-CoV-2, had spread globally, causing the COVID-19 pandemic. The infection and mutation rates of SARS-CoV-2 make it amenable to tracking introduction, spread and evolution by viral genome sequencing. Efforts to develop effective public health policies, therapeutics, or vaccines to treat or prevent COVID-19 are also expected to benefit from tracking mutations of the SARS-CoV-2 virus. Here we describe a set of comprehensive working protocols, from viral RNA extraction to analysis using established visualization tools, for high throughput sequencing of SARS-CoV-2 viral genomes using a MinION instrument. This set of protocols should serve as a reliable "how-to" reference for generating quality SARS-CoV-2 genome sequences with ARTIC primer sets and long-read nanopore sequencing technology. In addition, many of the preparation, quality control, and analysis steps will be generally applicable to other sequencing platforms