Pancreatic Ductal Adenocarcinoma Associated with Autoimmune Pancreatitis

Autoimmune pancreatitis (AIP), in contrast to other benign chronic pancreatic diseases, can be cured with immunosuppressant drugs, thus the differentiation of AIP from pancreatic cancer is of particular interest in clinical practice. There is the possibility that some patients with AIP may develop pancreatic cancer, and this possibility contributes to increasing our difficulties in differentiating AIP from pancreatic cancer. We herein report the case of a 70-year-old man in whom pancreatic adenocarcinoma and AIP were detected simultaneously. We must carefully monitor AIP patients for the simultaneous presence of pancreatic cancer, even when a diagnosis of AIP is confirmed

Viability of cloned bovine embryos after one or two cycles of nuclear transfer and in vitro culture

We described an exclusively in vitro procedure for cloning and recloning bovine embryos. Embryos obtained by IVM/IVF/IVC developed to the morula stage were used as blastomere donors in cunjunction with IVM recipient oocytes. Reconstructed embryos were developed in vitro in co-culture using bovine oviductal epithelial cells. The resulting morulae were used as donors for recloning under the same experimental conditions. No significant difference was observed between cloning and recloning in terms of development (rates of blastocysts: 12.9 versus 14.9%), in the number of nuclei per blastocyst (63.8 versus 49.1), or in pregnancy rates (35.7 versus 33.3%). The high variability observed between replicates and the correlation between results in first and second cycle nuclear transfer may suggest an inherant potential of individual donor embryos to support development by clonin

Nuclear transplantation using bovine primordial germ cells from male fetuses.

The developmental potential of nuclei of bovine gonial cells was investigated by nuclear transfer. Gonial cells were collected from male fetuses at about 175 days post coitum (p.c.). They were fused with enucleated oocytes; reconstituted embryos were cultured in vitro for 7 days. Embryos reaching the compacted morula or blastocyst stage were either fixed for cell counting or transferred into recipients. Out of 115 oocyte-gonia fusions, 101 (87.8%) gave rise to cleaved embryos at Day 3 and 26 (22.6%) had reached the 8-cell stage. At Day 7, 1 (1%) developed to the morula stage and 5 (4%) reached the blastocyst stage. Three blastocysts were fixed and showed normal cell numbers (135; 90; 76 cells). Three blastocysts and one morula were transferred in four recipients; two recipients were pregnant at Day 21 but only one was positive at Day 35 p.c.; this last one aborted around Day 40 p.c. No conceptus was collected. These results indicate that gonial cell nuclei can be partially reprogrammed; they are able to develop into blastocysts and to initiate gestation. However, more experiments will be necessary to prove the nuclear totipotency of bovine gonial cells

Vitrification of day 6 in vitro produced bovine embryos as donors for cloning by nuclear transfer.

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Fractionation and partial characterization of proteins extracted from the bovine fallopian tube: preparation of tools for further purifications

Described in the present paper is a combined biochemical and immunological approach to study oviductal proteins in the bovine. Antisera were raised against semi-purified proteins extracted from bovine tubal mucosal tissue and were characterized. These antisera are available to monitor puritications of specific oviductal proteins in the future. Oviducts from 170 cyclic cows were collected at a slaughterhouse, and high amounts of mucosal proteins were extracted. The proteins were fractionated after precipitation with ammonium sulfate, anti-bovine serum albumin (bSA) and anti-bovine immunoglobulins bIg) afiinity chromatography and ion exchange chromatography. Each of the 12 fractions obtained after ion exchange chromatography was used to immunize a rabbit. Conditioned media were recovered from bovine oviduct cell monolayers cultured without serum to cot&m the oviductal origin of the extracted proteins. After Western blot analysis, 15 proteins were detected in the bovine oviductal extracts, and their molecular weights and isoelectric points were determined by 2 dimensional electrophoresis. Among these 15 proteins, 11 were also detected in conditioned media of bovine oviductal cells. These results demonstrate an oviductal origin of the 11 detected proteins and strongly suggest their secretion by the oviductal cells

Medetomidine Ketamine Anesthesia in Cats

The medetomidine/ketamine combination was evaluated as an anaesthetic in cats undergoing ovariectomy. It was compared with acepromazine/ketamine, xylazine/ketamine and zolazepam/tiletamine combinations. 60 animals, divided into 6 groups of 10 animals were involved. Medetomidine highly potentiated the anaesthetic effects of ketamine and balanced the two main disadvantages of this drug: weak muscle relaxation and poor analgesia in deep organs. 80 micrograms medetomidine/kg--5 mg ketamine/kg provided a duration of anaesthesia longer than 1 mg acepromazine/kg--10 mg ketamine/kg, and not significantly different from those induced by 1 mg xylazine/kg--10 mg ketamine/kg or 7.5 mg zolazepam/kg--7.5 mg tiletamine/kg. Furthermore, it produced a better muscle relaxation than acepromazine/ketamine and zolazepam/tiletamine combinations. The analgesia in deep organs observed after medetomidine/ketamine administration appeared to be more substantial than that observed after acepromazine/ketamine or xylazine/ketamine combinations. The stimulating effect on heart rate of ketamine compensated the bradycardiac effect of medetomidine. Nevertheless, the administration of 80 micrograms medetomidine/kg--5 mg ketamine/kg resulted as with xylazine/ketamine, in decreasing heart rate whereas acepromazine/ketamine and zolazepam/tiletamine combinations did not exhibit bradycardiac effect. It was concluded that the medetomidine/ketamine combination provided a suitable anaesthesia for cats characterized by rapid induction, good muscle relaxation, good analgesia and bradycardia

Apnoea or hypoventilation during various experimental conditions of extracorporeal oxygenation

The role of neural and humoral factors in the control of spontaneous ventilation under extracorporeal circulation was studied in 18 anaesthetized dogs. During high flow veno-arterial bypass (90±10 ml/min/kg) a prolonged apnoea or marked hypoventilation was produced whenever the carotid PO2 was above 75±10 mmHg. Hypercapnia did not exert any significant influence on ventilation. In a medium flow (60±18 ml/min/kg), hypoventilation appeared when the carotid PO2 and PCO2 were in the normal range. When the carotid PO2 was below or when the carotid PCO2 was above normal range, the animals hyperventilated. Thus under all circumstances, hypoxaemia appeared to maintain its determinant role in the control of respiration. However hypercapnia did not play a significant role during high flow veno-arterial bypass. The bilateral sections of cranial nerves IX, X and XI did not modify the phenomena described above, suggesting that the known peripheral baroreceptors and chemoreceptors are not responsible for changes in ventilatory patterns under extracorporeal circulation.SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe