4 research outputs found
The importance of markers HLA6 and CD68 in placenta tissues of recurrent pregnancy loss
Introduction: Recurrent pregnancy loss of unknown etiology is correlated with immunological factors during pregnancy. Changes in leukocyte subpopulations and HLA (Human Leukocyte Antigen) expression take place in pregnant uterus on both decidua basalis and decidua parietalis in order to carry the semiallogenic embryo. These changes affect the pregnancy course.Objective: Our research is focused to enlighten the immunological changes that take place in the uterus of women with recurrent abortions of unknown etiology during first trimester of pregnancy.Materials and methods: The miscarriage group was obtained from 25 women who miscarried between the ages of 35 to 42 years and controls consisted of 25 healthy women between the ages of 27 to 39 years, who had electively terminated their pregnancies during first trimester of pregnancy. The abortion was processed and specimens taken were studied, using immunohistochemical methods. Specimens were taken from decidua basalis and decidua parietalis. Monoclonal antibodies were used against HLAG (Human Leukocyte Antigen G) and CD68 (Cluster of Differentiation 68). The results were statistically analysed with Mann-Whitney test.Results: HLA-G expression in decidua basalis from miscarriage group was found decreased.CD68 + cell expression was found increased in both deciduas from the miscarriage group.Conclusion: The immunological profile of women with recurrent miscarriage is quite different comparing with controls. A possible role of CD68+cells in RPL was observed .Changes in HLA-G expression was observed
Expression of peroxisome proliferator activation receptors (PPARs) and TNFα in placenta tissues in unexplained recurrent pregnancy loss: An immunohistochemical study
Introduction. PPAR expression in placenta
tissues regulates proinflammatory cytokine production
and preserves the quiescence of the uterus during
pregnancy. PPAR-γ regulates inflammatory response
during gestation while PPAR-δ and TNFα play a central
role at implantation, decidualization and placentation.
However, their expression levels affect normal
pregnancy and may cause gestational complications and
miscarriage. The aim of this report is to investigate the
relationship of these molecules with unexplained
recurrent miscarriage.
Materials-methods. The miscarriage group was
obtained from 12 women, between the ages of 35 to 42
years, who miscarried during the 1st trimester of
gestation and controls consisted of 12 healthy women,
between the ages of 27 to 39 years, who had electively
terminated their pregnancies, during the 1st trimester of
gestation. The abortion material was processed and
specimens taken were studied using immunohistochemical methods. Specimens were taken from decidua
basalis and decidua parietalis. Monoclonal antibodies
were used against PPAR-γ (Peroxisome Proliferator
Activation Receptor γ), PPAR-δ and TNFα (Tumor
Necrosis Factor alpha). The results were statistically
analyzed with Mann-Whitney test.
Results. Our research identified PPAR-γ expression
in decidua basalis and decidua parietalis from control
group and decidua basalis from miscarriage group.
PPAR-δ expression was also identified in both deciduas
from both groups. Statistically, no significant change in
PPAR-γ and PPAR-δ expression was observed between
recurrent miscarriage group and controls. On the
contrary, a statistically significant upregulation of TNFα
was identified in both deciduas between miscarriage
group and controls (p<0.05).
Conclusions. Our evidence did not support a
possible role of PPARs expression in recurrent
pregnancy loss. However, a potential involvement of
TNFα in the syndrome was reported. Further research
should be performed due to insufficient bibliographic
data
Low expression of Progesterone Receptor A in intermediate trophoblast of miscarriages
Summary. Objective: To examine the potential
differences in the expression of Progesterone Receptor A
and Estrogen Receptor A in intermediate trophoblastic
cells at the implantation site in elective abortions and
miscarriages by immunohistochemistry.
Study Design: Twenty two (22) samples of
miscarriages and eighteen (18) samples of elective
abortions were obtained during gestational weeks 6 to
12. Monoclonal antibodies against Cytokeratin 7 and
prolactin were used to help discriminate between
trophoblastic and decidual cells at the feto-maternal
interface on formalin-fixed paraffin-embedded sections.
Samples were then stained with ERA and PRA
antibodies. Nuclear expression was considered positive.
Staining intensity was measured according to a 4 grade
scale. Statistical analysis of the results was performed
using the Mann-Whitney test and the Wilcoxon signed
rank test.
Results: PRA expression in intermediate
trophoblastic cells was significantly higher in elective
abortions (control group) compared to miscarriages.
ERA expression was uniformly negative in both groups.
Conclusion: PRA expression is significantly lower
in intermediate trophoblastic cells of miscarriages
compared to elective abortion pregnancies. Although
this could be solely a result of a secondary event, it is
still an important finding in the effort to unravel the
complex molecular pathobiology of spontaneous
abortions
Immunohistochemical study of immunological markers: HLAG, CD16, CD25, CD56 and CD68 in placenta tissues in recurrent pregnancy loss
Introduction: Recurrent pregnancy loss
(RPL) of unknown etiology is correlated with
immunological alterations during pregnancy. Normally,
changes in leukocyte subpopulations and HLA
expression take place in pregnant uterus in order to
tolerate the semi-allogenic embryo. Objective: Our
research tries to enlighten the immunological changes
that take place in the uterus of women with recurrent
abortions of unknown etiology during first trimester of
pregnancy. Materials and methods: The miscarriage
group was obtained from 25 women who miscarried
between the ages of 35 to 42 years and controls
consisted of 25 healthy women between the ages of 27 to
39 years, who had electively terminated their
pregnancies during the first trimester of pregnancy. The
abortion was processed and specimens taken were
studied using immunohistochemical methods.
Specimens were taken from decidua basalis and decidua
parietalis. Monoclonal antibodies were used against
HLAG (Human Leukocyte Antigen G), CD68( Cluster of
Differentiation 68), CD56, CD16 and CD25. The results
were statistically analysed with Mann-Whitney test.
Results: HLA-G expression in decidua basalis from
miscarriage group was found to be decreased. CD25+
cell expression was found to be invariable in deciduas
from both groups. CD16+ cell and CD68 + cell
expression was found to be increased in deciduas from
the miscarriage group. CD56+ cell expression was found
to be increased in decidua parietalis from miscarriage
group. Conclusion : Several differences in the
immunological profile of deciduas from RPL group were
observed. Changes in feto-protective HLA-G expression
and a possible implication of macrophages and NK cells
were found