Screening de l’activité bionématicide de différents extraits de Thymus zygis

Extraction and chromatographic analysis of thyme extracts have been made. The GC-MS analysis allowed the identification of several molecules and their proportions contained in the different extracts of T. zygis. The bionématicide activity of these extracts was evaluated against root-knot nematodes, Meloidogyne spp. The aqueous, methanol, ethanol extracts and the essential oil were tested with different concentrations in vivo and in vitro on second stage J2. The direct treatment in vitro with the four extracts showed a nematicide effect on J2 juveniles ranging from 33% to 69.7% after 72 hours of incubation with a maximum mortality rate for the essential oil. For in vivo test, the same extracts were applied to the roots of infested tomato. The aqueous extract, methanol extract and essential oil gave the best nematicide effect (gall index 0 and the number of J2/100g soil: 11, 11.5 and 14 respectively).L’extraction ainsi que l’analyse chromatographique des extraits du thym ont été réalisées. L’analyse CPG-MS a permis l’identification de plusieurs molécules ainsi que leurs proportions contenues dans les différents extraits du T. zygis. L'activité bionématicide de ces extraits de Thymus zygis contre les nématodes à galles Meloidogyne spp. a été évaluée. Les extraits aqueux, méthanolique, éthanolique et l’huile essentielle ont été testés avec différentes concentrations in vivo et in vitro sur les juvéniles J2. Le traitement direct in vitro avec les quatre extraits a montré un pouvoir nématicide contre les juvéniles J2 variant de 33% à 69,7% après 72 heures d’incubation avec un taux de mortalité maximal pour l’huile essentiel. Pour le test in vivo les mêmes extraits ont été appliqués aux racines de la tomate infestée. L’extrait aqueux, l’extrait méthanolique et l’huile essentielle ont donné le meilleur effet nématicide (indice de galle : 0 et le nombre de J2/100g de sol : 11 ; 11,5 et 14 respectivement)

Acinos alpinus and Ziziphora hispanica: Phenolic Profile, Antioxidant and Antibacterial Properties of Hydromethanolic Extracts from Aerial Parts

Acinos alpinus and Ziziphora hispanica belonging to the Lamiaceae family are well-known for their biological properties. The phenolic composition was characterized using HPLC coupled to photodiode array and electrospray ionization mass spectrometry. The antioxidant capacity was assessed by 2,2′-diphenyl-1-picrylhydrazyl radical and ferric reducing antioxidant power assays. The antibacterial activity was evaluated by disc-diffusion and broth micro-dilution methods. A total of 53 phenolic compounds were identified in both extracts, with the predominance of caffeoylquinic acid isomers with a content of 2.09 mg/g in A. alpinus extract, while coumarylquinic acid isomers with a content of 17.33 mg/g was determined in Z. hispanica extract. The Acinos alpinus extract displayed the highest radical scavenging activity (IC50 DPPH = 3.226±0.035 mg/mL) and reducing power (EC50 FRAP = 3.792±0.001 mg/mL), followed by Z. hispanica extract with IC50 DPPH value of 7.265±0.209 mg/mL, and EC50 FRAP value of 24.689± 0.106 mg/mL. Furthermore, both A. alpinus and Z. hispanica extracts exhibited a bactericidal effect against six pathogenic bacteria with a MIC value from 4.16 to 33.33 mg/mL. On the basis of the results achieved, the aerial parts of Moroccan Acinos alpinus and Ziziphora hispanica might be used as natural agents in the food and pharmaceutical field