Additional file 1: of Murine breast cancer feed arteries are thin-walled with reduced α1A-adrenoceptor expression and attenuated sympathetic vasocontraction

Figure S1. Vasocontraction is attenuated in breast cancer feed arteries compared to matched control arteries under acidic conditions. Concentration-dependent contractions in response to norepinephrine (A), endothelin-1 (B), and elevated [K+]o (C) at pHo 6.8 (n = 8). For norepinephrine and K+, contractions elicited in the same arteries at pHo 7.4 are shown for comparison. Because of slow relaxation of arteries after washout of endothelin-1, vasocontraction to endothelin-1 was only tested at one level of pHo for each artery. Experiments with elevated [K+]o were performed in presence of 1 μmol/L phentolamine in order to avoid effects of norepinephrine released from sympathetic nerve endings in response to depolarization. Curves are results of least-squares fits to sigmoidal functions and compared using extra-sum-of-squares F tests. **P < 0.01, ***P < 0.001 vs. control, both at pHo 6.8. (PDF 244 kb

Additional file 1 of Carbonic anhydrases reduce the acidity of the tumor microenvironment, promote immune infiltration, decelerate tumor growth, and improve survival in ErbB2/HER2-enriched breast cancer

Additional file 1. Supplementary tables and figures. Table S1 summarizes the clinical and pathological patient characteristics. Table S2 and S3 provide sequence information for primers and probes used for quantitative RT-PCR analyses. Table S4 and S5 give detailed information on the statistics analyses in Figs. 1 and 9. Figure S1 shows patterns of gene expression for carbonic anhydrases across breast cancer molecular subtypes. Figure S2 and S3 provide survival curves for Luminal A (Fig. S2) and Luminal B (Fig. S3) breast cancer patients stratified for carbonic anhydrase expression