A novel technique to characterize conformational state of the proteins: p53 analysis

4noAs the technology is advancing, biotechnologist and pharmacologist seems more interested and focused towards the development of innovative sensing solution/technology capable of evaluating proteins without any limitations of time and cost which were encountered/offered by conventional/traditional methods such as ELISA used for protein quantification. To allow continuous monitoring and attain protein sample information in a non-invasive way, spectrophotometry might be considered as an alternate method which analyzes different conformational states of proteins by closely observing the variation in optical properties of the sample. The work presented studies p53 protein conformational dynamics and their involvement in various pathophysiological and neurodegenerative disease/disorders using the spectrophotometer-based method. By utilizing the technique of spectrophotometry, investigations were carried out on three samples containing varied molecular state of p53 (Wild p53, Denatured p53, and Oxidized p53), to detect the difference in light absorption. Overall, this proposes the possibility of a simple, non-invasive and optical based method capable of detecting and identifying different structural states of p53 while overcoming the complexities offered by the conventional procedures.nonenoneAbdullah, Saad*; Serpelloni, Mauro; Abate, Giulia; Uberti, DanielaAbdullah, Saad; Serpelloni, Mauro; Abate, Giulia; Uberti, Daniel

Gold nanoparticles-reduced graphene oxide based electrochemical immunosensor for the cardiac biomarker myoglobin

A composite consisting of gold nanoparticles and reduced graphene oxide (AuNPs@rGO) was electrochemically prepared in-situ on a screen printed electrode (SPE) which then was used as an immunosensor for the cardiac biomarker myoglobin. The nanocomposite was characterized by transmission electron microscopy (TEM, scanning electron microscopy (SEM), atomic force microscopy, FTIR and electrochemical impedance spectroscopy. For FTIR, TEM and SEM, the deposition was done on indium tin oxide coated glass plates. The immunosensor was obtained by immobilization of in-house generated antibody against cardiac myoglobin on the electrode surface. The immunosensing response was monitored using differential pulse voltammetry, which showed a reduction peak at ~ −0.5 V (vs. Ag/AgCl). The reduction peak arises from the reduction of iron moiety present in the heme group of myoglobin. The immunosensor exhibited dynamic linearity range from 1 ng.mL−1 to 1400 ng.mL−1 with the detection limit of ~0.67 ng.mL−1 for cardiac myoglobin. The obtained result was almost eight times better (in terms of detection limit) than that obtained with ELISA tests (with detection limit of ~4 ng.mL−1) using the same antibodies. The immunosensor was applied to analyze spiked serum samples also

Acetylcholinesterase (AChE) Biosensors for Determination of Carbamate Pesticides

Applications of nanotechnology are the remarkable sizes dependent on physiochemical properties of nanomaterials that have led to the developed protocols for synthesizing nanomaterials over a range of size, shapes and chemical compositions. Nanomaterials are normally powders composed of nanoparticles which exhibit properties that are different from powders. Nanotechnology is the engineering of functional systems at the molecular scale with their wide applications in energy sector, including -but not limited to- energy resources, energy conversion, energy storage, and energy usage; drug delivery systems including- safety concerns, perspective, challenges, target therapeutics for cancer, neurodegenerative diseases and other human diseases, nanomaterials based tissue engineering; and food sectors including to- food safety and quality, opportunities, challenges, nanomaterials based enhancing food packing, and determination of foodborne pathogens, agro and marine food, analysis of market, regulations and future prospects