Effects of waste engine oil spillage on soil physico-chemical and microbiological properties

Changes in physic-chemical and microbial properties of soils contaminated with waste motor oil were monitored over a 24 week period. Oil application to soils resulted in a decrease in moisture content but brought about increase in organic matter, total nitrogen and available phosphorus contents. There was an initial decrease in microbial counts followed by a subsequent increase in population levels after four weeks. Microbial species diversity was however reduced in oil-contaminated sites relative to the control sites. Hydrocarbon-utilizing bacteria isolated from the experimental sites were identified as Pseudomonas, Acinetobacter, Alcaligenes, Flavobacterium and Corynebacterium. The organisms grew on long-chain n-alkanes, crude oil and fresh engine oil while a few species grew on aromatic hydrocarbons. Laboratory biodegradation studies of fresh engine oil using strains of Pseudomonas, Acinetobacter, and Corynebacterium showed a progressive decrease in oil concentration and pH of the medium due to the production of acidic metabolites

Occurrence and Distribution of Sulphate-Reducing Bacteria in a Polluted Lagoon

The presence and distribution of sulphate-reducing bacteria (SRB) in the sediment and water from four strategic points on the Lagos Lagoon were assessed using the most probable number technique. All the samples were positive for the dissimilatory sulphate reducers. The relative occurrence varied markedly with the site and pollution status. The least polluted end of the lagoon recorded the lowest number of SRB in both sediment (4.23x102 SRB/ml) and water (28 SRB/ml) while the most polluted site receiving sewage and domestic wastes harboured numbers as high as 5.71x103 and 7.6x104 SRB/ml for water and sediment sasmples, respectively. The occurrence of sulphate-reducing bacteria in such numbers indicated that this environment favoured biocorrosion of buried and immersed metals. The active involvement of these organisms in the syngenesis of sulphur, metallic sulphides and the general biodegradation of pollutants in this ecosystem is discussed