Male Microchimerism at High Levels in Peripheral Blood Mononuclear Cells from Women with End Stage Renal Disease before Kidney Transplantation

Patients with end stage renal diseases (ESRD) are generally tested for donor chimerism after kidney transplantation for tolerance mechanism purposes. But, to our knowledge, no data are available on natural and/or iatrogenic microchimerism (Mc), deriving from pregnancy and/or blood transfusion, acquired prior to transplantation. In this context, we tested the prevalence of male Mc using a real time PCR assay for DYS14, a Y-chromosome specific sequence, in peripheral blood mononuclear cells (PBMC) from 55 women with ESRD, prior to their first kidney transplantation, and compared them with results from 82 healthy women. Male Mc was also quantified in 5 native kidney biopsies obtained two to four years prior to blood testing and in PBMC from 8 women collected after female kidney transplantation, several years after the initial blood testing. Women with ESRD showed statistically higher frequencies (62%) and quantities (98 genome equivalent cells per million of host cells, gEq/M) of male Mc in their PBMC than healthy women (16% and 0.3 gEq/M, p<0.00001 and p = 0.0005 respectively). Male Mc was increased in women with ESRD whether they had or not a history of male pregnancy and/or of blood transfusion. Three out of five renal biopsies obtained a few years prior to the blood test also contained Mc, but no correlation could be established between earlier Mc in a kidney and later presence in PBMC. Finally, several years after female kidney transplantation, male Mc was totally cleared from PBMC in all women tested but one. This intriguing and striking initial result of natural and iatrogenic male Mc persistence in peripheral blood from women with ESRD raises several hypotheses for the possible role of these cells in renal diseases. Further studies are needed to elucidate mechanisms of recruitment and persistence of Mc in women with ESRD

Overview of recent alcator C-Mod results

Research on the Alcator C-Mod tokamak is focused on exploiting compact high density plasmas to understand core transport and heating, the physics of the H mode transport barrier, and the dynamics of the scrape-off layer and divertor. Rapid toroidal acceleration of the plasma core is observed during ohmic heated H modes and indicates a momentum pinch or similar transport mechanism. Core thermal transport observations support a critical gradient interpretation, but with gradients that disagree with present theoretical values. High resolution measurements of the H mode barrier have been obtained, including impurity and neutral densities, and the instability apparently responsible for the favourable 'enhanced D alpha' regime has been identified. Divertor bypass dynamic control experiments have directly addressed the important questions surrounding main chamber recycling and the effect of divertor closure on impurities and confinement. Future plans include quasi-steady-state advanced tokamak plasmas using lower hybrid current drive