30 research outputs found
miRNA-Expression in Tonsillenkarzinomen im Kontext von HPV-Infektion und Expression der Antileukoproteinase SLPI
Der Einfluss des Rauchverhaltens auf den HPV-Status und SLPI (secretory leukocyte protease inhibitor) Expression in Kopf-Hals-Karzinomen
Regionale HPV-Prävalenz-Heterogenität bei Kopf-Hals-Karzinomen (HNSCC) in Abhängigkeit von der Primärtumorlokalisation
miRNA-Expression in Tonsillenkarzinomen im Kontext von HPV-Infektion und Expression der Antileukoproteinase SLPI
Die Rolle der Antileukoproteinase SLPI bei der Infektion mit HPV bei Kopf-Hals-Karzinomen
HPV-Infektionen in Kopf-Hals-Karzinomen: Prävalenzunterschiede in Abhängigkeit von Anatomie und Geographie
Einfluss von HPV auf das Überleben von Patienten mit Tonsillenkarzinomen nach primärer mikroendoskopisch-chirurgischer Therapie mit dem CO2-Laser (MCCL)
SLPI- und Annexin A2-Expression in neoplasiefreiem Tonsillengewebe in Korrelation zum Rauchverhalten der Patienten
Immunolocalization of Na/SO4-cotransport (NaSi-1) in rat kidney.
The proximal tubule is the major site for renal reabsorption of sulphate. A sodium-dependent transport system for sulphate (NaSi-1) has recently been identified from a rat kidney cortex cDNA library. Recent work demonstrated that NaSi-1 mRNA is expressed predominantly in proximal tubules. In the present work expression along the nephron of the Na/SO4-cotransporter NaSi-1 was studied by immunofluorescence. A polyclonal antibody was raised in rabbits against a fusion protein containing a 53-amino-acid polypeptide specific for the NaSi-1 sequence. The anti-NaSi-1 polyclonal antibody specifically detected a 68-kDa protein on Western blots and, by immunofluorescence specific staining, was observed in MDCK cells transfected with the NaSi-1 cotransporter. Using rat kidney cortex slices specific NaSi-1-related immunoreactivity was detected in proximal tubules and was restricted to the apical membrane. No immunoreactivity was observed in the other nephron segments. This was confirmed by Western blot analysis using proximal tubular apical and basolateral membranes isolated by free-flow electrophoresis. The results indicate that the Na/SO4-cotransporter NaSi-1 is expressed in the apical membrane of proximal tubular cells and is therefore likely to be involved in proximal reabsorption of sulphate