Three-Dimensional Imaging in Orthodontics

Orthodontic records are one of the main milestones in orthodontic therapy. Records are essential not only for diagnosis and treatment planning but also for follow-up of the case, communicating with colleagues, and evaluating the treatment outcomes. Recently, two-dimensional (2D) imaging technology, such as cephalometric and panoramic radiographs and photographs, and plaster models were routinely used. However, after the introduction of three-dimensional (3D) technologies (laser scanner, stereophotogrammetry, and computed tomography) into dentistry, 3D imaging systems are more and more commonly preferred than 2D, especially in cases with craniofacial deformities. In fact, 3D imaging provided more detailed and realistic diagnostic information about the craniofacial hard as well as soft tissue and allowed to perform easier, faster, and more reliable 3D analyses. The purpose of this review is to provide an overview of the 3D imaging techniques, including their advantages and disadvantages, and to outline the indications for 3D imaging

Ege Bölgesinde Organik Zeytin Yetiştiriciliği

Bu çalışmada, insan beslenmesi ve sağlığı açısından çok önemli bir ürün olan zeytinin yetiştirilmesinde, konvansiyonel yöntemlere alternatif olarak organik tarım yöntemlerinin uygulanabilirliğinin belirlenmesi, fenolojik, pomolojik ve ekonomik farklılıkların ortaya konması amaçlanmıştır. Çalışma Güney Ege Bölgesinin en önemli yağlık çeşidi olan Memecik zeytin çeşidinde Zeytincilik Araştırma Enstitüsünün Kemalpaşa üretim alanında 2004-2007 yılları arasında yürütülmüştür. Çalışmada organik parsellerde toprak verimliliğini artırmak amacıyla yeşil gübreleme, organik gübre, organik tarım yönetmeliğinde izin verilen mineral maddeler, konvansiyonel parsellerde ise kimyasal gübreler uygulanmıştır. Zeytin sineği popülasyon takibi Mc phail ve sarı yapışkan tuzaklar, zeytin güvesi ise delta tipi feremon tuzaklar ile yapılmıştır. Organik parsellerde zeytin sineği mücadelesinde Ecotrap, neemazal ve kaolin uygulamaları yapılmıştır. Konvansiyonel parsellerde mücadele Fenthion ile yapılmıştır. Her iki parseldeki ağaçlarda sürgün boyu, somak ve çiçek adeti, meyvelerde ise tane adedi ve ağırlığı, eni, boyu, et/çekirdek oranları, ürün miktarları tespit edilmiştir. Elde edilen zeytinyağlarında ise yağ asitleri bileşimleri ve zeytinyağı kalite parametreleri değerlendirilmiştir. Ayrıca yaprak ve toprak analizleri ile bitki besin maddelerinin değişimleri incelenmiş, parsellerden elde edilen meyvelerde kalıntı analizleri yapılmıştır. Meyve örneklerinde yapılan analizlerde organik fosforlu nitrojenli ve sülfürlü pestisitlere rastlanmamıştır. Her iki grupta da ürün miktarı, yağ kalite parametreleri ve yağ asitleri bileşiminde önemli bir farklılık bulunmamıştır. Yapılan organik tarım uygulamalarıyla, konvansiyonel yöntemler uygulanarak sağlanan verim ve kalitede ürün elde edilmiştir

The Effects of Piroxicam and Deracoxib on Canine Mammary Tumour Cell Line

Cyclooxygenase (COX) inhibitors, already widely used for the treatment of pain and inflammation, are considered as promising compounds for the prevention and treatment of neoplasia. The aim of our study was to determine the direct antiproliferative effects of nonsteroidal anti-inflammatory drugs (NSAIDs), piroxicam and deracoxib, at a variety of concentrations as both single and combined treatments on canine mammary carcinoma cell line CMT-U27 and to understand the mechanisms of cell death. MTT assay was performed to determine cell viability, and flow cytometric analyses were performed to evaluate apoptosis and cell cycle alterations. Significant decrease in cell viability was observed at high concentrations of piroxicam and deracoxib in both single and combined treatments after 72 h incubation. Combined treatment produced a significantly greater inhibition than that caused by either agent alone. Also apoptotic cell number was increased by both drugs at the cytotoxic concentrations. However, concomitant treatment of cells with piroxicam and deracoxib resulted in significant induction of apoptosis at lower concentrations and accumulation of cells in the G(0)/G(1) phase. Significant cytotoxic effects exhibited by the combination of piroxicam and deracoxib against canine mammary carcinoma cells in vitro suggest an attractive approach for the treatment of canine mammary carcinoma

The Effects of Piroxicam and Deracoxib on Canine Mammary Tumour Cell Line

Cyclooxygenase (COX) inhibitors, already widely used for the treatment of pain and inflammation, are considered as promising compounds for the prevention and treatment of neoplasia. The aim of our study was to determine the direct antiproliferative effects of nonsteroidal anti-inflammatory drugs (NSAIDs), piroxicam and deracoxib, at a variety of concentrations as both single and combined treatments on canine mammary carcinoma cell line CMT-U27 and to understand the mechanisms of cell death. MTT assay was performed to determine cell viability, and flow cytometric analyses were performed to evaluate apoptosis and cell cycle alterations. Significant decrease in cell viability was observed at high concentrations of piroxicam and deracoxib in both single and combined treatments after 72 h incubation. Combined treatment produced a significantly greater inhibition than that caused by either agent alone. Also apoptotic cell number was increased by both drugs at the cytotoxic concentrations. However, concomitant treatment of cells with piroxicam and deracoxib resulted in significant induction of apoptosis at lower concentrations and accumulation of cells in the G0/G1 phase. Significant cytotoxic effects exhibited by the combination of piroxicam and deracoxib against canine mammary carcinoma cells in vitro suggest an attractive approach for the treatment of canine mammary carcinoma

Synergistic growth inhibitory effect of deracoxib with doxorubicin against a canine mammary tumor cell line, CMT-U27

Cyclooxygenase (COX) inhibitors have been shown to exert anti-angiogenic and anti-tumor activities on many types of malignant tumors. These anticancer properties make it worthwhile to examine the possible benefit of combining COX inhibitors with other anti-cancer agents. In the present study, we evaluated the potential of deracoxib (DER) in potentiating antitumor activity of doxorubicin (DOX) in canine mammary carcinoma cells (CMT-U27). DER (50–250 µM) enhanced the antiproliferative activity of DOX by reducing the IC(50) (approximately 3- to 3.5 fold). Interaction analysis of the data showed that combinations of DOX at 0.9 µM with DER (100–250 µM) produced synergism in the CMT-U27 cell line, with a ratio index ranging from 1.98 to 2.33. In additional studies identifying the mechanism of observed synergistic effect, we found that DER strongly potentiated DOX-caused G(0)/G(1) arrest in cell cycle progression. Also, DER (100–250 µM) augmented apoptosis induction with approximately 1.35- and 1.37- fold increases in apoptotic response caused by DOX in the cells. DER enhanced the antiproliferative effect of DOX in conjunction with induction of apoptosis by modulation of Bcl-2 expression and changes in the cell cycle of the CMT-U27 cell line. Although the exact molecular mechanism of the alterations in the cell cycle and apoptosis observed with DER and DOX combinations require further investigations, the results suggest that the synergistic effect of DOX and DER combinations in CMT therapy may be achieved at relatively lower doses of DOX with lesser side effects. Therefore, combining DER with DOX may prove beneficial in the clinical treatment of canine mammary cancer

Synergistic growth inhibitory effect of deracoxib with doxorubicin against a canine mammary tumor cell line, CMT-U27

Cyclooxygenase (COX) inhibitors have been shown to exert anti-angiogenic and anti-tumor activities on many types of malignant tumors. These anticancer properties make it worthwhile to examine the possible benefit of combining COX inhibitors with other anti-cancer agents. In the present study, we evaluated the potential of deracoxib (DER) in potentiating antitumor activity of doxorubicin (DOX) in canine mammary carcinoma cells (CMT-U27). DER (50-250 mu M) enhanced the antiproliferative activity of DOX by reducing the IC50 (approximately 3- to 3.5 fold). Interaction analysis of the data showed that combinations of DOX at 0.9 mu M with DER (100-250 mu M) produced synergism in the CMT-U27 cell line, with a ratio index ranging from 1.98 to 2.33. In additional studies identifying the mechanism of observed synergistic effect, we found that DER strongly potentiated DOX-caused G(0)/G(1) arrest in cell cycle progression. Also, DER (100-250 mu M) augmented apoptosis induction with approximately 1.35- and 1.37-fold increases in apoptotic response caused by DOX in the cells. DER enhanced the antiproliferative effect of DOX in conjunction with induction of apoptosis by modulation of Bcl-2 expression and changes in the cell cycle of the CMT-U27 cell line. Although the exact molecular mechanism of the alterations in the cell cycle and apoptosis observed with DER and DOX combinations require further investigations, the results suggest that the synergistic effect of DOX and DER combinations in CMT therapy may be achieved at relatively lower doses of DOX with lesser side effects. Therefore, combining DER with DOX may prove beneficial in the clinical treatment of canine mammary cancer