Immunohistochemical evaluation of the distribution of laminin, fibronectin and type IV collagen in sciatic nerves of streptozotocine-induced diabetic rats

Amaç: Bu çalışmada, diyabetik sıçanlarda periferik sinir hücre dışı matriks proteinlerinin (laminin, fibronektin, tipIV kollajen) dağılımlarını immunohistokimyasal yöntemlerle inceledik. Gereç ve Yöntem: 250 - 300 g ağırlığında, erişkin erkek Wistar Albino sıçanlardan kontrol (n=10) ve diyabetik (n=10) gruplar oluşturuldu. Alınan sinir dokularına fibronektin, laminin ve tip IV kollajen için immunohistokimyasal inceleme yapıldı. Bulgular: Fibronektin ve laminin tutulumunun kontrol grubuna oranla artığını, tutulumların özellikle perinöryumda, Schwann hücre ve endonöral kân damarları çevresinde yoğunlaştığı izlendi. Fibronektin tutulumu perinöryumun en iç katında çok zayıfken, laminin tutulumu özellikle burada çok artmıştı. Ayrıca Tip IV kollajen tutulumunun da aynı alanlarda daha fazla olduğu ama kontrollerle arasında belirgin bir farklılık olmadığı görüldü. Sonuç: Bu bulguların ışığında diyabetin hücre dışı matriks bileşenlerinin ekspresyonunu arttırabileceği ama diğer yandan diyabetin bu bileşenlerin yapısınıbozarak immunohistokimyasal çalışmalarda antikor tutulumunu engelleyebileceği düşünülmektedir.Immunohistochemical evaluation of the distribution of laminin, fibronectin and type IV collagen in sciatic nerves of Streptozotocine-induced diabetic rats Objective: The immunohistochemical distribution of type IV collagen, fibronectin and laminin in peripheral nerves of STZ diabetic rats were studied in the present study. Methods: 2 groups were formed as control and diabetic with 20 Wistar Albino rats (10 rats in each) weighing 250 - 300 g. Sciatic nerves were excised and stained immunohistochemically with laminin, fibronectin and type IV collagen antibodies. Results: We found that increased deposition of type IV collagen, fibronectin and laminin was observed in the perineurium, and endoneurium surrounding microvessels and Schwann cells. Fibronectin and laminin immune reactions were slightly stronger in diabetic nerves. Laminin expression was most evident in the innermost layers of perineurium. But interestingly, there was almost no fibronectin expression in the same layers. Type IV collagen expression in diabetics didn't obviously differ from that in controls. Conclusion: We concluded that diabetes mellitus might increase the expression of extracellular matrixcomponents. But diabetes could also destroy the structure of these proteins and prevent them from binding immunohistochemical antibodies leading to diminished staining

Acute Transverse Myelitis With Tertiary Spinal Syphilis: Case Report

Scientific BACKGROUND: Syphilis is an systemic infection that can effect the central nervous system in all stages. Without treatment, it reaches the primary, secondary and tertiary stages, it also affects heart, central nervous system and other organs. OBJECTIVE: With the introduction of penicillin, effects of syphilis on central nervous system are decreased. Spinal cord involvement is very rare manifestation of syphilis. Syphilis should be kept in mind in transverse myelitis cases that presented with acute paraparesis. CASE: A 61 years old man presented with acute paraparesis, hyperestesia below level of T9 dermatom, fecal and uriner retantion. CONCLUSION: Our case is presented due to rare manifestation of syphilis like spinal myelitis with acute paraparesia and concomitant cardiac, cerebral, renal, aortic and retinal involvement by syphili

Effect of gene polymorphisms in transmembrane protein 18 (TMEM18) and neuronal growth regulator 1 (NEGR1) on body mass index in obese subjects

Obesity is a complex disorder with nearly epidemic proportions in many parts of the world. Genome-wide association studies have demonstrated high heritability for obesity and body mass, with associations of certain candidate genes and their variations with respect to race, geographical location/country of origin. However, the functional mechanisms and different ethnic data of these loci are still poorly understood. In this case-control study, we investigated two single nucleotide polymorphisms, rs2815752 in the neuronal growth regulator 1 (NEGR1) gene and rs6548238 in the transmembrane protein 18 (TMEM18) gene, for association in a group of obese residents of Afyonkarahisar province (Turkey). Polymorphisms were genotyped in 172 obese subjects and 77 healthy controls. The results showed no significant differences between the obese subjects and the controls in terms of the allele and genotype frequencies of the NEGR1 gene rs2815752 and the TMEM18 gene rs6548238 polymorphisms. There were no significant associations of the rs2815752 polymorphism in obese subjects and controls with regard to anthropometric measurements and body composition parameters. However, several significant associations were found for the rs6548238 polymorphism with regard to anthropometric measurements and body composition. Consequently, there were no significant differences between the genotype and allele frequencies of NEGR1 gene rs2815752 and TMEM18 gene rs6548238 polymorphisms in the obese group and the controls. There were significant associations for the rs6548238 polymorphism, but not the rs2815752 polymorphism, with the anthropometric measurements and body composition parameters in the group of obese subjects