11 research outputs found
Selectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDD Cis-Acting elements in Transgenic Plants
No full textExcision of a selectable marker in transgenic rice (Oryza sativa L.) using a chemically regulated Cre/loxP system
No full text10.1007/s00299-004-0909-5Plant Cell Reports24286-94PCRP
Restoring pollen fertility in transgenic male-sterile eggplant by Cre/loxp-mediated site-specific recombination system
No full textImprovement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
No full textExpression of active Streptomyces phage phiC31 integrase in transgenic wheat plants
No full textSite-specific recombination systems are becoming an important tool for the genetic modification of crop plants. Here we report the functional expression of the Streptomyces phage-derived phiC31 recombinase (integrase) in wheat. T-DNA constructs containing a phiC31 integrase transgene were stably transformed into wheat plants via particle gun bombardment. A plant-virus-based assay system was used to monitor the site-specific recombination activity of the recombinant integrase protein in vivo. We established several independent doubled haploid (DH) inbred lines that constitutively express an active integrase enzyme without any apparent detrimental effects on plant growth and development. The potential of phiC31 integrase expression in crop plants related to transgene control technologies or hybrid breeding systems is discussed.Myroslava Rubtsova, Katja Kempe, Angelika Gils, Ainur Ismagul, Jens Weyen and Mario Gils
Functionality of the beta/six site-specific recombination system in tobacco and Arabidopsis: A novel tool for genetic engineering of plant genomes
No full textGronlund JT, Stemmer C, Lichota J, Merkle T, Grasser KD. Functionality of the beta/six site-specific recombination system in tobacco and Arabidopsis: A novel tool for genetic engineering of plant genomes. Plant Molecular Biology. 2007;63(4):545-556
The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy
No full textGenerating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System
No full textLevel of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.)
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