78 research outputs found
Crystallization and preliminary X-ray crystallographic analysis of SEDL
SEDL (known also as sedlin) is a 140 amino-acid protein with a putative role in endoplasmic reticulum-to-Golgi transport. Several missense mutations and deletion mutations in the SEDL gene, which result in protein truncation by frame shift, are responsible for spondyloepiphyseal dysplasia tarda, a progressive skeletal disorder. The protein is identical to MIP-2A, which was shown to interact physically with c-myc promotor-binding protein 1 (MBP-1) and relieve the regulatory role of MBP-1 as a general transcription repressor. In order to gain insights into the function of SEDL by structural analysis, the protein was overexpressed and crystallized as a first step. SEDL was overexpressed in Escherichia coli and crystallized using the hanging-drop vapour-diffusion method at 298 K. The crystals belong to the orthorhombic space group C2221, with unit-cell parameters a = 46.69, b = 101.30, c = 66.15 A. The unit cell is likely to contain one molecule of SEDL, with a crystal volume per protein mass (VM) of 2.36 A3 Da-1 and a solvent content of about 47.9% by volume. A native data set to 2.8 A resolution was obtained from a flash-cooled crystal using synchrotron radiation.open1
A New Technique for Reachability of States in Concatenation Automata
We present a new technique for demonstrating the reachability of states in
deterministic finite automata representing the concatenation of two languages.
Such demonstrations are a necessary step in establishing the state complexity
of the concatenation of two languages, and thus in establishing the state
complexity of concatenation as an operation. Typically, ad-hoc induction
arguments are used to show particular states are reachable in concatenation
automata. We prove some results that seem to capture the essence of many of
these induction arguments. Using these results, reachability proofs in
concatenation automata can often be done more simply and without using
induction directly.Comment: 23 pages, 1 table. Added missing affiliation/funding informatio
Bond-length dependence of charge-transfer excitations and stretch phonon modes in perovskite ruthenates: Evidence of strong p – d hybridization effects
We reported the optical conductivity spectra of the Ruddlesden-Popper series ruthenates, i.e., Srn+1RunO3n+1
and Can+1RunO3n+1, where n=1, 2, and `. Among various optical transitions, we investigated two Ru-O
related modes, i.e., the charge-transfer excitation and the transverse stretching phonon. We found that their
frequency shifts are not much affected by a structural dimensionality, but are closely related to the Ru-O bond
length. Through the quantitative analysis of the charge-transfer excitation energy, we could demonstrate that
the p–d hybridization should play an important role in determining their electronic structure. In addition, we
discussed how the electronic excitation could contribute the lattice dynamics in the metallic ruthenate
11 nm hard X-ray focus from a large-aperture multilayer Laue lens
The focusing performance of a multilayer Laue lens (MLL) with 43.4 μm aperture, 4 nm finest zone width and 4.2 mm focal length at 12 keV was characterized with X-rays using ptychography method. The reconstructed probe shows a full-width-at-half-maximum (FWHM) peak size of 11.2 nm. The obtained X-ray wavefront shows excellent agreement with the dynamical calculations, exhibiting aberrations less than 0.3 wave period, which ensures the MLL capable of producing a diffraction-limited focus while offering a sufficient working distance. This achievement opens up opportunities of incorporating a variety of in-situ experiments into ultra high-resolution X-ray microscopy studies
Deltamethrin residues applied in different formulations in staked cucumber and the actions of insecticides on the pickleworm control
HIF-1 activation induces doxorubicin resistance in MCF7 3-D spheroids via P-glycoprotein expression: a potential model of the chemo-resistance of invasive micropapillary carcinoma of the breast
BACKGROUND: Invasive micropapillary carcinoma (IMPC) of the breast is a distinct and aggressive variant of luminal type B breast cancer that does not respond to neoadjuvant chemotherapy. It is characterized by small pseudopapillary clusters of cancer cells with inverted cell polarity. To investigate whether hypoxia-inducible factor-1 (HIF-1) activation may be related to the drug resistance described in this tumor, we used MCF7 cancer cells cultured as 3-D spheroids, which morphologically simulate IMPC cell clusters. METHODS: HIF-1 activation was measured by EMSA and ELISA in MCF7 3-D spheroids and MCF7 monolayers. Binding of HIF-1α to MDR-1 gene promoter and modulation of P-glycoprotein (Pgp) expression was evaluated by ChIP assay and FACS analysis, respectively. Intracellular doxorubicin retention was measured by spectrofluorimetric assay and drug cytotoxicity by annexin V-FITC measurement and caspase activity assay. RESULTS: In MCF7 3-D spheroids HIF-1 was activated and recruited to participate to the transcriptional activity of MDR-1 gene, coding for Pgp. In addition, Pgp expression on the surface of cells obtained from 3-D spheroids was increased. MCF7 3-D spheroids accumulate less doxorubicin and are less sensitive to its cytotoxic effects than MCF7 cells cultured as monolayer. Finally, HIF-1α inhibition either by incubating cells with 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (a widely used HIF-1α inhibitor) or by transfecting cells with specific siRNA for HIF-1α significantly decreased the expression of Pgp on the surface of cells and increased the intracellular doxorubicin accumulation in MCF7 3-D spheroids. CONCLUSIONS: MCF7 breast cancer cells cultured as 3-D spheroids are resistant to doxorubicin and this resistance is associated with an increased Pgp expression in the plasma membrane via activation of HIF-1. The same mechanism may be suggested for IMPC drug resistance
Effective and safe proton pump inhibitor therapy in acid-related diseases – A position paper addressing benefits and potential harms of acid suppression
Discrete Choice Experiments in Developing Countries: Willingness to Pay Versus Willingness to Work
Andrographolide induces Nrf2 and heme oxygenase 1 in astrocytes by activating p38 MAPK and ERK
Model-based adaptive phase I trial design of post-transplant decitabine maintenance in myelodysplastic syndrome
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