8 research outputs found
In vitro antioxidant and anticancer activity of young Zingiber officinale against human breast carcinoma cell lines
<p>Abstract</p> <p>Background</p> <p>Ginger is one of the most important spice crops and traditionally has been used as medicinal plant in Bangladesh. The present work is aimed to find out antioxidant and anticancer activities of two Bangladeshi ginger varieties (Fulbaria and Syedpuri) at young age grown under ambient (400 μmol/mol) and elevated (800 μmol/mol) CO<sub>2 </sub>concentrations against two human breast cancer cell lines (MCF-7 and MDA-MB-231).</p> <p>Methods</p> <p>The effects of ginger on MCF-7 and MDA-MB-231 cell lines were determined using TBA (thiobarbituric acid) and MTT [3-(4,5-dimethylthiazolyl)-2,5-diphenyl-tetrazolium bromide] assays. Reversed-phase HPLC was used to assay flavonoids composition among Fulbaria and Syedpuri ginger varieties grown under increasing CO<sub>2 </sub>concentration from 400 to 800 μmol/mol.</p> <p>Results</p> <p>Antioxidant activities in both varieties found increased significantly (P ≤ 0.05) with increasing CO<sub>2 </sub>concentration from 400 to 800 μmol/mol. High antioxidant activities were observed in the rhizomes of Syedpuri grown under elevated CO<sub>2 </sub>concentration. The results showed that enriched ginger extract (rhizomes) exhibited the highest anticancer activity on MCF-7 cancer cells with IC<sub>50 </sub>values of 34.8 and 25.7 μg/ml for Fulbaria and Syedpuri respectively. IC<sub>50 </sub>values for MDA-MB-231 exhibition were 32.53 and 30.20 μg/ml for rhizomes extract of Fulbaria and Syedpuri accordingly.</p> <p>Conclusions</p> <p>Fulbaria and Syedpuri possess antioxidant and anticancer properties especially when grown under elevated CO<sub>2 </sub>concentration. The use of ginger grown under elevated CO<sub>2 </sub>concentration may have potential in the treatment and prevention of cancer.</p
Repellent and mosquitocidal effects of leaf extracts of Clausena anisata against the Aedes aegypti mosquito (Diptera: Culicidae)
Mosquitoes are rapidly developing resistance to insecticides
that millions of people relied on to protect themselves
from the diseases they carry, thereby creating a need to
develop new insecticides. Clausena anisata is used traditionally
as an insect repellent by various communities in Africa
and Asia. For this study, the repellency and adulticidal activities
of leaf extracts and compounds isolated from this plant
species were evaluated against the yellow fever mosquito,
Aedes aegypti. In the topical application assays, using total
bites as an indicator, repellency was dose dependent, with
the acetone crude extract (15 %) having 93 % repellence and
the hexane fraction (7.5 %) 67 % repellence after 3 h.
Fractionation resulted in a loss of total repellence. As
mosquito-net treating agents, the acetone and hexane extracts
of C. anisata, both at 15 %, had average repellences of 46.89
± 2.95 and 50.13 ± 2.02 %, respectively, 3 h after exposure.
The C. anisata acetone extract and its hexane fraction caused
mosquito knockdown and eventually death when nebulised
into the testing chamber, with an EC50 of 78.9 mg/ml (7.89 %) and 71.6 mg/ml (7.16 %) in the first 15 min after
spraying. C. anisata leaf extracts have potential to be included
in protection products against mosquitoes due to the repellent
and cidal compounds contained therein.The University of Pretoria and the National Research Foundation.http://link.springer.com/journal/113562017-06-30hb2016Paraclinical Science