Regulation of DNA synthesis and the cell cycle in human prostate cancer cells and lymphocytes by ovine uterine serpin

<p>Abstract</p> <p>Background</p> <p>Uterine serpins are members of the serine proteinase inhibitor superfamily. Like some other serpins, these proteins do not appear to be functional proteinase inhibitors. The most studied member of the group, ovine uterine serpin (OvUS), inhibits proliferation of several cell types including activated lymphocytes, bovine preimplantation embryos, and cell lines for lymphoma, canine primary osteosarcoma and human prostate cancer (PC-3) cells. The goal for the present study was to evaluate the mechanism by which OvUS inhibits cell proliferation. In particular, it was tested whether inhibition of DNA synthesis in PC-3 cells involves cytotoxic actions of OvUS or the induction of apoptosis. The effect of OvUS in the production of the autocrine and angiogenic cytokine interleukin (IL)-8 by PC-3 cells was also determined. Finally, it was tested whether OvUS blocks specific steps in the cell cycle using both PC-3 cells and lymphocytes.</p> <p>Results</p> <p>Recombinant OvUS blocked proliferation of PC-3 cells at concentrations as low as 8 μg/ml as determined by measurements of [³H]thymidine incorporation or ATP content per well. Treatment of PC-3 cells with OvUS did not cause cytotoxicity or apoptosis or alter interleukin-8 secretion into medium. Results from flow cytometry experiments showed that OvUS blocked the entry of PC-3 cells into S phase and the exit from G₂/M phase. In addition, OvUS blocked entry of lymphocytes into S phase following activation of proliferation with phytohemagglutinin.</p> <p>Conclusion</p> <p>Results indicate that OvUS acts to block cell proliferation through disruption of the cell cycle dynamics rather than induction of cytotoxicity or apoptosis. The finding that OvUS can regulate cell proliferation makes this one of only a few serpins that function to inhibit cell growth.</p

Reducing ultraviolet radiation exposure among outdoor workers: State of the evidence and recommendations

<p>Abstract</p> <p>Objective</p> <p>Outdoor workers have high levels of exposure to ultraviolet radiation and the associated increased risk of skin cancer. This paper describes a review of: 1) descriptive data about outdoor workers' sun exposure and protection and related knowledge, attitudes, and policies and 2) evidence about the effectiveness of skin cancer prevention interventions in outdoor workplaces.</p> <p>Data sources</p> <p>Systematic evidence-based review.</p> <p>Data synthesis</p> <p>We found variable preventive practices, with men more likely to wear hats and protective clothing and women more likely to use sunscreen. Few data document education and prevention policies.</p> <p>Conclusion</p> <p>Reports of interventions to promote sun-safe practices and environments provide encouraging results, but yield insufficient evidence to recommend current strategies as effective. Additional efforts should focus on increasing sun protection policies and education programs in workplaces and evaluating whether they improve the health behavior of outdoor workers.</p

Retention on Buprenorphine Is Associated with High Levels of Maximal Viral Suppression among HIV-Infected Opioid Dependent Released Prisoners

HIV-infected prisoners lose viral suppression within the 12 weeks after release to the community. This prospective study evaluates the use of buprenorphine/naloxone (BPN/NLX) as a method to reduce relapse to opioid use and sustain viral suppression among released HIV-infected prisoners meeting criteria for opioid dependence (OD).From 2005-2010, 94 subjects meeting DSM-IV criteria for OD were recruited from a 24-week prospective trial of directly administered antiretroviral therapy (DAART) for released HIV-infected prisoners; 50 (53%) selected BPN/NLX and were eligible to receive it for 6 months; the remaining 44 (47%) selected no BPN/NLX therapy. Maximum viral suppression (MVS), defined as HIV-1 RNA<50 copies/mL, was compared for the BPN/NLX and non-BPN/NLX (N = 44) groups.The two groups were similar, except the BPN/NLX group was significantly more likely to be Hispanic (56.0% v 20.4%), from Hartford (74.4% v 47.7%) and have higher mean global health quality of life indicator scores (54.18 v 51.40). MVS after 24 weeks of being released was statistically correlated with 24-week retention on BPN/NLX [AOR = 5.37 (1.15, 25.1)], having MVS at the time of prison-release [AOR = 10.5 (3.21, 34.1)] and negatively with being Black [AOR = 0.13 (0.03, 0.68)]. Receiving DAART or methadone did not correlate with MVS.In recognition that OD is a chronic relapsing disease, strategies that initiate and retain HIV-infected prisoners with OD on BPN/NLX is an important strategy for improving HIV treatment outcomes as a community transition strategy

Ecuadom hogares inteligentes

ECUADOM ha sido creada para ofrecer a sus clientes Sistemas Domóticos y brindar varios servicios que se puedan agrupar en cuatro aspectos o ámbitos principales, ahorro energético, protección patrimonial, confort y comunicaciones

Quiltour s.a.-operadora turistica

Quiltour S.A. se establecerá en la ciudad de Guayaquil – Ecuador, con el propósito de prestar servicios turísticos en actividades de Operadora de Turismo la que le ofertará paquetes turísticos locales y nacionales. Por consiguiente para hacer realidad nuestra propuesta contaremos con expertos profesionales que desarrollaran paquetes turísticos con criterio técnico

Assessment of visual impact due to surface mining with the Lvi method

Surface mining and quarrying typically generate a number of environmental concerns among which landscape alteration is one of the most significant. Although landscape and visual impact does not directly affect public health, it usually generates a negative reaction among potential observers, sometimes influencing the socioeconomic development of the surrounding impact territory. The landscape and visual impact assessment involves individual perceptions, aesthetic tastes and visual comprehension; however, some aspects of landscape modification can be objectively measured in order to quantify the magnitude of change. The article summarizes the results of previous studies focused on the implementation of the Lvi (Level of Visual Impact) method to a variety of cases within the European territory and discusses the adequacy of the method in providing an objective estimation of the visual damage produced by surface mining and quarrying. The visual impact indicator Lvi takes into account two parameters among those physically measurable: the extent of the visible alteration and the chromatic contrast between the color of the bare rock and that of the surrounding landscape. Both parameters can be quantified by processing one or more digital images taken from the most significant viewpoints (public roads, towns, villages, touristic sites, etc.). The article also includes the results of a recent study where the use of a global impact factor LVI to be associated to a given route has been proposed, in order to account for the variability of the visual impact along the route. Other studies proved the visual impact indicator Lvi to be significantly correlated with the subjective perception of potential observers, the results of a test carried out in the University of Cagliari are briefly reported in this article. Some critical aspects regarding the variability of the results on the basis of the shooting conditions (type of camera, season and time of the day, etc.) are discussed, as well as the possibility of developing the assessment procedure as to include the estimate of the ante-operam quality of the impact territory under exam and the number and type of people who are likely to observe the landscape modificatio