The creative potential of museum pedagogy within the modern society

The paper explores the pedagogical and culturaleducational significance of museums, describes their historical evolution, and defines their positive influence on the level of the people's culture and education. The aim of this research is to study the formation of a human individual's cultural and educational potential by means of Museum Pedagogy. The research shows that the methodological foundations of Museum Pedagogy development harmonically unite the fundamental principles of educational philosophy and the specificity of the museum-educational space. It is also defined that Museum Pedagogy as a scientific discipline is designed concerning the peculiarities of the modern museum, where the unique object-space environment is being formed, on the basis of the open cultural-civilizational dialogue and interaction with the objects of Spiritual Heritage. Therefore, the acquisition and comprehension of new mind-challenging ideas of the surrounding reality lead to certain stimulating, correctional, rehabilitation and recreational effects. © 2017. revistaESPACIOS.com

THE IMPACT OF CONSTRUCTION INDUSTRY ON THE CONTENT OF HEAVY METALS IN THE AIR ENVIRONMENT OF KRASNOYARSK CITY

Proceedings of the XXV ISUF International Conference “Urban Form and Social Context: from Traditions to Newest Demands” (Krasnoyarsk, July 5–9, 2018)The construction industry has a significant role in the environmental situation of the city of Krasnoyarsk providing the population with residential, industrial and road construction facilities. A necessary component of construction activity is the enterprises for the production of building materials using a wide range of chemical compounds that annually enter the atmosphere, water bodies, groundwater, soil in the form of industrial emissions, affecting the environmental situation. The construction industry is also impossible to imagine without the use of vehicles, the operation of which is impossible without fuel that absorbs oxygen and emits exhaust gases containing heavy metals in their composition. From the environment dangerous substances enter the human body through the skin, inhaled air, food and eventually cause its functional changes. Exceeding the maximum permissible concentrations heavy metals can have a toxic effect on the human body, so it is necessary to measure their concentration in the air of Krasnoyarsk city regularly. The most common heavy metals entering the atmosphere as a result of industrial emissions include lead, mercury, cadmium, zinc. Lead has a toxic effect, it accumulates in kidneys, liver and other vital organs, gradually leading to disruption of the entire body functioning. Mercury has a toxic effect on the human body, animals and natural components; it is widely used in various industries and a part of a number of pesticides. Cadmium is a chemical element with toxic properties exceeding the properties of lead. It easily passes from soil to plants. Zinc is widely used in the production of paint products, medicine and printing. In case of excessive intake by the body it has a carcinogenic effect

The creative potential of museum pedagogy within the modern society

The paper explores the pedagogical and culturaleducational significance of museums, describes their historical evolution, and defines their positive influence on the level of the people's culture and education. The aim of this research is to study the formation of a human individual's cultural and educational potential by means of Museum Pedagogy. The research shows that the methodological foundations of Museum Pedagogy development harmonically unite the fundamental principles of educational philosophy and the specificity of the museum-educational space. It is also defined that Museum Pedagogy as a scientific discipline is designed concerning the peculiarities of the modern museum, where the unique object-space environment is being formed, on the basis of the open cultural-civilizational dialogue and interaction with the objects of Spiritual Heritage. Therefore, the acquisition and comprehension of new mind-challenging ideas of the surrounding reality lead to certain stimulating, correctional, rehabilitation and recreational effects. © 2017. revistaESPACIOS.com

Expression of Metalloproteinases and Type I and III Collagens during Healing of Excisional Skin Wound on the Abdomen and Back in Rats

The study was carried out using a novel rat model developed in our laboratory, namely16 mm diameter circular excisional wounds were generated on the abdomen which resulted in minimal scarring. Restoration of the skin integrity was completed by day 60 after the wounding surgery. By this time, regenerates on the abdomen were stronger than on the back (at, respectively, 58 and 17.4 % of the tensile strength of the intact skin at corresponding location) and the ratio of type I and type III collagens in regenerates on the abdomen reached the level of intact skin at the same location. On days 3 to 14, the ratio of Mmp9/Timp1 expression levels on the abdomen was higher than on the back. On days 20 and 30, the Mmp9/Timp1 ratio on the abdomen was identical to the level of intact skin, whereas the increased MMPs expression levels on the back were maintained until day 30. It has been shown for the first time that according to functional and molecular characteristics, wound healing on the abdomen of an adult rat is more similar to complete regeneration than scarring repair of the back skin. © 2020, Springer Science+Business Media, LLC, part of Springer Nature

Экспрессия металлопротеиназ и коллагенов I и III типов при заживлении кожной раны на животе и спине крыс

The study was carried out using a novel rat model developed in our laboratory, namely16 mm diameter circular excisional wounds were generated on the abdomen which resulted in minimal scarring. Restoration of the skin integrity was completed by day 60 after the wounding surgery. By this time, regenerates on the abdomen were stronger than on the back (at, respectively, 58 % and 17.4 % of the tensile strength of the intact skin at corresponding location) and the ratio of type I and type III collagens in regenerates on the abdomen reached the level of intact skin at the same location. On days 3 to 14, the ratio of Mmp9/Timp1 expression levels on the abdomen was higher than on the back. On days 20 and 30, the Mmp9/Timp1 ratio on the abdomen was identical to the level of intact skin, whereas the increased metalloproteinase expression levels on the back were maintained until day 30. It has been shown for the first time that according to functional and molecular characteristics, wound healing on the abdomen of an adult rat is more similar to complete regeneration than scarring repair of the back skin.Исследование проведено на новой разработанной нами модели заживления кожи после эксцизионной раны диаметром 16 мм на животе крысы с образованием минимального рубца в сравнении с общеизвестной моделью раны на спине. Показано восстановление прочностных характеристик кожи к 60-м суткам после повреждения: на животе регенерат прочнее (58% прочности на разрыв от интактного контроля), чем на спине (17.4% от контроля), а соотношение коллагена I и коллагена III на животе достигает уровня в интактной коже. При этом с 3-х по 14-е сутки соотношение уровней экспрессии Mmp9 / Timp1 в коже живота было выше, чем после аналогичной травмы кожи спины, на 20-е и 30-е сутки уже не отличалось от интактной кожи, в то время как в ране спины вплоть до 30-х суток наблюдали повышенный уровень экспрессии металлопротеиназ. Показано, что заживление раны на животе взрослой крысы по функциональным и молекулярным характеристикам ближе к полноценной регенерации, чем рубцовая репарация кожи спины

Molecular mechanisms of splenectomy-induced hepatocyte proliferation

Functional and anatomical connection between the liver and the spleen is most clearly manifested in various pathological conditions of the liver (cirrhosis, hepatitis). The mechanisms of the interaction between the two organs are still poorly understood, as there have been practically no studies on the influence exerted by the spleen on the normal liver. Mature male Sprague-Dawley rats of 250-260 g body weight, 3 months old, were splenectomized. The highest numbers of Ki67+ hepatocytes in the liver of splenectomized rats were observed at 24 h after the surgery, simultaneously with the highest index of Ki67-positive hepatocytes. After surgical removal of the spleen, expression of certain genes in the liver tissues increased. A number of genes were upregulated in the liver at a single time point of 24 h, including Ccne1, Egf, Tnfa, Il6, Hgf, Met, Tgfb1r2 and Nos2. The expression of Ccnd1, Tgfb1, Tgfb1r1 and Il10 in the liver was upregulated over the course of 3 days after splenectomy. Monitoring of the liver macrophage populations in splenectomized animals revealed a statistically significant increase in the proportion of CD68-positive cells in the liver (as compared with sham-operated controls) detectable at 24 h and 48 h after the surgery. The difference in the liver content of CD68-positive cells between splenectomized and sham-operated animals evened out by day 3 after the surgery. No alterations in the liver content of CD163-positive cells were observed in the experiments. A decrease in the proportion of CD206-positive liver macrophages was observed at 48 h after splenectomy. The splenectomy-induced hepatocyte proliferation is described by us for the first time. Mechanistically, the effect is apparently induced by the removal of spleen as a major source of Tgfb1 (hepatocyte growth inhibitor) and subsequently supported by activation of proliferation factor-encoding genes in the liver. Copyright: © 2020 Elchaninov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Design, Spectral Characteristics, and Possibilities for Practical Application of BODIPY FL-Labeled Monoterpenoid

This article describes the design and biological properties of a BODIPY FL-labeled monoterpenoid BF2-meso-(4-((1″R)-6″,6″-dimethylbicyclo[3.1.1]hept-2″-ene-2″)yl-methoxycarbonylpropyl)-3,3′,5,5′-tetramethyl-2,2′-dipyrromethene conjugate (BODIPYmyrt). The fluorophore was characterized using X-ray, NMR, MS, and UV/vis spectroscopy. The conjugate exhibits a high quantum yield (to ∼100%) in the region 515-518 nm. BODIPYmyrt effectively penetrates the membranes of the bacterial and fungal cells and therefore can be used to examine the features of a broad spectrum of Gram-positive and Gram-negative bacteria and pathogenic fungi as well. Moreover, BODIPYmyrt exhibits a moderate tropism to the subcellular structures in mammalian cells (e.g., mitochondria), thereby providing an attractive scaffold for fluorophores to examine these particular organelles