Predicting and Preventing: Developing a Risk Assessment Mechanism for Residential Housing

The introduction of legislative requirements placed upon key agencies to consider the crime and disorder implications of every decision that they make has been a progressive step in crime reduction. Yet this requirement will not achieve its potential unless these partner agencies can agree upon which factors are indeed criminogenic and therefore what impact their decisions will have upon crime. Recognising the need for clarification within the field of designing out crime within the built environment, this paper presents a comprehensive, yet straightforward and usable crime risk assessment mechanism which Architectural Liaison Officers and Crime Prevention Design Advisors can use to predict the vulnerability of residential housing to future crime and disorder problems

LIM domain-binding 1 maintains the terminally differentiated state of pancreatic β cells

The recognition of β cell dedifferentiation in type 2 diabetes raises the translational relevance of mechanisms that direct and maintain β cell identity. LIM domain-binding protein 1 (LDB1) nucleates multimeric transcriptional complexes and establishes promoter-enhancer looping, thereby directing fate assignment and maturation of progenitor populations. Many terminally differentiated endocrine cell types, however, remain enriched for LDB1, but its role is unknown. Here, we have demonstrated a requirement for LDB1 in maintaining the terminally differentiated status of pancreatic β cells. Inducible ablation of LDB1 in mature β cells impaired insulin secretion and glucose homeostasis. Transcriptomic analysis of LDB1-depleted β cells revealed the collapse of the terminally differentiated gene program, indicated by a loss of β cell identity genes and induction of the endocrine progenitor factor neurogenin 3 (NEUROG3). Lineage tracing confirmed that LDB1-depleted, insulin-negative β cells express NEUROG3 but do not adopt alternate endocrine cell fates. In primary mouse islets, LDB1 and its LIM homeodomain-binding partner islet 1 (ISL1) were coenriched at chromatin sites occupied by pancreatic and duodenal homeobox 1 (PDX1), NK6 homeobox 1 (NKX6.1), forkhead box A2 (FOXA2), and NK2 homeobox 2 (NKX2.2) - factors that co-occupy active enhancers in 3D chromatin domains in human islets. Indeed, LDB1 was enriched at active enhancers in human islets. Thus, LDB1 maintains the terminally differentiated state of β cells and is a component of active enhancers in both murine and human islets

Intervalo e intensidade de desfolhação nas taxas de crescimento, senescência e desfolhação e no equilíbrio de gramíneas em associação Interval and intensity of defoliation on the growth, senescence and defoliation fluxes and equilibrium of associated grasses

O experimento foi realizado no INRA (Institut National de la Recherche Agronomique) em Theix, França. Duas gramíneas (azevém perene e festuca)foram semeadas em caixas (0,13 m²) e submetidas a três intervalos (3,5, 7 e 14 dias) e três intensidades (160, 320 e 640 bocados.m-2 a cada desfolhação) de desfolhação, com quatro repetições por tratamento. Quatro meses após a semeadura, a cada data de desfolhação, as caixas foram oferecidas, individualmente, a quatro ovelhas secas e retiradas após a realização de um determinado número de bocados. Para cada gramínea foram estudadas as taxas de crescimento, senescência e desfolhação. Os resultados mostraram que o intervalo e a intensidade de desfolhação tiveram efeitos diferenciados nas taxas de crescimento, senescência e desfolhação e no equilíbrio da associação.<br>The experiment was carried in INRA (Institut National de la Recherche Agronomique) at Theix, France. Two grasses (perennial ryegrass and tall fescue) were grown in sward boxes (0,13 m²) and submitted to three defoliation intervals (3,5, 7 and 14 days between two successive defoliation) and three defoliation intensities (160, 320 and 640 bites.m-2 at each defoliation). Four months after sowing, at defoliation date, sward boxes were offered to four individual dry ewes and removed after a given number of bites had been taken. For each grass species, the growth, senescence and defoliation fluxes were studied. The results showed that the interval and the intensity of grazing had distinct effects on the growth, senescence and defoliation fluxes as well as on the equilibrium of grass mixtures