ИСПОЛЬЗОВАНИЕ ЦВЕТОКОДИРОВАННЫХ ДОПЛЕРОГРАФИЧЕСКИХ МЕТОДИК В ДИАГНОСТИКЕ ДОБРОКАЧЕСТВЕННЫХ И ЗЛОКАЧЕСТВЕННЫХ НОВООБРАЗОВАНИЙ НИЖНИХ КОНЕЧНОСТЕЙ

The authors presented results of blood flow examination in soft and bone tissues masses of lower extremities. Qualitative and quantitative blood flow indices were studied compared and systematized using color-coded Doppler technique and spectral dopplerography.Представлены результаты исследования показателей кровотока в мягкотканных и костных новообразованиях нижних конечностей 78 пациентов. Изучены, сопоставлены и систематизированы качественные и количественные показатели кровотока с использованием цветокодированных доплеровских методик и спектральной доплерографии

Возможности ультразвуковой диагностики патологии стопы и голеностопного сустава

Ultrasonography is a useful tool for imaging, which can be used for the assessment of joints and periarticular structures. In patients with pain and/or swelling of the foot and ankle, ultrasonography provides information about the presence of joint effusion, synovitis, tendinopathy and tendons tears. Ultrasonic diagnostics of various damages both pathological conditions of the foot and ankle should become daily and widely used method, allowing to optimize diagnostics and medical tactics.Ультрасонография - полезный инструмент в отображении состояния суставов и околосуставных структур. У пациентов с болью и/или отеком стопы и голеностопного сустава ультрасонография позволяет визуализировать наличие гематомы, синовита, тендинопатии и разрывов сухожилий. Ультразвуковая диагностика различных повреждений и патологических состояний стопы и голеностопного сустава должна стать повседневно и широко используемым методом, позволяющим оптимизировать диагностику и лечебную тактику

Role for chondroitin sulfate glycosaminoglycan in NEDD9-mediated breast cancer cell growth

Item does not contain fulltextThere are lines of evidence demonstrating that NEDD9 (Cas-L, HEF-1) plays a key role in the development, progression, and metastasis of breast cancer cells. We previously reported that NEDD9 plays a critical role for promoting migration and growth of MDA-MB-231. In order to further characterize the mechanisms of NEDD9-mediated cancer migration and growth, stable cells overexpressing NEDD9 were generated using HCC38 as a parental cell line which expresses low level of endogenous NEDD9. Microarray studies demonstrated that core proteins of CD44 and Serglycin were markedly upregulated in HCC38(NEDD9) cells compared to HCC38(Vector) cells, while those of Syndecan-1, Syndecan-2, and Versican were downregulated in HCC38(NEDD9). Importantly, enzymes generating chondroitin sulfate glycosaminoglycans (CS) such as CHST11, CHST15, and CSGALNACT1 were upregulated in HCC38(NEDD9) compared to HCC38(Vector). Immunofluorescence studies using specific antibody, GD3G7, confirmed the enhanced expression of CS-E subunit in HCC38(NEDD9). Immunoprecipitation and western blotting analysis demonstrated that CS-E was attached to CD44 core protein. We demonstrated that removing CS by chondroitinase ABC significantly inhibited anchorage-independent colony formation of HCC38(NEDD9) in methylcellulose. Importantly, the fact that GD3G7 significantly inhibited colony formation of HCC38(NEDD9) cells suggests that CS-E subunit plays a key role in this process. Furthermore, treatment of HCC38(NEDD9) cells with chondroitinase ABC or GD3G7 significantly inhibited mammosphere formation. Exogenous addition of CS-E enhanced colony formation and mammosphere formation of HCC38 parental and HCC38(Vector) cells. These results suggest that NEDD9 regulates the synthesis and expression of tumor associated glycocalyx structures including CS-E, which plays a key role in promoting and regulating breast cancer progression and metastasis and possibly stem cell phenotypes