Приоритетные механизмы лекарственного обеспечения населения Российской Федерации

The main directions of modernisation of the pharmaceutical care provision of the population of the Russian Federation are systematised based on the results of the analysis of normative legal acts. В статье по результатам проведенного анализа нормативных правовых актов систематизированы основные направления модернизации лекарственного обеспечения населения Российской Федерации.

The layer by layer selective laser synthesis of ruby

In the work, features of the layer-by-layer selective laser synthesis (SLS) of ruby from an Al2O3-Cr2O3 mixture are considered depending on the irradiation power, the laser beam traverse speed, the height and amount of the backfill of powder layers. It has been established that, under irradiation, a track consisting of polycrystalline textured ruby forms. The morphology of the surface of the track and its crystalline structure are determined by the irradiation conditions

Biomechanical approaches to prevention of n0nrational "exploitation" of spine

В течение жизнедеятельности основная часть физической нагрузки приходится на позвоночник. В целом, все звенья тела включены в работу, эффективность которой зависит от суставов опорно-двигательного аппарата. Они активно участвуют в двигательных действиях различной сложности, мощности и координации. Неравномерное распределение нагрузки между мышечными группами может привести к определенным негативным последствиям. В этой связи необходим учет биомеханических подходов к процессу физического воспитания и доминирующим направлением становятся законы биомеханики(сила тяжести и силы инерции), обеспечивающих эффективность работы позвоночника в результате выполнения физических упражнений и различных видов спортивных практик.During the life the bulk of physical activity has given on the spine. In general, all parts of the body are included in the work, the effectiveness of which depends on the joints of the musculoskeletal system. They are actively involved in driving the actions of varying complexity, power and coordination. The uneven distribution of the load between the muscle groupscan lead to some negative consequences. In this regard, account must be taken to biomechanical approaches to physical education and the dominant direction is given to biomechanical laws (gravity and inertia), ensuring the effectiveness of the spine activity because of exercise and various sports practices

Sour milk beverage preparation technology

In order to expand the range of dairy products for the elderly, a technology has been developed for preparing a fermented milk drink with stevia, using a combined starter culture from kefir fungi, bulgarian bacillus, thermophilic streptococcus and bifidobacteria. During the research, modern standard methods were used. The research was carried out in the laboratory of the Research Institute of Agroecology of the Gorsk State Agrarian University, in the laboratory of food products of the commodity-technological faculty of the Gorsky State Agrarian University, in the Scientific Laboratory of Feed and Metabolism of the Stavropol State Agrarian University. In laboratory conditions, the quality of milk and fermented milk drink was determined. The organoleptic, physicochemical, microbiological indicators of the quality of milk and milk drink, the amino acid composition of proteins, the content of toxic elements, and nutritional value have been investigated. During our research, we found that the quality of the used pasteurized cow's milk meets the requirements of the standard and TR CU. During laboratory studies, it was found that the quality indicators of the fermented milk drink are within the requirements of the current NTD. According to economic calculations, the production of this product is profitable

Raman spectroscopy evaluation of Polyvinylchloride structure

Polyvinylchloride (PVC) is one of the most widely used commercial polymers, and the field of its application continues to expand rapidly. Effective development of state-of-art functional materials, such as PVC-based nanocomposites, copolymers, blends and fabrics, subjected to special kinds of treatment, requires elaboration of new, more informative approaches of PVC structure evaluation. Industrial PVC is characterized by variety of states of macromolecules and very low degree of crystallinity. Thus, the best way to evaluate PVC structure is to apply vibrational spectroscopy, the unique feature of which is high information capacity in the study of the different configurational and conformational states of macromolecules. Most of the Raman spectroscopic methods of PVC structure testing were developed more than 20 years ago. Therefore, improvement of the already recognized methods and development of modern computerbased processing of the spectra for the detailed quantitative analysis of PVC structure are important. In this work, we present an extensive study of polarized Raman spectra of a number of industrial grades of PVC powder and films, prepared from solutions of tetrahydrofuran and acetophenone. For PVC itself and residues of the solvents, remained in the films, we determined the number of Raman lines and their spectral characteristics. These data were used to assign accurately PVC Raman lines and to evaluate structure of different grades of PVC powder and films

Влияние ДНК-тропных антиканцерогенных соединений на механизмы регуляции экспрессии генов

The presented review is devoted to the analysis of molecular mechanisms of action for different natural DNA-tropic compounds with established tumor preventive activity. Here we present their cancer preventive effects observed in vivo, mechanisms of DNA binding, influence on epigenetic regulation and “housekeeping” protein function. Additionally, the influence of these compounds on DNA helix parameters is discussed that should impact on epigenetic regulation of gene expression and formation of topologically associated domains.Обзор посвящен анализу молекулярных механизмов действия ряда природных ДНК-тропных соединений с установленной антиканцерогенной активностью. Приведены данные исследований антиканцерогенного действия этих соединений в экспериментах in vivo, рассмотрены механизмы их связывания с ДНК, влияния на метилирование ДНК и модификацию гистонов, способность к ингибированию функций ферментов «домашнего хозяйства». Кроме того, проанализированы возможные эффекты этих соединений на характеристики дуплекса ДНК, что должно иметь значение для эпигенетической регуляции экспрессии генов и формирования топологически ассоциированных доменов

Influence of natural DNA-binding compounds with cancer preventive activity on chromatin structure and interferon signaling

Blokhin NMRCO, Department of Chemical Carcinogenesis, Moscow, Russia1 Roswell Park Cancer Institute, Department of Cell Stress Biology, Buffalo, USA2 Ryazan State Medical University, Ryazan, Russia3 RUDN University, Moscow, Russia4 Chromatin-destabilizing effects of the natural DNA-binding small molecules Curcumin, Quercetin, Resveratrol, Genistein, Fisetin, Coumarin, Apigenin, EGCG, Thymoquinon and Naringenin on chromatin structure were analyzed by histone H1 and chaperone of histone SSRPtranslocation in cell nuclear. Using live cell fluorescent imaging we demonstrated that all the natural DNA-binding compounds studied were able to cause the appearance of cell fractions with high histone H1 amount localized in nucleolus. Visible changes in location of SSRP in cells treated with all the phytonutrients was also observed by live cell fluorescent imaging and for the number of compounds its translocation from nucleoles was confirmed by Western Blotting. It should be noted that dynamics of the phytonutrients influence on H1 and SSRP translocation varied greatly. We revealed activation of INF signaling by Genistein, Curcumin, Kaempferol, Sanguinarin, Naringenin, Resveratrol, Fisetin and Quercetin using flow cytometry of the HeLa-TI cells with ISRE-mCherry reporter

Activation of interferon-α signaling by resveratrol, genistein and quercetin

Resveratrol, genistein and quercetin from the group of polyphenols from secondary plant metabolites reveal cancer preventive and antivirus effects realized via their pleiotropic influence on the different macromolecules in cells. These compounds can interact with DNA without the formation of covalent bonds. This process is usually followed by changes in spatial, physical-chemical and structural DNA characteristics that can result in disfunction of DNA metabolism enzymes and chromatin destabilization. Similar effects were described for anticancer drug Curaxine CBL0137 in association with activation of interferon-α signaling. We demonstrated dose-dependent stimulating effects of resveratrol, genistein and quercetin on interferon-α signaling using HeLa cells expressed mCherry protein with interferon-stimulated response elements (ISRE) in promoter. Furthermore, it was shown by live-cell fluorescent microscopy in HT1080 cells with mCherry-labeled histone H1.5 that described polyphenols induced the redistribution of this linker histone in cell nuclei. The data obtained suggest an existence of DNA-dependent mechanism of anticancer effects of plant polyphenols and a need for further study of crosslinks between the polyphenols’ influence on chromatin structure and the changes in genome function, in particular, induction of interferon-interferon-α signaling. © 2019, Tomsk National Research Medical Center of the Russian Academy of Sciences. All rights reserved