Expression of cancer-associated genes in prostate tumors

Background: Prostate cancer is one of the most common male cancers in Western countries and takes the third place in morbidity in Ukraine. It is a highly heterogeneous disease. Aim: To analyze relative expression levels of the TGFB1, IL1B, FOS, EFNA5, TAGLN, PLAU, and EPDR1 genes in malignant and non-malignant prostate tissues. Materials and Methods: Total RNA was isolated from 16 prostate adenomas, 37 prostate adenocarcinomas, and 29 conventionally normal prostate tissues. To analyze relative gene expression levels the quantitative real-time polymerase chain reaction was performed. Results: The significant alterations in the relative expression levels were found in all analyzed sample groups for 4 genes: FOS, EFNA5, IL1B, and TGFB1. We have found that FOS and EFNA5 were more frequently overexpressed in carcinomas with Gleason score ≤ 7, compared with adenomas. On contrary, PLAU expression levels were decreased more frequently in prostate cancers, compared with conventionally normal tissues. Noteworthy, we found positive correlation between IL1B expression level and PSA (for patients with slight PSA increase, no more than 20.0 ng/ml). Conclusion: The EFNA5, FOS, IL1B, PLAU, and TGFB1 genes that showed significant expression alterations in prostate tumors, compared with conventionally normal prostate tissue, may play role in prostate cancer development and should be further investigated

Non-Linear/Non-Commutative Non-Abelian Monopoles

Using recently proposed non-linearly realized supersymmetry in non-Abelian gauge theory corrected to the order (alpha')^2, we derive the non-linear BPS equations in the background B-field for the U(2) monopoles and instantons. We show that these non-Abelian non-linear BPS equations coincide with the non-commutative anti-self-dual equations via the Seiberg-Witten map.Comment: 9 pages, LaTe

E7(7) formulation of N=2 backgrounds

In this paper we reformulate N=2 supergravity backgrounds arising in type II string theory in terms of quantities transforming under the U-duality group E7(7). In particular we combine the Ramond--Ramond scalar degrees of freedom together with the O(6,6) pure spinors which govern the Neveu-Schwarz sector by considering an extended version of generalised geometry. We give E7(7)-invariant expressions for the Kahler and hyperkahler potentials describing the moduli space of vector and hypermultiplets, demonstrating that both correspond to standard E7(7) coset spaces. We also find E7(7) expressions for the Killing prepotentials defining the scalar potential, and discuss the equations governing N=1 vacua in this formalism.Comment: 40 pages, final version to appear in JHE

Differential Bio-Optoelectronic Gating of Semiconducting Carbon Nanotubes by Varying the Covalent Attachment Residue of a Green Fluorescent Protein

Publisher Copyright: © 2022 The Authors. Advanced Functional Materials published by Wiley-VCH GmbHIntegrating photoactive proteins with synthetic nanomaterials holds great promise in developing optoelectronic devices whereby light, captured by a antenna protein, is converted to a modulated electrical response. The protein–nanomaterial interface is critical to defining optoelectronic properties; successful integration of bionanohybrids requires control over protein attachment site and a detailed understanding of its impact on device performance. Here, the first single-walled carbon nanotube (SWCNT) bio-optoelectronic transistor enabled by the site-specific direct interfacing with a green fluorescent protein (GFP) via genetically encoded phenyl azide photochemistry is reported. The electrical behavior of individual semiconducting SWCNTs depends on the protein residue coupling site and provides the basis to design eco-friendly phototransistors and optoelectronic memory. Attachment at one GFP residue proximal to the chromophore produces a wavelength-specific phototransistor. The bio-transistor can be switchedoff in less than 38 s with responsivity up to 7 × 103 A W−1 at 470 nm. Attachment via a second residue distal to the chromophore generates optoelectronic memory that show rapid and reproducible conductivity switching with up to 15-fold modulation that is restored on the application of a gate voltage. Therefore, photoactive proteins, especially GFP, can be realized as a key material for novel single-molecule electronic and photonic devices.Peer reviewe

EXPRESSION OF CANCER-ASSOCIATED GENES IN PROSTATE TUMORS

Background: Prostate cancer is one of the most common male cancers in Western countries and takes the third place in morbidity in Ukraine. It is a highly heterogeneous disease. Aim: To analyze relative expression levels of the TGFB1, IL1B, FOS, EFNA5, TAGLN, PLAU, and EPDR1 genes in malignant and non-malignant prostate tissues. Materials and Methods: Total RNA was isolated from 16 prostate adenomas, 37 prostate adenocarcinomas, and 29 conventionally normal prostate tissues. To analyze relative gene expression levels the quantitative real-time polymerase chain reaction was performed. Results: The significant alterations in the relative expression levels were found in all analyzed sample groups for 4 genes: FOS, EFNA5, IL1B, and TGFB1. We have found that FOS and EFNA5 were more frequently overexpressed in carcinomas with Gleason score ≤ 7, compared with adenomas. On contrary, PLAU expression levels were decreased more frequently in prostate cancers, compared with conventionally normal tissues. Noteworthy, we found positive correlation between IL1B expression level and PSA (for patients with slight PSA increase, no more than 20.0 ng/ml). Conclusion: The EFNA5, FOS, IL1B, PLAU, and TGFB1 genes that showed significant expression alterations in prostate tumors, compared with conventionally normal prostate tissue, may play role in prostate cancer development and should be further investigated