Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis

A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis

Characterization of Actinomycetes Antagonist Fusariumoxysporum f.sp.passiflora Isolated from Rhizosphere Soil ofPurple Passion Fruit Plants, South Sulawesi, Indonesia

To survey rhizosphere actinomycetes as potential biocontrol against fungal diseaseof passion fruits, rhizosphere soil of the plant were used as an isolation sources. Twenty fivestrains were assigned to Streptomyces-like strain based on morphological properties of sporechain. Four strain with distinguishing characteristic based on the macroscopic appearance ofcolonies on different media, were recovery from rhizosphere soil of passion fruits plantsuggesting that various Streptomyces spp. grow surrounding of plant roots. On an agarmedium, four strains (11.43%) commonly formed a clear growth-inhibition zone against fungal pathogen of passion fruits, Fusarium oxysporum f.sp. radicalix passiflori (FORP), indicatingthat this strains can produce antifungal substances. The present results indicate that four strainare a suitable candidate for the biocontrol of fusarium wilt. Keywords: Actinomycetes, AntifungalFusarium oxysporum f.sp.passiflora Rhizosphere, Purple Passion Fruit Plants, South Sulawesi, Indonesi