134 research outputs found
Caractérisation moléculaire et pharmacologique des récepteurs cholinergiques de type nicotinique (nAChRs) chez les insectes (Thèse de Doctorat d'Université)
Characterization of nicotine acetylcholine receptor subunits in the cockroach Periplaneta americana mushroom bodies reveals a strong expression of β1 subunit: involvement in nicotine-induced currents
Nicotinic acetylcholine receptors are ligand-gated ion channels expressed in many insect structures, such as mushroom bodies, in which they play a central role. We have recently demonstrated using electrophysiological recordings that different native nicotinic receptors are expressed in cockroach mushroom bodies Kenyon cells. In the present study, we demonstrated that eight genes coding for cockroach nicotinic acetylcholine receptor subunits are expressed in the mushroom bodies. Quantitative real-time polymerase chain reaction (PCR) experiments demonstrated that β1 subunit was the most expressed in the mushroom bodies. Moreover, antisense oligonucleotides performed against β1 subunit revealed that inhibition of β1 expression strongly decreases nicotine-induced currents amplitudes. Moreover, co-application with 0.5 μM α-bungarotoxin completely inhibited nicotine currents whereas 10 μM d-tubocurarine had a partial effect demonstrating that β1-containing neuronal nicotinic acetylcholine receptor subtypes could be sensitive to the nicotinic acetylcholine receptor antagonist α-bungarotoxin
Caractérisation d’une cible d’insecticides chez le puceron du pois : Vers une lutte chimique ciblée contre les ravageurs
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