Визначення вмісту антоціанів і танінів у аронії чорноплідної плодах

Introduction. Aronia melanocarpa is widely cultivated in Ukraine as a food, medicinal and ornamental plant. The quality of fresh fruit of Aronia melanocarpa was regulated by the requirements of the pharmaceutical article 42-66-87 "Fresh fruit of Aronia melanocarpa", but the article required a revision using modern approaches to the standardization of medicinal plant material.The aim of the study – determination of the content of anthocyanins and tannins in fresh and dried fruit of Aronia melanocarpa for inclusion the results of the research in the section of the monograph "Quantitative determination".Research Methods. The determination of the content of anthocyanins and tannins in fresh and dried fruit of Aronia melanocarpa was carried out by the method of absorption spectrophotometry.Results and Discussion. The content of anthocyanins in fresh fruit of Aronia melanocarpa is from (0.45±0.01) % to (0.56±0.02) % in terms of cyanidin-3-O-glucoside chloride, the content of tannins in dried fruit of Aronia melanocarpa is from (1.51±0.02) % to (2.41±0.03) % in terms of pyrogallol and dry raw materials. Therefore we proposed to enter the indicator: the content of anthocyanins – at least 0.40 % in terms of cyanidin-3-O-glucoside chloride to the national monograph "Fresh fruit of Aronia melanocarpaN", and the indicator: the content of tannins – not less than 1.5 % in terms of pyrogallol and dry raw materials to the national monograph "Dried fruit of Aronia melanocarpaN".Conclusion. By the method of absorption spectrophotometry in fresh fruit of Aronia melanocarpa the content of anthocyanins was determined, in dried fruit of Aronia melanocarpa – tannins. The results of the research were used during the development of the national monographs "Fresh fruit of Aronia melanocarpaN" and "Dried fruit of Aronia melanocarpaN".Вступление. Арония черноплодная (Aronia melanocarpa) широко культивируется в Украине как пищевое, лекарственное и декоративное растение. Качество плодов аронии черноплодной свежих регламентировалось требованиями ФС 42-66-87 “Плоды аронии черноплодной (рябины черноплодной) свежие”, однако статью необходимо было пересмотреть с использованием современных подходов к стандартизации лекарственного растительного сырья.Цель исследования – определить содержание антоцианов и танинов в плодах аронии черноплодной свежих и высушенных для включения результатов исследований в раздел монографии “Количественное определение”.Методы исследования. Содержание антоцианов и танинов в аронии черноплодной плодах свежих и высушенных определяли методом абсорбционной спектрофотометрии.Результаты и обсуждение. Содержание антоцианов в аронии черноплодной плодах свежих составляет от (0,45±0,01) до (0,56±0,02) % в пересчете на цианидин-3-O-глюкозида хлорид, содержание таниновв аронии черноплодной плодах высушенных – от (1,51±0,02) до (2,41±0,03) % в пересчете на пирогаллол и сухое сырье. Поэтому в национальную монографию “Аронии (рябины) черноплодной плоды свежиеN”предложено ввести показатель: содержание антоцианов – не менее 0,40 % в пересчете на цианидин-3-O-глюкозида хлорид, а в национальную монографию “Аронии (рябины) черноплодной плоды высушенныеN” – показатель: содержание танинов – не менее 1,5 % в пересчете на пирогаллол и сухое сырье.Вывод. Методом абсорбционной спектрофотометрии в плодах аронии черноплодной свежих определено содержание антоцианов, в плодах аронии черноплодной высушенных – танинов. Результаты исследований использовано при разработке национальных монографий “Аронии (рябины) черноплодной плоды свежиеN” и “Аронии (рябины) черноплодной плоды высушенныеN”.Вступ. Аронія чорноплідна (Aronia melanocarpa) широко культивується в Україні як харчова, лікарська і декоративна рослина. Якість аронії чорноплідної плодів свіжих регламентувалася вимогами ФС 42-66-87 “Плоди аронії чорноплідної (горобини чорноплідної) свіжі”, проте статтю треба було переглянути з використанням сучасних підходів до стандартизації лікарської рослинної сировини.Мета дослідження – визначити вміст антоціанів і танінів у аронії чорноплідної плодах свіжих та висушених для включення результатів досліджень до розділу монографії “Кількісне визначення”.Методи дослідження. Вміст антоціанів і танінів в аронії чорноплідної плодах свіжих та висушених визначали методом абсорбційної спектрофотометрії.Результати й обговорення. Вміст антоціанів у аронії чорноплідної плодах свіжих становить від (0,45±0,01) до (0,56±0,02) % у перерахунку на ціанідин-3-O-глюкозиду хлорид, вміст танінів у аронії чорноплідної плодах висушених – від (1,51±0,02) до (2,41±0,03) % у перерахунку на пірогалол і суху сировину. Тому до національної монографії “Аронії (горобини) чорноплідної плоди свіжіN” запропоновано ввести показник: вміст антоціанів – не менше 0,40 % у перерахунку на ціанідин-3-O-глюкозиду хлорид, а до національної монографії “Аронії (горобини) чорноплідної плоди висушеніN” – показник: вміст танінів – не менше 1,5 % у перерахунку на пірогалол і суху сировину.Висновок. Методом абсорбційної спектрофотометрії в аронії чорноплідної плодах свіжих визначено вміст антоціанів, у аронії чорноплідної плодах висушених – танінів. Результати досліджень використано при розробці національних монографій “Аронії (горобини) чорноплідної плоди свіжіN” й “Аронії (горобини) чорноплідної плоди висушеніN”

Tunable coaxial cavity resonator for linear and nonlinear microwave characterization of superconducting wires

We discuss experimental results obtained using a tunable cylindrical coaxial cavity constituted by an outer Cu cylinder and an inner Pb-BSCCO wire. We have used this device for investigating the microwave response of the superconducting wire, both in the linear and nonlinear regimes. In particular, by tuning the different modes of the cavity to make them resonant at exactly harmonic frequencies, we have detected the power emitted by the superconducting inner wire at the second- and third-harmonic frequency of the driving field. The results obtained in the nonlinear regime, whether for the microwave surface impedance or the harmonic emission, are qualitatively accounted for considering intergrain fluxon dynamics. The use of this kind of device can be of strong interest to investigate and characterise wires of large dimensions to be used for implementing superconducting-based microwave devices.Comment: 14 pages, 6 embedded figures, accepted for publication in Supercond. Sci. Techno

Using multilayer polymer PI/Pb composites for protection against X-ray bremsstrahlung in outer space

This paper presents data about the radiation-protective characteristics of multilayer polyimide/lead (PI/Pb) composites for X-ray radiation. Multilayer composites were obtained by collecting lead-filled polyimide track membranes into a sandwich of the required thicknes

Verification of Mathematical Models of Plague

Mathematic modeling and prognostication of infectious diseases epidemic process is a promising trend of epidemiologic investigations. The complex of mathematic models (SEIRF type) of plague epidemic process was developed for this purpose by the Russian Research Anti-Plague Institute “Microbe” and laboratory of epidemiologic cybernetics of N.F.Gamaleya Institute for Epidemiology and Microbiology. The data on the plague outbreak in 1945 in the rural settlement Avan’ (Aral region of Kzyl-Orda district of Kazakh SSR) were used to test working efficiency of this complex. The data analysis permitted to obtain the starting and boundary conditions for epidemic process modeling. In the process of modeling the mathematical models of epidemic process of plague with various ways of infection transmission for each epidemic focus in regard with historical data were used. The data were processed by the analytical platform Deductor 5.1. Identified was strong positive correlation between estimated and historical data – r = +0,71. The results received testify that mathematic models are effective and give high degree of confidence. They can be used to receive quantitative characteristics of prognosis for plague epidemic process development with different transmission routes considering that anti-epidemic measures have been taken

Characteristic of water of the Lena River and health of the population of the Republic of Sakha (Yakutia)

In the article results of sanitary epidemiological characteristics of water of the Lena River, including on indicators of parasitic safety and physiological full value are analysed. It is established that the adverse effect of water of a water source on health of the population is connected with its physiological inferiority which is caused by the low maintenance of a number of biologically active elements. The received value of factor of "utility of water» in 47 times below recommended value

Factors of local conversion of iodothyronines correlate with indicators of hormonal, biochemical, and hematological profiles in patients with spinal canal and dural sac stenosis of the lumbar spine

In order to find out the mechanisms of pathogenesis of degenerative-dystrophic diseases of the spine, it is of particular interest to search for body parameters which are directly or indirectly interrelated with the key factors of peripheral conversion of nidus iodothyronines and constitute a system of network interactions, affecting metabolic indicators at the local and systemic level.The aim. To search for correlations of local key factors of peripheral conversion of Ligamentum flavum iodothyronines with indicators of biochemical, hematological and hormonal blood profiles of patients with stenosing processes of the spinal canal and dural sac in the lumbar spine.Materials and methods. 33 patients (15 males, 18 females) with stenosing processes of the spinal canal and dural sac in the lumbar spine were examined (mean age – 45.73 ± 1.95 years). The expression of deiodinase genes and other candidate genes was determined in Ligamentum flavum biopsies collected during surgical treatment. Biochemical, hematological and hormonal parameters were determined in peripheral blood. The resulting data array was processed in order to find correlations between the parameters of systemic and local metabolism.Results. The relationships of deiodinases with the expression of GDF5, MMP1, MMP3 and TIMP1 in Ligamentum flavum (p < 0.05) were found. Of the hormonal profile of the blood serum, the most significant indicators were thyreotropin, free triiodothyronine and thyroperoxidase antibodies. In the biochemical profile, levels of direct bilirubin, total cholesterol, HDL and LDL cholesterol and triglycerides changed along with the expression of deiodinases. Correlative relationships with the expression of deiodinases were found for the following hematological analytes of whole peripheral blood: hemoglobin, mean corpuscular hemoglobin concentration, numbers of granulocytes, lymphocytes, eosinophils, monocytes, band neutrophils, red cell distribution width and platelet crit. The data obtained indicate the involvement of peripheral conversion factors in the pathogenetic process and provide information to form a new view on the pathogenesis of degenerative-dystrophic processes in the Ligamentum flavum of patients with stenosing processes of the spinal canal and the dural sac in the lumbar spine

Создание новых форм томата с генами устойчивости к грибным болезням на основе маркерной селекции

Relevance. The presented studies are aimed at obtaining new forms of tomato with a complex of genes for resistance to fungal diseases in combination with a standard type of bush and dark coloring of fruits based on marker-mediated selection.Methodology. The biological objects of the study are varieties and hybrid forms of tomato from the collection of the Michurinsky SAU. Molecular genetic analysis was performed using the following methods. DNA extraction was carried out from young leaves using a kit for isolation of NC Sample NC manufactured by Agrodiagnostika LLC according to the manufacturer's protocol. Fermentas production kits were used for PCR. Identification of the cladosporosis resistance gene was Cf-19 performed using the DNA marker R7. The presence of a fusarious wilting resistance gene was determined by a I-2/5 marker. The amplification results were visualized by agarose gel electrophoresis.Results. During the research, a collection of varieties and hybrid forms of tomato of the Michurinsky GAU was analyzed in order to identify genes for resistance to cladosporiosis Cf-19 and fusarium wilt I-2. A total of 52 genotypes were analyzed. It was found that most samples (41 samples) are characterized by a heterozygous state of the Cf-19 gene. All indeterminant and semi-determinant forms had both alleles. Of the 23 determinant forms presented in the collection, 10 had only one allele corresponding to recessive homozygote. Among all analyzed tomato genotypes, no dominant homozygous forms were noted. The study of the collection revealed several alleles of the I-2 gene. In total, four fragments corresponding to various alleles were amplified. A total of 50 resistant genotypes have been identified in the collection. Two alleys of the I-2 gene (633/693 bp) were identified in 42 tomato samples. Four varieties are homozygous in one allele (633 bp), which determines resistance. Three varieties have a second resistance allele (566 bp). One genotype has only an allele defining susceptibility (693 bp). On the basis of molecular analysis, as well as an assessment of the type of bush and fetal color, initial forms were selected with subsequent hybridization. 67 hybrid tomato plants were obtained. Evaluation of the presence of resistance genes showed that most of the resulting hybrids are resistant to cladosporiosis and fuzariosis. This is due to the presence of dominant alleles of Cf-19 and I-2 genes in a heterozygous state. Among the resulting hybrids, plants with a bark type of bush were identified. A total of 13 such plants were obtained.Conclusion. Thus, the work carried out allowed to obtain hybrid forms of tomato combine the signs of resistance to two pathogens of fungal diseases and the stem type of the bush. These forms are planned to be used in further selection work.Цель. Исследования направлены на получение новых форм томата с комплексом генов устойчивости к грибным болезням в сочетании со штамбовым типом куста и темной окраской плодов на основе маркер-опосредованной селекции.Методы. Объект исследований – сорта и гибридные формы томата из коллекции Мичуринского ГАУ. Молекулярно-генетический анализ проводили с использованием следующих методов. Экстрагирование ДНК осуществляли из молодых листьев с применением набора для выделения НК «Проба НК» производства ООО «Агродиагностика» согласно протоколу производителя. Для проведения ПЦР использованы наборы производства компании Fermentas. Идентификацию гена устойчивости к кладоспоризу Cf-19 проводили с использованием ДНК-маркера Р7. Наличие гена устойчивости к фузариозному увяданию определяли с помощью маркера I-2/5. Визуализацию результатов амплификации осуществляли с помощью электрофореза в агарозном геле.Результаты. При проведении исследований была проанализирована коллекция сортов и гибридных форм томата Мичуринского ГАУ с целью идентификации генов устойчивости к кладоспориозу Cf-19 и фузариозному увяданию I-2. Всего проанализировано 52 генотипа. Установлено, что для большинства образцов (41 образец) характерно гетерозиготное состояние гена Cf-19. Все индетерминантные и полудетерминантные формы имели оба аллеля. Из 23 представленных в коллекции детерминантных форм у 10 отмечен только один аллель, соответствующий рецессивной гомозиготе. Среди всех анализируемых генотипов томата не отмечено доминантных гомозиготных форм. Изучение коллекции позволило выявить нескольких аллелей гена I-2. Всего амплифицировано четыре фрагмента, соответствующих различным аллелям. Всего устойчивых генотипов в коллекции выделено 50. У 42 образцов томата идентифицированы два аллея гена I-2 (633/693 п.н). Четыре сорта гомозиготны по одному аллелю (633 п.н.), обуславливающему устойчивость. Три сорта имеют второй аллель (566 п.н.) устойчивости. Один генотип имеет только аллель определяющий восприимчивость (693 п.н.).На основании молекулярного анализа, а также оценки типа куста и окраски плода был проведен отбор исходных форм с последующей гибридизацией. Получено 67 гибридных растений томата. Оценка наличия генов устойчивости показала, что большинство полученных гибридов являются устойчивыми к кладоспориозу и фузариозу. Это обусловлено наличием доминантных аллелей генов Cf-19 и I-2 в гетерозиготном состоянии. Среди полученных гибридов выделены растения со штамбовым типом куста. Всего таких растений получено 13. Проведенная работа позволила получить гибридные формы томата, сочетающие признаки устойчивости к двум возбудителям грибных болезней и штамбовый тип куста. Эти формы планируется использовать в дальнейшей селекционной работе

Features of type 2 diabetes mellitus and its pharmacotherapy in outpatients

Background. Type 2 diabetes mellitus (DM) remains the most common type of DM and is associat-ed with disabling complications, reduced quality of life and reduced life expectancy. Satisfactory control of carbohydrate metabolism remains the key way to manage them. Aim. To perform a retrospective analysis of carbohydrate metabolism (in terms of glycated hemoglobin – HbA1c), the prevalence of complications, and features of hypoglycemic and concomitant therapy in patients with type 2 DM. Materials and methods. The analysis of sex and age characteristics, achieved level of HbA1c, diabetes complications, sugar-reducing and concomitant therapy according to the data of outpatient records of the patients who are on dispensary registration with an endocrinologist in the Endocrinology Department of the Consultative and Diagnostic Polyclinic of the Tomsk Regional Clinical Hospital in Tomsk was carried out. Results. 546 outpatient medical records of patients with type 2 DM were analysed, among which there were 39.6% men (n=216) with a history of type 2 DM 8.0 years [3.0; 13.0] , median age 64.0 years [54.5; 71.0] and 60.4% women (n=330), history of type 2 DM 10.0 years [5.0; 15.0], median age 70.0 years [63.0; 75.0]. The achieved HbA1c level in men was 7.6% [6.3; 9.0] and in women 7.4% [6.4; 9.1]. 19.4% of men and 13.6% of women had an aggravated history of type 2 DM. According to the history, 6.5% of men (n=14) and 3% of women (n=10) with type 2 DM had a history of stroke, and myocardial infarction 12% (n=26) and 1.5% (n=5), respectively. Among the analysed outpatient records of type 2 DM patients, 18.5% of men (n=40) and 12.4% of women (n=41) were found to have diabetic nephropathy. Diabetic retinopathy was reported in 9.3% (n=20) of men and 4.2% (n=14) of women. Diabetic macroangiopathies were detected in 29.6% (n=64) of males and 9.7% (n=32) of females. Among other chronic complications of DM, diabetic neuroosteoarthropathy was recorded in 1% (n=2) of males and 3% (n=10) of females, diabetic polyneuropathy in 25% (n=54) and 21.5% (n=71), respectively. Diabetic foot was diagnosed in 1.9% (n=4) of men and 1.8% (n=6) of women. Among comorbid pathology, obesity was diagnosed in 45.4% (n=88) of men and 69.1% (n=228) of women, dyslipidaemia in 10.2% (n=22) and 10.6% (n=35) respectively, hypertension in 39.8% (n=86) and 32.6% (n=108) of cases. The diagnosis of non-alcoholic fatty liver disease was verified in 3.7% of men (n=7) and 1.8% of women (n=6), chronic heart failure in 7.4% of men (n=16) and 2.4% of women (n=8) registered for type 2 DM. According to the analysed outpatient records, 4.1% (n=23) of patients received diet therapy, 48.3% (n=263) received monotherapy and 47.6% (n=260) received combination therapy for type 2 DM. Metformin was the most commonly used monotherapy for type 2 DM 36.1% (n=197), followed by insulin 6.9% (n=38), sulfonylurea derivatives – 2.7% (n=15). Combination of metformin and dipeptidyl peptidase-4 inhibitors (13.9%) was the most commonly used combination therapy. Conclusion. Analysis of the current situation in the diabetology service will help to identify weaknesses and strengths, which is necessary to optimise existing therapeutic approaches in accordance with current clinical recommendations

ELEVATE-TN Study. New data of acalabrutinib in first-line treatment of chronic lymphocytic leukemia. Resolution

Over the past decade, we have seen a significant change in modern approaches in the first-line treatment of chronic lymphocytic leukemia (CLL). The CLL-10 study data established the FCR regimen as the treatment of choice for younger patients with limited comorbidities, while for patients older than 65 years, the BR regimen is more often considered as less toxic one. According to published data, 46% of patients with newly diagnosed CLL have comorbidities. Moreover, high-risk patients with del(17p) and/or TP53 mutation do not have response on immunochemotherapy (ICT) most often. Thus, about 1/2 of the patients cannot be treated or will not respond to standard ICT regimens. Targeted therapy with Brutons tyrosine kinase (BTK) inhibitors is an important option of the first-line treatment of patients with CLL. Acalabrutinib is a highly selective second-generation BTK inhibitor that does not inhibit EGFR, ITK or TEC targets. Acalabrutinib in combination with obinutuzumab or as monotherapy can be considered as a highly effective and safe option of the first line of CLL therapy. Based on the hight selectivity of the agent, acalabrutinib can be considered as the preferable option for patients who are not eligible for ICT, including patients with commodities, such as cardiovascular diseases or risk factors for their development

Features of developing SARS-CoV-2 nucleocapsid protein population-based seroprevalence during the first wave of the COVID-19 epidemic in the Russian Federation

The novel coronavirus (SARS-CoV-2) pandemic, dubbed COVID-19, has become one of the most serious challenges for human populations in the vast majority of countries worldwide. Rapid spreading and increased mortality related to it required new approaches to manage epidemic processes on a global scale. One of such approaches was based on analyzing SARS-CoV-2 seroprevalence associated with COVID-19. Our aim was to summarize the results on assessing seroprevalence to the SARS-CoV-2 nucleocapsid antigen (Nc) in residents from 26 regions of the Russian Federation, carried out during the first wave of the COVID-19 epidemic.Materials and methods. Seroprevalence distribution was examined in 26 model regions of the Russian Federation according to the unified method developed by the Rospotrebnadzor with the participation of the Federal State Institution Saint Petersburg Pasteur Research Institute of Epidemiology and Microbiology. Such approach implied formation of a group of volunteer subjects in model geographic region who were tested by ELISA for anti-Nc serum antibody level in peripheral blood. Analyzed primary data obtained in separate regions were either accepted for publication or released.Results. The current paper finalizes the data obtained in all 26 regions of the Russian Federation. The total SARS-CoV-2 seroprevalence was 19.5 (10.0–25.6)% with the maximum and minimum value found in the Kaliningrad Region and the Republic of Crimea, respectively (50.2% vs. 4.3%). A pattern of age-related seroprevalence distribution indicates insignificant predominance of seroprevalence among subjects of 1–17 years old: 22.1 (13.1–31.8)%. Among COVID-19 convalescents positive for SARS-CoV Nc antibodies it reached 60.0 (40.0–73.3)%. The number of contact persons comprised 6285 subjects or 8.5% of total volunteer cohort, with the level of seroprevalence reaching up to 25.3 (17.95–35.8)%. A direct correlation was revealed between levels of seroprevalence in convalescent and contact volunteers. In addition, the reproductive number for SARS-CoV was calculated comprising 5.8 (4.3–8.5) suggesting that one convalescent subject can infect at least 4 healthy individuals. A high level of asymptomatic forms of COVID-19 among seropositive subjects was confirmed empirically comprising up to 93.6 (87.1–94.9)%.Conclusion. A single cross-sectional study performed during 2020 June–August timeframe allowed to assess pattern of sex- and agerelated COVID-19 seroprevalence for general population in 26 Russian Federation regions. The data obtained may serve as a basis for the longitudinal cohort investigation with serial subject sampling. The timing and duration of study will be determined by dynamics of ongoing COVID-19 epidemic