Dynamics of risk degree of innovation-driven growth at higher education institutions

In this article, the analysis of dynamics of risk degree of innovation-driven growth at higher education institutions is discussed. The survey has been employed in this paper and the data analyzed by SPSS software. Results revealed that the resulting risks are regularly encountered in a modern Russian higher educational institution: inability to provide necessary financial support for educational activities; inability to provide required modern material and technical basis; challenges and failures in collaboration with enterprises for organizing practical training for students and the absence of practical training programs at the university. It is also indicated that the risk event might lead to different results: negative, positive, or nul

Клинико-иммунологическая эффективность интерферонотерапии при ротавирусной инфекции у детей раннего возраста

Purpose: to improve the complex therapy of rotavirus infection in infants by including interferon and to evaluate its clinical and immunological effectiveness.Materials and methods. Infants without infectious pathology and with rotavirus infection, who were on standard therapy and treatment, including standard therapy and interferon (20 in each group), were examined using general clinical methods. The diagnosis of rotavirus infection was carried out by molecular-genetic method and immunochromatography. Indicators of cellular immunity were determined by flow cytometry. Statistical processing of the results was carried out using the STATISTICA 8.0 program for related and independent samples using the nonparametric Mann-Whitney test and χ2.Summary. In all infants with rotavirus infection, the disease occurred in a moderate form with symptoms of grade II exicosis. In the group of patients who received an additional interferon drug as part of complex therapy, the duration of intoxication symptoms was significantly less, and in the hemogram at discharge from the hospital, the relative content of neutrophils was higher and monocytes lower compared to infants who were only on standard treatment, which corresponded to the norm. In the acute period of rotavirus infection, the relative level of lymphocytes decreased, and NK cells and T-NK cells increased compared to healthy infants. The relative number of cells expressing TLR-3 at the beginning of the disease was lower than in the control group. The inclusion of interferon in the complex therapy contributes to normalization of the relative number of NK cells, while maintaining an increased content of T-NK cells in both relative and absolute terms compared to healthy infants, and a reduced content of cells expressing TLR-3.Conclusion. Currently, rotavirus infection in infants is typical, the most pronounced and long-lasting clinical symptom of the disease is watery diarrhea. The use of interferon in complex therapy helps to reduce the duration of intoxication and normalize the hemogram. Changes in the immune status in the acute period of rotavirus infection indicate increased antigenic stimulation of the cell link and inhibition of the synthesis of pro-inflammatory cytokines, including interferons, under the influence of rotavirus, which is the justification for the use of interferon therapy.Цель: совершенствование комплексной терапии ротавирусной инфекции у детей раннего возраста посредством включения препарата интерферона и оценка его клинико-иммунологической эффективности.Материалы и методы. Дети без инфекционной патологии и с ротавирусной инфекцией, находившиеся на стандартной терапии и лечении, включающем стандартную терапию и препарат интерферона (по 20 в каждой группе), обследованы с применением общеклинических методов. Диагностика ротавирусной инфекции проводилась молекулярно-генетическим методом и иммунохроматографически. Показатели клеточного иммунитета определялись методом проточной цитометрии. Статистическая обработка результатов осуществлялась с помощью программы STATISTICA 8.0 для связанных и независимых выборок с использованием непараметрического критерия Манна — Уитни и χ2.Результаты. У всех детей с ротавирусной инфекцией заболевание протекало в среднетяжелой форме с симптомами эксикоза II степени. В группе пациентов, получавших в составе комплексной терапии дополнительно препарат интерферона, продолжительность симптомов интоксикации наблюдалась достоверно меньше, а в гемограмме при выписке из стационара относительное содержание нейтрофилов было выше и моноцитов ниже по сравнению с детьми, находившимися лишь на стандартном лечении, что соответствовало норме. В острый период ротавирусной инфекции относительный уровень лимфоцитов снижался, а NK-клеток и Т-NK-клеток повышался по сравнению со здоровыми детьми. Относительное количество клеток, экспрессирующих TLR-3, в начале заболевания было ниже, чем в группе контроля. Включение в комплексную терапию препарата интерферона способствует нормализации относительного количества NK-клеток при сохранении повышенного содержания Т-NK-клеток как в относительном, так и в абсолютном выражении по сравнению со здоровыми детьми, и пониженного содержания клеток, экспрессирующих TLR-3.Заключение. В настоящее время ротавирусная инфекция у детей раннего возраста протекает типично, наиболее выраженным и длительно сохраняющимся клиническим симптомом заболевания является водянистая диарея. Применение в комплексной терапии препарата интерферона способствует сокращению длительности интоксикации и нормализации гемограммы. Изменения иммунного статуса в острый период ротавирусной инфекции свидетельствуют о повышенной антигенной стимуляции клеточного звена и угнетении под влиянием ротавируса синтеза провоспалительных цитокинов, в том числе интерферонов, что является обоснованием применения интерферонотерапии

Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C

The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein C was synthesized and the murine immune scFv library against this peptide was constructed. The protein C specific scFv 9E were selected from the constructed library by the phage-display method. The scFv 9E dissociation constant was found to be 2∙10-9 М. It was shown that scFv 9E were suitable for protein C detection by ELISA and Western blotting. Selected scFv could be further used for protein C investigation and for the development of quantitative methods for protein C detection in human blood

DYNAMICS OF CD34/CD45 POSITIVE CELLS CONCENTRATION IN PERIPHERAL BLOOD OF PATIENTS AFTER ORTHOTOPIC LIVER TRANSPLANTATION AND PARTIAL LIVER RESECTION

Number of CD34/CD45+ cells were determined in blood of pediatric recipients and adult living-related liver do- nors. It has been investigated 15 pairs of recipients (aged from 4 to 60 months) and donors (29 ± 5 years). All the children had liver cirrhosis caused by congenital and inherited diseases of hepatobiliary system. Concentration of CD34/CD45+ cells in venous blood of recipients was higher than in donors. In recipients level of CD34/CD45+ cells did not correlate with sex, age, weight and height but did correlate with some biochemical parameters related to liver function. After liver transplantation number of CD34/CD45+ cells did not change in peripheral blood of recipients. In donors partial liver resection did not cause statistically significant changes in number or concentration of CD34/CD45+ cells in blood. Presented data suggests that neither liver transplantation nor liver resection has remarkable influence on number of CD34/CD45 + cells in peripheral blood