Biosynthetic features and properties of xylose isomerases from Arthrobacter nicotianae, Escherichia coli, and Erwinia carotovora subsp

Abstract -The characteristics of xylose isomerase biosynthesis in the bacteria Arthrobacter nicotianae BIM B-5, Erwinia carotovora subsp atroseptica jn42xylA , and Escherichia coli HB101 xylA have been studied. The bacteria produced the enzyme constitutively. Out of the carbon sources studied, D -glucose and D -xylose were most favorable for the biosynthesis of xylose isomerase in E. carotovora subsp. atroseptica , but the least appropriate in terms of the enzyme production efficiency in E. coli . Minimum and maximum levels of xylose isomerase formation in A. nicotianae were noted, respectively, during D -xylose and sucrose utilization. An addition to the D -xylose-containing nutrient medium of 0.1-1.5% D -glucose did not affect the enzyme synthesis in A. nicotianae , but suppressed it in Erwinia carotovora subsp. atroseptica (by 7% at the highest concentration) and Escherichia coli (by 63 and 75% at concentrations of 0.1 and 1.0%, respectively). The enzyme proteins produced by the bacteria exhibited the same substrate specificity and electrophoretic mobility (PAGE) as xylose isomerase A. nicotianae , although insignificant differences in the major physicochemical properties were noted

Regeneration of Skin Surface by Multipotent Mesenchymal Stem Cells of Adipose Tissue in Laboratory Animals with Infected Wounds

This paper presents results of experimental studies in laboratory animals with a simulated infected wound, for which mesenchymal stem cells (MSCs) derived from adipose tissue were used in its treatment. The following peculiarities of MSCs for regeneration of skin defects are established: faster arrest of inflammation, accelerated wound healing processes, as well as observed stimulation of growth of skin appendages. The results of this study may serve the basis for further research from development to introduction into clinical practice of cellular technologies for the treatment of infected wound of various etiologies. [Med-Science 2013; 2(2.000): 601-17

Medicine Science 2013;2(2):601-17 Mesenchymal Stem Cells and Skin Regeneration Original Investigation Regeneration of Skin Surface by Multipotent Mesenchymal Stem Cells of Adipose Tissue in Laboratory Animals with Infected Wounds Medicine Science 2013;2(2

Abstract This paper presents results of experimental studies in laboratory animal