Turbulent Kinetic Energy Dissipation in Barrow Canyon

Pacific Water flows across the shallow Chukchi Sea before reaching the Arctic Ocean, where it is a source of heat, freshwater, nutrients, and carbon. A substantial portion of Pacific Water is routed through Barrow Canyon, located in the northeast corner of the Chukchi. Barrow Canyon is a region of complex geometry and forcing where a variety of water masses have been observed to coexist. These factors contribute to a dynamic physical environment, with the potential for significant water mass transformation. The measurements of turbulent kinetic energy dissipation presented here indicate diapycnal mixing is important in the upper canyon. Elevated dissipation rates were observed near the pycnocline, effectively mixing winter and summer water masses, as well as within the bottom boundary layer. The slopes of shear/stratification layers, combined with analysis of rotary spectra, suggest that near-inertial wave activity may be important in modulating dissipation near the bottom. Because the canyon is known to be a hotspot of productivity with an active benthic community, mixing may be an important factor in maintenance of the biological environment.Keywords: Continental shelf/slope, Arctic, Small scale processes, Mixin

The Effect of Race/Ethnicity on the Age of Colon Cancer Diagnosis

ABSTRACT BACKGROUND: Colorectal cancer is the third most commonly diagnosed cancer in the United States. Notably, racial/ethnic disparities exist in both incidence and mortality. PURPOSE: The aim of this case study was to investigate the impact of race/ethnicity on age at diagnosis of colorectal cancer in a defined population in Suffolk County, NY. METHODS: Data were retrospectively collected on race/ethnicity, health insurance status, age at diagnosis, stage at diagnosis, gender, smoking status, alcohol intake, tumor location, and body mass index for colorectal cancer patients with medical records in the Stony Brook University Medical Center database (2005-2011). Population-based data on Hispanic and non-Hispanic Whites were obtained from the Surveillance, Epidemiology, and End Results registry of New York State for an overlapping time period. Permutation-based ANCOVA and logistic regression with stepwise variable selection were conducted to identify covariates and first-order interactions associated with younger age at diagnosis and cancer stage as a dependent categorical variable. RESULTS: Of 328 colorectal cancer patients, Hispanics were diagnosed at a median younger age of 57y vs. 67y than non-Hispanic Whites (FDR = 0.001). Twenty-six percent of Hispanics were diagnosed with colorectal cancer prior to the recommended age (50y) for colorectal cancer surveillance compared to 11% of non-Hispanic Whites (FDR =0.007). Analysis of New York State registry data corroborated our findings that Hispanic colorectal cancer patients were diagnosed at a median younger age than non-Hispanic Whites. Permutation-based ANCOVA identified race/ethnicity and health insurance as significantly associated with age of diagnosis (P=0.001). Logistic regression selected (younger) age at diagnosis as being significantly associated with stage IV disease. The limitations of the case study reside in the use of self-reporting of race and ethnicity and in the small sample sizes. CONCLUSIONS: Hispanics may be at higher risk for colorectal cancer (y) and younger age at diagnosis is associated with advanced disease

Nonlinear internal waves over New Jersey's continental shelf

Author Posting. © American Geophysical Union, 2011. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 116 (2011): C03022, doi:10.1029/2010JC006332.Ship and mooring data collected off the coast of New Jersey are used to describe the nonlinear internal wave (NLIW) field and the background oceanographic conditions that formed the waveguide on the shelf. The subinertial, inertial, and tidal circulation are described in detail, and the background fluid state is characterized using the coefficients of the extended Korteweg–de Vries equation. The utility of this type of analysis is demonstrated in description of an amplitude-limited, flat wave. NLIWs observed over most of the month had typical displacements of −8 m, but waves observed from 17–21 August were almost twice as large with displacements near −15 m. During most of the month, wave packets occurred irregularly at a fixed location, and often more than one packet was observed per M2 tidal period. In contrast, the arrival times of the large-amplitude wave groups observed over 17–21 August were more closely phased with the barotropic tide. The time span in which the largest NLIWs were observed corresponded to neap barotropic conditions, but when the shoreward baroclinic energy flux was elevated. During the time of large NLIWs, near-inertial waves were a dominate contributor to the internal motions on the shelf and apparently regulated wave formation, as destructive/constructive modulation of the M2 internal tide by the inertial wavefield at the shelf break corresponded to stronger/weaker NLIWs on the shelf.This work was funded by the Office of Naval Research

Observations and modeling of a hydrothermal plume in Yellowstone Lake

Author Posting. © American Geophysical Union, 20XX. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 46(12), (2019): 6435-6442, doi:10.1029/2019GL082523.Acoustic Doppler current profiler and conductivity‐temperature‐depth data acquired in Yellowstone Lake reveal the presence of a buoyant plume above the “Deep Hole” hydrothermal system, located southeast of Stevenson Island. Distributed venting in the ~200 × 200‐m hydrothermal field creates a plume with vertical velocities of ~10 cm/s in the mid‐water column. Salinity profiles indicate that during the period of strong summer stratification the plume rises to a neutral buoyancy horizon at ~45‐m depth, corresponding to a ~70‐m rise height, where it generates an anomaly of ~5% (−0.0014 psu) relative to background lake water. We simulate the plume with a numerical model and find that a heat flux of 28 MW reproduces the salinity and vertical velocity observations, corresponding to a mass flux of 1.4 × 103 kg/s. When observational uncertainties are considered, the heat flux could range between 20 to 50 MW.The authors thank Yellowstone National Park Fisheries and Aquatic Sciences, The Global Foundation for Ocean Exploration, and Paul Fucile for logistical support. This research was supported by the National Science Foundation grants EAR‐1516361 to R. S., EAR‐1514865 to K. L., and EAR‐1515283 to R. H. and J. F. All work in Yellowstone National Park was completed under an authorized Yellowstone research permit (YELL‐2018‐SCI‐7018). CTD and ADCP profiles reported in this paper are available through the Marine Geoscience Data System (doi:10.1594/IEDA/324713 and doi:10.1594/IEDA/324712, accessed last on 17 April 2019, respectively).2019-11-0

Mode 2 waves on the continental shelf : ephemeral components of the nonlinear internal wavefield

Shoreward propagating, mode 2 nonlinear waves appear sporadically in mooring records obtained off the coast of New Jersey in the summer of 2006. Individual mode 2 packets were tracked between two moorings separated by 1 km; however, packets could not be tracked between moorings separated by greater distances from one another (∼10 km). The inability to track individual packets large distances through the mooring array combined with detailed observations from a ship suggest that these waves are short lived. The evolution of the ship‐tracked wave group was recorded using acoustic backscatter, acoustic Doppler current profilers, and turbulence profiling. The leading mode 2 wave quickly changed form and developed a tail of short, small‐amplitude mode 1 waves. The wavelength of the mode 1 oscillations agreed with that expected for a copropagating tail on the basis of linear theory. Turbulent dissipation in the mixed layer and radiation of the short mode 1 waves contributed to rapid energy loss in the leading mode 2 wave, consistent with the observed decay rate and short life span of only a few hours. The energy in the leading mode 2 wave was 10–100 times smaller than the energy of mode 1 nonlinear internal waves observed during the experiment; however, the magnitudes of wave‐localized turbulent dissipation were similar

Vertical heat flux and lateral mass transport in nonlinear internal waves

Comprehensive observations of velocity, density, and turbulent dissipation permit quantification of the nonlinear internal wave (NLIW) contribution to vertical heat flux and lateral mass transport over New Jersey’s shelf. The effect of NLIWs on the shelf heat budget was significant. On average, heat flux in NLIWs was 10 times larger than background at the pycnocline depth. NLIWs were present at midshelf <10% of the time, yet we estimate that they contributed roughly one−half the heat flux across the pycnocline during the observation period, which was characterized by weak to moderate winds. Lateral transport distances due to the leading 3 waves in NLIW packets were typically (100 m) but ranged several kilometers. The month‐averaged daily onshore transport (per unit alongshelf dimension) by NLIWs is estimated as 0.3 m2s⁻¹. This is comparable to a weak downwelling wind, but sustained over an entire month

Vertical heat flux and lateral mass transport in nonlinear internal waves

Author Posting. © American Geophysical Union, 2010. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 37 (2010): L08601, doi:10.1029/2010GL042715.Comprehensive observations of velocity, density, and turbulent dissipation permit quantification of the nonlinear internal wave (NLIW) contribution to vertical heat flux and lateral mass transport over New Jersey's shelf. The effect of NLIWs on the shelf heat budget was significant. On average, heat flux in NLIWs was 10 times larger than background at the pycnocline depth. NLIWs were present at midshelf <10% of the time, yet we estimate that they contributed roughly one−half the heat flux across the pycnocline during the observation period, which was characterized by weak to moderate winds. Lateral transport distances due to the leading 3 waves in NLIW packets were typically inline equation(100 m) but ranged several kilometers. The month-averaged daily onshore transport (per unit alongshelf dimension) by NLIWs is estimated as 0.3 m2s−1. This is comparable to a weak downwelling wind, but sustained over an entire month.This work was funded by the Office of Naval Research

Distinct Frontal Ablation Processes Drive Heterogeneous Submarine Terminus Morphology

An edited version of this paper was published by AGU. Copyright 2019 American Geophysical Union.Calving and submarine melt drive frontal ablation and sculpt the ice face of marine‐terminating glaciers. However, there are sparse observations of submarine termini, which limit estimates of spatially varying submarine melt. Here we present a detailed survey of a west Greenland glacier to reveal heterogeneity in submarine terminus morphology. We find that the majority of the terminus (~77%) is undercut, driven by calving in the upper water column and submarine melting at depth. The remaining ~23% of the terminus is overcut, driven by calving alone. We use observations of six subglacial discharge outlets, combined with a plume model, to estimate spatially varying discharge fluxes. While small discharge fluxes (<43 m3/s) feed numerous, deeply undercut outlets with subsurface plumes, ~70% of the net subglacial flux emerges at the terminus center, producing a vigorous, surface‐reaching plume. This primary outlet drives large, localized seasonal retreat that exceeds calving rates at secondary outlets

Are any coastal internal tides predictable?

Author Posting. © The Oceanography Society, 2012. This article is posted here by permission of The Oceanography Society for personal use, not for redistribution. The definitive version was published in Oceanography 25, No. 2 (2012): 80-95, doi:10.5670/oceanog.2012.44.Surface tides are the heartbeat of the ocean. Because they are controlled by Earth's motion relative to other astronomical objects in our solar system, surface tides act like clockwork and generate highly deterministic ebb and flow familiar to all mariners. In contrast, baroclinic motions at tidal frequencies are much more stochastic, owing to complexities in how these internal motions are generated and propagate. Here, we present analysis of current records from continental margins worldwide to illustrate that coastal internal tides are largely unpredictable. This conclusion has numerous implications for coastal processes, as across-shelf exchange and vertical mixing are, in many cases, strongly influenced by the internal wave field.This work was supported through grants from the US Office of Naval Research and NSF (Nash, Shroyer, Musgrave, Levine, and Duda), by NERC (grant NE/IO30224/1 FASTNEt; Inall), and by AIMS/CSIRO (Kelly and Jones)

Establishment of Highly Tumorigenic Human Colorectal Cancer Cell Line (CR4) with Properties of Putative Cancer Stem Cells

BACKGROUND: Colorectal cancer (CRC) has the third highest mortality rates among the US population. According to the most recent concept of carcinogenesis, human tumors are organized hierarchically, and the top of it is occupied by malignant stem cells (cancer stem cells, CSCs, or cancer-initiating cells, CICs), which possess unlimited self-renewal and tumor-initiating capacities and high resistance to conventional therapies. To reflect the complexity and diversity of human tumors and to provide clinically and physiologically relevant cancer models, large banks of characterized patient-derived low-passage cell lines, and especially CIC-enriched cell lines, are urgently needed. PRINCIPAL FINDINGS: Here we report the establishment of a novel CIC-enriched, highly tumorigenic and clonogenic colon cancer cell line, CR4, derived from liver metastasis. This stable cell line was established by combining 3D culturing and 2D culturing in stem cell media, subcloning of cells with particular morphology, co-culture with carcinoma associated fibroblasts (CAFs) and serial transplantation to NOD/SCID mice. Using RNA-Seq complete transcriptome profiling of the tumorigenic fraction of the CR4 cells in comparison to the bulk tumor cells, we have identified about 360 differentially expressed transcripts, many of which represent stemness, pluripotency and resistance to treatment. Majority of the established CR4 cells express common markers of stemness, including CD133, CD44, CD166, EpCAM, CD24 and Lgr5. Using immunocytochemical, FACS and western blot analyses, we have shown that a significant ratio of the CR4 cells express key markers of pluripotency markers, including Sox-2, Oct3/4 and c-Myc. Constitutive overactivation of ABC transporters and NF-kB and absence of tumor suppressors p53 and p21 may partially explain exceptional drug resistance of the CR4 cells. CONCLUSIONS: The highly tumorigenic and clonogenic CIC-enriched CR4 cell line may provide an important new tool to support the discovery of novel diagnostic and/or prognostic biomarkers as well as the development of more effective therapeutic strategies