The influence of naringin or hesperidin dietary supplementation on broiler meat quality and oxidative stability

An experiment was conducted to examine the effects of supplementing broiler feed with hesperidin or naringin, on growth performance, carcass characteristics, breast meat quality and the oxidative stability of breast and thigh meat. Two hundred and forty 1-day-old Ross 308 broiler chickens were randomly assigned to 6 groups. One of the groups served as a control (C) and was given commercial basal diets, whereas the other five groups were given the same diets further supplemented with naringin at 0.75 g/kg (N1), naringin at 1.5 g/kg (N2), hesperidin at 0.75 g/kg (E1), hesperidin at 1.5 g/kg (E2) and a-tocopheryl acetate at 0.2 g/kg (E). At 42 days of age, 10 chickens per treatment group were slaughtered for meat quality and oxidative stability assessment. No significant differences were observed among groups in final body weight, carcass weight and internal organs weights (P>0.05) apart from liver that decreased linearly with increased levels of naringin (P-linear0.05). Measurement of lipid oxidation values showed that after hesperidin and naringin dietary supplementation, malondialdehyde values decreased in tissue samples in a dose depended manner (P-linear<0.05). In conclusion, hesperidin and naringin, positively influence meat antioxidative properties without negative implications on growth performance and meat quality characteristics in poultry, thus appearing as important additives for both the consumer and the industry

Effect of Temperature and Sucrose on In Vitro Seed Germination and Bulblet Production of Pancratium maritimum L.

Sea daffodil is a perennial medicinal plant with several beneficial health properties that is enlisted as an endangered and protected species. For the exploitation and conservation of such valuable species, sustainable propagation is a key factor. For this purpose, the establishment of in vitro cultures using botanical seeds is an effective way to produce a large number of microplants which can be used as propagating material and/or bulblets with further uses for the isolation–production of biocactive substances. In this work, we performed two separate experiments where we studied (a) the effect of temperature (10 °C, 15 °C, 20 °C, 25 °C, and 30 °C) on seed germination in vitro and (b) the effect of temperature (10 °C, 20 °C, and 30 °C) and sucrose concentration (40 and 80 g/L) in growth medium on microplant development and the production of bulblets. Seed germination rates were high (over 70%) at a wide range of temperatures (15–30 °C), although the fastest and the most uniform germination was observed at 20–25 °C. Regarding the effect of temperature on microplant development and bulblet production, low temperatures (10 °C) had a negative effect on the number of leaves, bulb diameter, and fresh weight (F.W.) and dry weight (D.W.) of leaves, roots, and bulblets. The best growth of microplants (number of leaves, F.W. and D.W. of leaves, roots) and bulblet production (diameter, F.W., and D.W.) were observed in culture media with sucrose concentration 40 g/L and temperature of 30 °C. In culture medium with a sucrose concentration of 80 g/L the production of bulbs (diameter, F.W., and D.W.) was not inferior to culture media with a sucrose concentration of 40 g/L, although microplants had less root growth. It is concluded that the best treatment for the production of well-rooted microplants with large bulblets that could be used as propagating material or for the production of bulblets as a source for twin scales explants or pharmaceutical substances was the culture at 30 °C in culture medium with sucrose concentration 40 g/L. On the other hand, culture medium with a sucrose concentration of 80 g/L at 30 °C or 20 °C could be also used for the production of bulblets. © 2022 by the authors

First record of Kermes echinatus on Quercus ilex

ABSTRACT The scale insect Kermes echinatus Balachowsky (Hemiptera, Coccoidea, Kermesidae) was recorded on Quercus ilex in November 2012 in Kalamata and in December of the same year on the same host species in Athens. Quercus ilex is recorded for the first time as the host of this scale. This scale is first recorded in continental Greece. The population of the scale in both areas consisted mainly of first instar nymphs, whereas in Kalamata individuals of the scale in advanced developmental stages were recorded. Preliminary studies indicate that natural enemies are active against K. echinatus. Moultings of larvae and pupae and adults of Chilocorus bipustulatus L. (Coleoptera: Coccinellidae) were found on colonies of the scale. Parasitoid exit holes were recorded on female adults of the scale. Some data on morphology and infestation of the scale on Q. ilex are given

Effect of dietary supplementation with naringin or hesperidin on broiler body, carcass and internal organ weights at the 42nd d of age (n = 10).

<p>¹ C: no additive, E1 and E2: 0.75 and 1.5 g hesperidin per kg feed, respectively, N1 and N2: 0.75 and 1.5 g naringin per kg feed, respectively, VE: 0.2g a-tocopheryl acetate (vitamin E) per kg feed.</p><p>* P<0.05 compared with C group.</p><p>^§ P<0.05 compared with VE group.</p><p>Effect of dietary supplementation with naringin or hesperidin on broiler body, carcass and internal organ weights at the 42nd d of age (n = 10).</p

Ingredients and chemical composition of the diets used.

<p>¹ The vitamin and mineral premix provided per kg of diet: 13,000 IU of vitamin A (retinyl acetate), 5,000 IU of cholecalciferol, 80 mg of vitamin E (DL-α-tocopheryl acetate), 4 mg of menadione, 4.2 mg of thiamine, 8 mg of riboflavin, 6 mg of pyridoxin, 20 μg of cobalamin, 75 mg of nicotinic acid, 18 mg of pantothenic acid, 2 mg of folic acid, 240 μg of biotin, 10 mg of vitamin C (ascorbic acid), 500 mg of choline chloride, 0.23 mg of Co, 1.2 mg of I, 0.35 mg of Se, 50 mg of Fe, 140 mg of Mn, 25 mg of Cu, and 115 mg of Zn.</p><p>² Calculated</p><p>Ingredients and chemical composition of the diets used.</p

Effect of dietary supplementation with naringin or hesperidin on broiler <i>pectoralis major</i> instrumental quality traits (n = 10).

<p>¹ C: no additive, E1 and E2: 0.75 and 1.5 g hesperidin per kg feed, respectively, N1 and N2: 0.75 and 1.5 g naringin per kg feed, respectively, VE: 0.2g a-tocopheryl acetate (vitamin E) per kg feed.</p><p>L* = lightness.</p><p>a* = redness.</p><p>b* = yellowness.</p><p>* P<0.05 compared with C group.</p><p>^§ P<0.05 compared with VE group.</p><p>Effect of dietary supplementation with naringin or hesperidin on broiler <i>pectoralis major</i> instrumental quality traits (n = 10).</p

Effect of dietary supplementation with naringin or hesperidin on broilers’ meat (pectoralis major and biceps femoris) oxidative stability during storage (ng MDA/g meat) (n = 6).

<p>¹ C: no additive, E1 and E2: 0.75 and 1.5 g hesperidin per kg feed, respectively, N1 and N2: 0.75 and 1.5 g naringin per kg feed, respectively, VE: 0.2g a-tocopheryl acetate (vitamin E) per kg feed.</p><p>² stored for 3, 6 and 9 d at 4°C and for 120 d at -20°C.</p><p>* P<0.05 compared with C group.</p><p>^§ P<0.05 compared with VE group.</p><p>MDA, Malondialdehyde.</p><p>Effect of dietary supplementation with naringin or hesperidin on broilers’ meat (pectoralis major and biceps femoris) oxidative stability during storage (ng MDA/g meat) (n = 6).</p