2 research outputs found
Prediction of exacerbation chronic bronchopulmonary diseases in children with influenza
The objective: To develop a method for predicting exacerbation of chronic illness in children with asthma and cystic fibrosis, patients with influenza, based on the study of the dynamics of cytokines.Β Materials and methods: Were examined 52 patients with bronchial asthma and 45 children with cystic fibrosis at the age from 1 year to 12 years, located in infectious pulmonary Department at the planned treatment of underlying pathology, in which influenza was in-hospital infection. Control group observations included 40 patients with the flu, without concomitant pulmonary disease.Β The etiology of viral infection was established by detection of viral RNA in nasopharyngeal swabs by PCR. Among the influenza viruses were identified influenza ΠH1N1, ΠH3N2, influenza B, and in 2009β2010 the predominant antigen was the pandemic influenza virus ΠH1N1pdm09.Β Determination of the concentration of serum interleukins IL-1Ξ², IL-4, IL-8, IL-10, Π’NF-Ξ±, IFN-Ξ³ was performed in the 1st and 3rd day of hospitalization cytokines by the solid-phase immune-enzyme assay. Analysis of the results performed using statistical package SPSS 17.0 EN for Windows.Β Results: The flu caused the aggravation associated bronchopulmonary pathology in 2/3 of children, as MV patients, and patients with BA (65,4%-66,7%, respectively). With an increase of the ratio of IL-4 / IFN-Ξ³ and IL-10/IFN-Ξ³, at least 5-6 times, influenza can be considered a trigger of exacerbation of chronic bronchopulmonary pathologies that require amplification of the therapy of bronchial asthma and of Ρystic fibrosis. The growth of prognostic coefficients in 2-3 times allows using for treatment of influenza in these patients only antiviral agents.Β Conclusion: The study has shown a method for predicting exacerbation of bronchial asthma and cystic fibrosis in children at an early stage of influenza by calculating the ratio of IL-4/IFN-Ξ³ and IL-10/IFN-Ξ³ in children aged from 1 year to 12 years
ΠΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΡ Ρ ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ Π±ΡΠΎΠ½Ρ ΠΎΠ»Π΅ΜΠ³ΠΎΡΠ½ΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΈΜ ΠΏΡΠΈ Π³ΡΠΈΠΏΠΏΠ΅ Ρ Π΄Π΅ΡΠ΅ΠΈΜ
The objective: To develop a method for predicting exacerbation of chronic illness in children with asthma and cystic fibrosis, patients with influenza, based on the study of the dynamics of cytokines.Β Materials and methods: Were examined 52 patients with bronchial asthma and 45 children with cystic fibrosis at the age from 1 year to 12 years, located in infectious pulmonary Department at the planned treatment of underlying pathology, in which influenza was in-hospital infection. Control group observations included 40 patients with the flu, without concomitant pulmonary disease.Β The etiology of viral infection was established by detection of viral RNA in nasopharyngeal swabs by PCR. Among the influenza viruses were identified influenza ΠH1N1, ΠH3N2, influenza B, and in 2009β2010 the predominant antigen was the pandemic influenza virus ΠH1N1pdm09.Β Determination of the concentration of serum interleukins IL-1Ξ², IL-4, IL-8, IL-10, Π’NF-Ξ±, IFN-Ξ³ was performed in the 1st and 3rd day of hospitalization cytokines by the solid-phase immune-enzyme assay. Analysis of the results performed using statistical package SPSS 17.0 EN for Windows.Β Results: The flu caused the aggravation associated bronchopulmonary pathology in 2/3 of children, as MV patients, and patients with BA (65,4%-66,7%, respectively). With an increase of the ratio of IL-4 / IFN-Ξ³ and IL-10/IFN-Ξ³, at least 5-6 times, influenza can be considered a trigger of exacerbation of chronic bronchopulmonary pathologies that require amplification of the therapy of bronchial asthma and of Ρystic fibrosis. The growth of prognostic coefficients in 2-3 times allows using for treatment of influenza in these patients only antiviral agents.Β Conclusion: The study has shown a method for predicting exacerbation of bronchial asthma and cystic fibrosis in children at an early stage of influenza by calculating the ratio of IL-4/IFN-Ξ³ and IL-10/IFN-Ξ³ in children aged from 1 year to 12 years.Β Π¦Π΅Π»Ρ: ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°ΡΡ ΡΠΏΠΎΡΠΎΠ± ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΡ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ ΠΏΡΠΈ Π³ΡΠΈΠΏΠΏΠ΅ Ρ Π΄Π΅ΡΠ΅ΠΈΜ, Π±ΠΎΠ»ΡΠ½ΡΡ
Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΈΜ Π°ΡΡΠΌΠΎΠΈΜ ΠΈ ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·ΠΎΠΌ, Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ².Β ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Ρ 52 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ° Ρ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΈΜ Π°ΡΡΠΌΠΎΠΈΜ ΠΈ 45 Π΄Π΅ΡΠ΅ΠΈΜ Ρ ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·ΠΎΠΌ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 1 Π³ΠΎΠ΄Π° Π΄ΠΎ 12 Π»Π΅Ρ, Π½Π°Ρ
ΠΎΠ΄ΠΈΠ²ΡΠΈΡ
ΡΡ Π² ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎ-ΠΏΡΠ»ΡΠΌΠΎΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΎΡΠ΄Π΅Π»Π΅Π½ΠΈΠΈ Π½Π° ΠΏΠ»Π°Π½ΠΎΠ²ΠΎΠΌ Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΈΜ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ, Ρ ΠΊΠΎΡΠΎΡΡΡ
Π³ΡΠΈΠΏΠΏ ΡΠ²Π»ΡΠ»ΡΡ Π³ΠΎΡΠΏΠΈΡΠ°Π»ΡΠ½ΠΎΠΈΜ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΈΜ. ΠΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΡ Π³ΡΡΠΏΠΏΡ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠΈΜ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ 40 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π³ΡΠΈΠΏΠΏΠΎΠΌ, Π½ΠΎ Π±Π΅Π· ΡΠΎΠΏΡΡΡΡΠ²ΡΡΡΠΈΡ
Π±ΡΠΎΠ½Ρ
ΠΎΠ»Π΅Π³ΠΎΡΠ½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΈΜ. ΠΡΠΈΠΎΠ»ΠΎΠ³ΠΈΡ Π²ΠΈΡΡΡΠ½ΠΎΠΈΜ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ ΡΡΡΠ°Π½Π°Π²Π»ΠΈΠ²Π°Π»Π°ΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ΠΌ Π²ΠΈΡΡΡ-ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΈΜ Π ΠΠ Π² Π½ΠΎΡΠΎΠ³Π»ΠΎΡΠΎΡΠ½ΡΡ
ΡΠΌΡΠ²Π°Ρ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π . Π‘ΡΠ΅Π΄ΠΈ Π²ΠΈΡΡΡΠΎΠ² Π³ΡΠΈΠΏΠΏΠ° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈΡΡ ΠH1N1, ΠH3N2, Π, Π° Π² 2009β2010 Π³Π³. ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠΌ Π°Π½ΡΠΈΠ³Π΅Π½ΠΎΠΌ ΡΠ²Π»ΡΠ»ΡΡ Π²ΠΈΡΡΡ ΠΏΠ°Π½Π΄Π΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π³ΡΠΈΠΏΠΏΠ° ΠH1N1pdm09.Β ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΈΠ½ΡΠ΅ΡΠ»Π΅ΠΈΜΠΊΠΈΠ½ΠΎΠ² IL-1Ξ², IL-4, IL-8, IL-10, Π’NF-Ξ±, IFN-Ξ³ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ Π² 1-ΠΈΜ ΠΈ 3-ΠΈΜ Π΄Π΅Π½Ρ Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Β«ΡΡΠ½Π΄Π²ΠΈΡΒ»-ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ²Π΅ΡΠ΄ΠΎΡΠ°Π·Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°. Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ SPSS 17.0 RU for Windows.Β Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π³ΡΠΈΠΏΠΏ Π²ΡΠ·ΡΠ²Π°Π» ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠΏΡΡΡΡΠ²ΡΡΡΠ΅ΠΈΜ Π±ΡΠΎΠ½Ρ
ΠΎΠ»Π΅Π³ΠΎΡΠ½ΠΎΠΈΜ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Ρ Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π° Π΄Π΅ΡΠ΅ΠΈΜ, Π±ΠΎΠ»ΡΠ½ΡΡ
ΠΊΠ°ΠΊ ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·ΠΎΠΌ, ΡΠ°ΠΊ ΠΈ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΈΜ Π°ΡΡΠΌΠΎΠΈΜ (65,4% ΠΈ 66,7% ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ). ΠΡΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠΈ ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΠ° ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ IL-4/IFN-Ξ³ ΠΈ IL-10/IFN-Ξ³ ΠΊΠ°ΠΊ ΠΌΠΈΠ½ΠΈΠΌΡΠΌ Π² 5β6 ΡΠ°Π· Π³ΡΠΈΠΏΠΏ ΠΌΠΎΠΆΠ΅Ρ ΡΡΠΈΡΠ°ΡΡΡΡ ΡΡΠΈΠ³Π³Π΅ΡΠΎΠΌ ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΡ ΡΠΎΠΏΡΡΡΡΠ²ΡΡΡΠ΅ΠΈΜ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΈΜ Π±ΡΠΎΠ½Ρ
ΠΎΠ»Π΅Π³ΠΎΡΠ½ΠΎΠΈΜ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ. ΠΠ°ΡΠ°ΡΡΠ°Π½ΠΈΠ΅ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΠΎΠ² Π² 2β3 ΡΠ°Π·Π° ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΎΠ³ΡΠ°Π½ΠΈΡΠΈΡΡ Π»Π΅ΡΠ΅Π½ΠΈΠ΅ Π³ΡΠΈΠΏΠΏΠ° Ρ Π΄Π°Π½Π½ΠΎΠΈΜ ΠΊΠ°ΡΠ΅Π³ΠΎΡΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΈΡΡΡΠ½ΡΠΌΠΈ ΡΡΠ΅Π΄ΡΡΠ²Π°ΠΌΠΈ.Β ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅: ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ ΡΠΏΠΎΡΠΎΠ± ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ±ΠΎΡΡΡΠ΅Π½ΠΈΡ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΈΜ Π°ΡΡΠΌΡ ΠΈ ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·Π° Ρ Π΄Π΅ΡΠ΅ΠΈΜ Π½Π° ΡΠ°Π½Π½Π΅ΠΈΜ ΡΡΠ°Π΄ΠΈΠΈ Π³ΡΠΈΠΏΠΏΠ° Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ°ΡΡΠ΅ΡΠ° ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΠΎΠ² ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ IL-4/IFN-Ξ³ ΠΈ IL-10/IFN-Ξ³ Ρ Π΄Π΅ΡΠ΅ΠΈΜ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 1 Π³ΠΎΠ΄Π° Π΄ΠΎ 12 Π»Π΅Ρ.