6 research outputs found
The combination of electron capture dissociation and fixed charge derivatization increases sequence coverage for O-glycosylated and O-phosphorylated peptides
Do soothing vocal cues enhance horses' ability to learn a frightening task?
When working with horses, it is frequently asserted that horses have an inherent understanding of harsh voice cues that would be used as reprimands versus soothing voice cues that may be used as positive reinforcers or calming modifiers. If horses are unable to understand this difference while their handlers assume they can, it may potentially lead to unfair or inappropriate training. A total of 107 horses from 2 different horse facilities in the United States and 7 different horse facilities in Europe were randomly assigned to either soothing voice treatment (SV; n = 58) or harsh voice treatment (HV; n = 49). The learning task involved horses of various breeds and ages learning to cross a tarpaulin. Methodology was standardized across locations. SV involved handlers saying \u201cgood horse\u201c in a soft soothing manner whenever horses made forward progress toward the tarpaulin. HV involved saying \u201cquit it\u201c in a loud harsh manner whenever horses made forward progress toward the tarpaulin. Praat software was used to assess similarities in vocal spectrograms and acoustic parameters of different handlers and treatments. Mean pitch for SV and HV was 236.2 \ub1 2.2 Hz and 322.1 \ub1 8.9 Hz, respectively, both well within the equine hearing range and different at P < 0.001. Average intensity (loudness) for SV and HV was 51.2 \ub1 1.7 dB and 61.7 \ub1 1.2, respectively, different at P < 0.001. Contrary to our hypotheses, risk of failing the task (>10 minutes to cross the tarpaulin for the first time) was not different between treatments (22.4% failures on SV; 24.5% failures on HV; P = 0.41). Also, for those horses who did cross the tarpaulin, the total time to achieve the calmness criterion (crossing with little or no obvious anxiety) did not differ between treatments (139.9 \ub1 50.4 for HV vs. 241.6 \ub1 40.3 for SV; P = 0.25). There was no difference between the average heart rate (HR; n = 70 horses) of horses that crossed (82.9 \ub1 7.0 beats/minute) versus those that failed (77.4 \ub1 6.7; P = 0.43). Also, there was no difference between the average HR of HV horses (85.7 \ub1 3.9 beats/minute) versus SV horses (77.9 \ub1 3.7 beats/minute; P = 0.16). Furthermore, there was no difference between the maximum HRs, with HV horses registering a mean of 143.4 \ub1 11.25 beats/minute and SV horses registering a mean of 166.1 \ub1 9.5 beats/minute; P = 0.20. In the context of this study, soothing vocal cues did not enhance horses' ability to perform a novel potentially frightening task
Do horses recognize the difference between harsh tones and soothing tones when using voice as a reinforcer for learning a frightening task
When working with horses, it is frequently asserted that horses have an inherent understanding of harsh voice cues that would be used as reprimands versus soothing voice cues that may be used as positive reinforcers/calming modifiers. If horses are unable to understand this difference, handlers often make poor assumptions that potentially lead to unfair training. A total of 95 horses from 4 different locations in US and Europe were randomly assigned to either soothing voice treatment (SV; n=52) or harsh voice treatment (HV; n=43). The learning task involved horses of various breeds and ages learning to cross a tarpaulin. Methodology was standardised across locations. SV involved handlers saying \u201cgood horse\u201d in a soft, soothing manner whenever horses made forward progress toward the tarpaulin. HV involved saying \u201cquit it\u201d in a loud, harsh manner whenever horses made forward progress toward the tarpaulin. PRAAT software was used to assess similarities in vocal spectrograms of different handlers/treatments. Mean pitch for SV was 244.4\ub13.11 Hz and 275.1\ub12.01 Hz for HV; both well within the equine hearing range. Average intensity (loudness) for SV was 42.3\ub11.04 dB and 56.0\ub11.80 for HV. Contrary to our hypotheses, risk of failing the task (> 10 min to cross the tarpaulin for the 1st time) was not different between treatments (25% failures SV; 25.5% failures HV; p=0.55). Also, for those horses who did cross the tarpaulin, the total time to achieve calmness criterion (crossing with little/no obvious anxiety) did not differ between treatments (157.3\ub159.8 sec HV vs 245.8\ub143.5 SV, p=0.23. A breed difference was noted: Hot bloods=606.8\ub1145.9 sec vs Warm bloods=120.7\ub118.3 sec, p<0.01. Polar heart rate monitors were used on 68 horses. There was no difference between average HR of horses who crossed (84.7\ub13.9 bpm) vs those who failed (82.1\ub15.1) p=0.69. There was also no difference between HV horses (85.4\ub14.8 bpm) and SV horses (81.3\ub14.2) p=0.52