The ecology of microorganisms in a small closed system: Potential benefits and problems for space station

The inevitble presence on the space station of microorganisms associated with crew members and their environment will have the potential for both benefits and a range of problems including illness and corrosion of materials. This report reviews the literature presenting information about microorganisms pertinent to Environmental Control and Life Support (ECLS) on the space station. The perspective of the report is ecological, viewing the space station as an ecosystem in which biological relationships are affected by factors such as zero gravity and by closure of a small volume of space. Potential sites and activities of microorganisms on the space station and their environmental limits, microbial standards for the space station, monitoring and control methods, effects of space factors on microorganisms, and extraterrestrial contamination are discussed

Comparison of epifluorescent viable bacterial count methods

Two methods, the 2-(4-Iodophenyl) 3-(4-nitrophenyl) 5-phenyltetrazolium chloride (INT) method and the direct viable count (DVC), were tested and compared for their efficiency for the determination of the viability of bacterial populations. Use of the INT method results in the formation of a dark spot within each respiring cell. The DVC method results in elongation or swelling of growing cells that are rendered incapable of cell division. Although both methods are subjective and can result in false positive results, the DVC method is best suited to analysis of waters in which the number of different types of organisms present in the same sample is assumed to be small, such as processed waters. The advantages and disadvantages of each method are discussed

Topical cyclone rainfall characteristics as determined from a satellite passive microwave radiometer

Data from the Nimbus-5 Electrically Scanning Microwave Radiometer (ESMR-5) were used to calculate latent heat release and other rainfall parameters for over 70 satellite observations of 21 tropical cyclones in the tropical North Pacific Ocean. The results indicate that the ESMR-5 measurements can be useful in determining the rainfall characteristics of these storms and appear to be potentially useful in monitoring as well as predicting their intensity. The ESMR-5 derived total tropical cyclone rainfall estimates agree favorably with previous estimates for both the disturbance and typhoon stages. The mean typhoon rainfall rate (1.9 mm h(-1)) is approximately twice that of disturbances (1.1 mm h(-1))

Monitoring tropical cyclone intensity using wind fields derived from short-interval satellite images

Rapid scan visible images from the Visible Infrared Spin Scan Radiometer sensor on board SMS-2 and GOES-1 were used to derive high resolution upper and lower tropospheric environmental wind fields around three western Atlantic tropical cyclones (1975-78). These wind fields were used to derive upper and lower tropospheric areal mean relative vorticity and their differences, the net relative angular momentum balance and upper tropospheric mass outflow. These kinematic parameters were shown by studies using composite rawinsonde data to be strongly related to tropical cyclone formation and intensity changes. Also, the role of forced synoptic scale subsidence in tropical cyclone formation was examined. The studies showed that satellite-derived lower and upper tropospheric wind fields can be used to monitor and possibly predict tropical cyclone formation and intensity changes. These kinematic analyses showed that future changes in tropical cyclone intensity are mainly related to the "spin-up" of the storms by the net horizontal transport of relative angular momentum caused by convergence of cyclonic vorticity in the lower troposphere and to a lesser extent the divergence of anticyclone vorticity in the upper troposphere

Satellite-observed latent heat release in a tropical cyclone

Data from the Nimbus 5 electrically scanning microwave radiometer (ESMR) are used to make calculations of the latent heat release (L.H.R.) and the distribution of rainfall rate in a tropical cyclone as it grows from a tropical disturbance to a typhoon. The L.H.R. (calculated over a circular area of 4 deg latitude radius) increases during the development and intensification of the storm from a magnitude of 2.7 X 10 to the 21st power ergs/s (in the disturbance stage) to 8.8 X 10 to the 21st power ergs (typhoon stage). The latter value corresponds to a mean rainfall rate of 2.0 mm hr/s. The more intense the cyclone and the greater the L.H.R., the greater the percentage contribution of the larger rainfall rates to the L.H.R. In the disturbance stage the percentage contribution of rainfall rates less than or minus 6 mm hr/s is typically 8%; for the typhoon stage, the value is 38%. The distribution of rainfall rate as a function of radial distance from the center indicates that as the cyclone intensifies, the higher rainfall rates tend to concentrate toward the center of the circulation

An objective method for forecasting tropical cyclone intensity and motion using Nimbus-5 ESMR measurements and non-satellite derived descriptors

An empirical analysis program, based on finding an optimal representation of the data was applied to 120 observations of 29 1973 and 1974 North Pacific tropical cyclones. Each observation consists of a field of Nimbus-5 Electrically Scanning Microwave Radiometer radiation measurements at 267 grid points covering and surrounding the tropical cyclone plus nine other non-satellite derived discriptors. Forecast algorithms to estimate storm intensity and motion at 12, 24, 48, and 72 hours after each observation were developed using an independent eigen screening analysis. These algorithms were based on best track data. Independent testing of these algorithms showed that the performance of most of these algorithms were better than persistence and the algorithms forecasting 24, 48, and 72 hour maximum wind speed were better than those made operationally by the Joint Typhoon Warning Center for 1973 and 1974 that did not use best track data

The role of the equivalent blackbody temperature in the study of Atlantic Ocean tropical cyclones

Satellite measured equivalent blackbody temperatures of Atlantic Ocean tropical cyclones are used to investigate their role in describing the convection and cloud patterns of the storms and in predicting wind intensity. The high temporal resolution of the equivalent blackbody temperature measurements afforded with the geosynchronous satellite provided sequential quantitative measurements of the tropical cyclone which reveal a diurnal pattern of convection at the inner core during the early developmental stage; a diurnal pattern of cloudiness in the storm's outer circulation throughout the life cycle; a semidiurnal pattern of cloudiness in the environmental atmosphere surrounding the storms during the weak storm stage; an outward modulating atmospheric wave originating at the inner core; and long term convective bursts at the inner core prior to wind intensification

Global distribution of total ozone derived from Nimbus 3 satellite during April July, 1969 and its implication to upper tropospheric circulation

Utilizing a stepwise multiple regression scheme, monthly mean global maps of total ozone are produced from the spectral intensity measurements made by the infrared interferometer spectrometer (IRIS) onboard the Nimbus 3 satellite for the period April 18 to July 22, 1969. These maps show that over the equatorial regions total ozone increases steadily from April to July. Further, latitudinal variation of total ozone derived from these maps shows that the ozone increases at all latitudes over the Southern Hemisphere uniformly from April to July while over the Northern Hemisphere the monthly decrease at all latitudes is nonuniform. In general, these maps bear a close resemblance to the upper tropospheric circulation. It is observed that the total ozone is best correlated with the 200 mb geopotential heights. This relationship enables the utilization of total ozone as a quasi-stream function to determine geostrophic winds at 200 mb level. Further, even at low latitudes where the total ozone does not bear simple relationship to the geopotential heights the course of the easterly jet stream with the help of the ozone measurements can be identified

Analysis of debris from Spacelab Space Life Sciences-1

Airborne microbiological and particulate contamination generated aboard Spacelab modules is a potential safety hazard. In order to shed light on the characteristics of these contaminants, microbial and chemical/particulate analyses were performed on debris vacuumed from cabin and avionics air filters in the Space Life Sciences-1 (SLS-1) module of the Space Transportation System 40 (STS-40) mission 1 month after landing. The debris was sorted into categories (e.g., metal, nonmetal, hair/fur, synthetic fibers, food particles, insect fragments, etc.). Elemental analysis of particles was done by energy dispersive analysis of x rays (metals) and Fourier transform infrared spectroscopy (nonmetals). Scanning electron micrographs were done of most particles. Microbiological samples were grown on R2A culture medium and identified. Clothing fibers dominated the debris by volume. Other particles, all attributed to the crew, resulted from abrasions and impacts during missions operations (e.g., paint chips, plastic, electronic scraps and clothing fibers). All bacterial species identified are commonly found in the atmosphere or on the human body. Bacillus sp. was the most frequently seen bacterium. One of the bacterial species, Enterobacter agglomerans, could cause illness in crew members with depressed immune systems

Microbial biofilm studies of the environmental control and life support system water recovery test for Space Station Freedom

NASA is developing a water recovery system (WRS) for Space Station Freedom to reclaim human waste water for reuse by astronauts as hygiene or potable water. A water recovery test (WRT) currently in progress investigates the performance of a prototype of the WRS. Analysis of biofilm accumulation, the potential for microbially influenced corrosion (MIC) in the WRT, and studies of iodine disinfection of biofilm are reported. Analysis of WRT components indicated the presence of organic deposits and biofilms in selected tubing. Water samples for the WRT contained acid-producing and sulfate-reducing organisms implicated in corrosion processes. Corrosion of an aluminum alloy was accelerated in the presence of these water samples; however, stainless steel corrosion rates were not accelerated. Biofilm iodine sensitivity tests using an experimental laboratory scale recycled water system containing a microbial check valve (MCV) demonstrated that an iodine concentration of 1 to 2 mg/L was ineffective in eliminating microbial biofilm. For complete disinfection, an initial concentration of 16 mg/L was required, which was gradually reduced by the MCV over 4 to 8 hours to 1 to 2 mg/L. This treatment may be useful in controlling biofilm formation