Genome-wide signatures of population bottlenecks and diversifying selection in European wolves

Genomic resources developed for domesticated species provide powerful tools for studying the evolutionary history of their wild relatives. Here we use 61K single-nucleotide polymorphisms (SNPs) evenly spaced throughout the canine nuclear genome to analyse evolutionary relationships among the three largest European populations of grey wolves in comparison with other populations worldwide, and investigate genome-wide effects of demographic bottlenecks and signatures of selection. European wolves have a discontinuous range, with large and connected populations in Eastern Europe and relatively smaller, isolated populations in Italy and the Iberian Peninsula. Our results suggest a continuous decline in wolf numbers in Europe since the Late Pleistocene, and long-term isolation and bottlenecks in the Italian and Iberian populations following their divergence from the Eastern European population. The Italian and Iberian populations have low genetic variability and high linkage disequilibrium, but relatively few autozygous segments across the genome. This last characteristic clearly distinguishes them from populations that underwent recent drastic demographic declines or founder events, and implies long-term bottlenecks in these two populations. Although genetic drift due to spatial isolation and bottlenecks seems to be a major evolutionary force diversifying the European populations, we detected 35 loci that are putatively under diversifying selection. Two of these loci flank the canine platelet-derived growth factor gene, which affects bone growth and may influence differences in body size between wolf populations. This study demonstrates the power of population genomics for identifying genetic signals of demographic bottlenecks and detecting signatures of directional selection in bottlenecked populations, despite their low background variability.Heredity advance online publication, 18 December 2013; doi:10.1038/hdy.2013.122

The Serendipitous Discovery of a Group or Cluster of young Galaxies at z=2.40 in Deep Hubble Space Telescope WFPC2 Images

We report the serendipitous discovery of a group or cluster of young galaxies at $z\simeq$2.40 in a 24-orbit HST/WFPC2 exposure of the field around the weak radio galaxy 53W002. Potential cluster members were identified on ground-based narrow-band redshifted Ly$\alpha$ images and confirmed via spectroscopy. In addition to the known weak radio galaxy 53W002 at z=2.390, two other objects were found to have excess narrow-band Ly$\alpha$ emission at $z\simeq$2.40. Both have been spectroscopically confirmed, and one clearly contains a weak AGN. They are located within one arcminute of 53W002, or $\sim0.23h_{100}^{-1}$Mpc ($q_o$=0.5) at $z\simeq$2.40, which is the physical scale of a group or small cluster of galaxies. Profile fitting of the WFPC2 images shows that the objects are very compact, with scale lengths $\simeq$0\farcs 1 ($\simeq0.39h_{100}^{-1}$kpc), and are rather faint (luminosities < L*), implying that they may be sub-galactic sized objects. We discuss these results in the context of galaxy and cluster evolution and the role that weak AGN may play in the formation of young galaxies.Comment: Accepted for publication in The Astrophysical Journal (Letters). 13 pages of gzip compressed and uuencoded PS. Figures are available at http://www.phys.unsw.edu.au/~spd/bib.htm

The Top Ten List of Gravitational Lens Candidates from the HST Medium Deep Survey

A total of 10 good candidates for gravitational lensing have been discovered in the WFPC2 images from the HST Medium Deep Survey (MDS) and archival primary observations. These candidate lenses are unique HST discoveries, i.e. they are faint systems with sub-arcsecond separations between the lensing objects and the lensed source images. Most of them are difficult objects for ground-based spectroscopic confirmation or for measurement of the lens and source redshifts. Seven are ``strong lens'' candidates which appear to have multiple images of the source. Three are cases where the single image of the source galaxy has been significantly distorted into an arc. The first two quadruply lensed candidates were reported in Ratnatunga et al 1995 (ApJL, 453, L5) We report on the subsequent eight candidates and describe them with simple models based on the assumption of singular isothermal potentials. Residuals from the simple models for some of the candidates indicate that a more complex model for the potential will probably be required to explain the full structural detail of the observations once they are confirmed to be lenses. We also discuss the effective survey area which was searched for these candidate lens objects.Comment: 26 pages including 12 figures and 10 tables. AJ Vol. 117, No.

Primary reading exercises for use with the Durrell Analysis of Reading Difficulty

Thesis (Ed.M.)--Boston Universit

Canine Morphology: Hunting for Genes and Tracking Mutations

In this essay, Abigail Shearin and Elaine Ostrander discuss the proposed genomic mechanisms for the extraordinary level of phenotypic variation observed in the domestic dog and the evidence detailing the variants responsible for the many shapes, sizes, textures, and colors of man's best friend

Widespread, long-term admixture between grey wolves and domestic dogs across Eurasia and its implications for the conservation status of hybrids

Hybridisation between a domesticated species and its wild ancestor is an important conservation problem, especially if it results in the introgression of domestic gene variants into wild species. Nevertheless, the legal status of hybrids remains unregulated, partially because of the limited understanding of the hybridisation process and its consequences. The occurrence of hybridisation between grey wolves and domestic dogs is well-documented from different parts of the wolf geographic range, but little is known about the frequency of hybridisation events, their causes and the genetic impact on wolf populations. We analysed 61K SNPs spanning the canid genome in wolves from across Eurasia and North America and compared that data to similar data from dogs to identify signatures of admixture. The haplotype block analysis, which included 38 autosomes and the X chromosome, indicated the presence of individuals of mixed wolf-dog ancestry in most Eurasian wolf populations, but less admixture was present in North American populations. We found evidence for male-biased introgression of dog alleles into wolf populations, but also identified a first-generation hybrid resulting from mating between a female dog and a male wolf. We found small blocks of dog ancestry in the genomes of 62% Eurasian wolves studied and melanistic individuals with no signs of recent admixed ancestry, but with a dog-derived allele at a locus linked to melanism. Consequently, these results suggest that hybridisation has been occurring in different parts of Eurasia on multiple timescales and is not solely a recent phenomenon. Nevertheless, wolf populations have maintained genetic differentiation from dogs, suggesting that hybridisation at a low frequency does not diminish distinctiveness of the wolf gene pool. However, increased hybridisation frequency may be detrimental for wolf populations, stressing the need for genetic monitoring to assess the frequency and distribution of individuals resulting from recent admixture

The morphological mix of field galaxies to I=24.25 magnitudes (b=26 magnitudes) from a deep Hubble Space Telescope WFPC2 image

We determine the morphological mix of field galaxies down to $m_{I}\simeq 24.25$ mag ($m_{B}\sim 26.0$ mag) from a single ultradeep HST WFPC2 image in both the $V_{606}$ and $I_{814}$ filters. In total, we find 227 objects with $m_{I}\le 24.5$ mag and classify these into three types: ellipticals (16%), early-type spirals (37%) and late-type spirals/Irregulars (47%). The differential number counts for each type are compared to simple models in a standard flat cosmology. We find that both the elliptical and early-type spiral number counts are well described by {\it little or no}-evolution models, but only when normalized at $b_{J} = 18.0$ mag. Given the uncertainties in the luminosity function (LF) normalization, both populations are consistent with a mild evolutionary scenario based on a normal/low rate of star-formation. This constrains the end of the last {\it major} star-formation epoch in the giant galaxy populations to $z\geq 0.8$. Conversely, the density of the observed late-type/Irregular population is found to be a factor of 10 in excess of the conventional no-evolution model. This large population might be explained by either a modified {\it local} dwarf-rich LF, and/or strong evolution acting on the {\it local} LF. For the dwarf-rich case, a {\it steep} faint-end Schechter-slope ($\alpha\simeq -1.8$) is required plus a five-fold increase in the dwarf normalization. For a purely evolving model based on a {\it flat} Loveday {\it et al.} (1992) LF ($\alpha\simeq -1.0$), a ubiquitous starburst of $\Delta I\sim$2.0 mag is needed at z$\simeq 0.5$ for the {\it entire} late-type population. We argue for a combination of these possibilities, and show that for a steep Marzke {\it et al.} (1994) LF ($\alpha\simeq -1.5$), a starburst of $\sim$ 1.3 mag is requiredComment: 9 pages, 3 figures (2 colour). The figures are available at http://www.phys.unsw.edu.au/~spd/bib.htm

Turmeric and Its Major Compound Curcumin on Health: Bioactive Effects and Safety Profiles for Food, Pharmaceutical, Biotechnological and Medicinal Applications

Curcumin, a yellow polyphenolic pigment from the Curcuma longa L. (turmeric) rhizome, has been used for centuries for culinary and food coloring purposes, and as an ingredient for various medicinal preparations, widely used in Ayurveda and Chinese medicine. In recent decades, their biological activities have been extensively studied. Thus, this review aims to offer an in-depth discussion of curcumin applications for food and biotechnological industries, and on health promotion and disease prevention, with particular emphasis on its antioxidant, anti-inflammatory, neuroprotective, anticancer, hepatoprotective, and cardioprotective effects. Bioavailability, bioefficacy and safety features, side effects, and quality parameters of curcumin are also addressed. Finally, curcumin’s multidimensional applications, food attractiveness optimization, agro-industrial procedures to offset its instability and low bioavailability, health concerns, and upcoming strategies for clinical application are also covered