Cl,Br,CrO4 QUATERNARY RECIPROCAL SYSTEM

Partition a quaternary reciprocal system Li,KCl,Br,CrO4 into simplexes was performed. Formation of a continuous series of solid solutions was detected by the differential thermal analysis in the secant and stable elements of the system

Comparative evaluation of heart rate variability after septoplasty and tonsillectomy

The aim of the research was to determine the fluctuations of the autonomic nervous system based on the analysis of changes in certain heart rate variability (HRV) at patients after septoplasty and tonsillectomy. A study of Holter monitoring of 28 patients with nasal septum deviation and of 30 patients with chronic tonsillitis was conducted. Surgical procedures were performed under local infiltration anesthesia with 0,5% novocaine solution. We evaluated standard deviation of RR intervals (SDNN) - 24-hour monitoring, day and night data. After septoplasty for night and day validity period average SDNN did not differ (p < 0,05), but SDNN were significantly different (p < 0,05). Average values for the night and day were different from each other at the patients after tonsillectomy (p < 0,05). SDNN scatter was the same. SDNN in both groups did not differ per day both for average values and for variability (p < 0,05). Septoplasty provokes more pronounced stress response as compared with tonsillectomy. Local anesthesia for septoplasty and tonsillectomy causes a disturbance of adaptive reactions on the part of the autonomic nervous system. HRV analysis can be called an objective and individual method of diagnosis of acute stress conditions in the otorhinolaryngological surgery

SPECIES AND GENETIC VARIETY OF TICK INFECTIONS PATHOGENS ON THE TERRITORY OF THE EASTERN SIBERIA

The article sums up long-term, researches of studying of species and genetic variety of tick infections pathogens in combined, natural focuses of the Eastern Siberia. The results of the research testify to the high genetic variety of regional population, of tick-borne encephalitis virus that is represented, by the strains of Far-Eastern, West, Ural-Siberian genotypes, the strains of the group 886 and. 178-79. Infection of Ixodidae by the next pathogens: B. garinii, B. afzelii, R. sibirica, R. raoultii (генотипы. R. sp. DnS14, R. sp. DnS28), E. muris, A. phagocytophilum и Candidatus «Neoehrlichia mikurensis» was determined

Chromosomal radiosensitivity and acute radiation side effects after radiotherapy in tumour patients - a follow-up study

Radiotherapists are highly interested in optimizing doses especially for patients who tend to suffer from side effects of radiotherapy (RT). It seems to be helpful to identify radiosensitive individuals before RT. Thus we examined aberrations in FISH painted chromosomes in in vitro irradiated blood samples of a group of patients suffering from breast cancer. In parallel, a follow-up of side effects in these patients was registered and compared to detected chromosome aberrations. METHODS: Blood samples (taken before radiotherapy) were irradiated in vitro with 3 Gy X-rays and analysed by FISH-painting to obtain aberration frequencies of first cycle metaphases for each patient. Aberration frequencies were analysed statistically to identify individuals with an elevated or reduced radiation response. Clinical data of patients have been recorded in parallel to gain knowledge on acute side effects of radiotherapy. RESULTS: Eight patients with a significantly elevated or reduced aberration yield were identified by use of a t-test criterion. A comparison with clinical side effects revealed that among patients with elevated aberration yields one exhibited a higher degree of acute toxicity and two patients a premature onset of skin reaction already after a cumulative dose of only 10 Gy. A significant relationship existed between translocations in vitro and the time dependent occurrence of side effects of the skin during the therapy period. CONCLUSIONS: The results suggest that translocations can be used as a test to identify individuals with a potentially elevated radiosensitivity

Real-Time Imaging of HIF-1α Stabilization and Degradation

HIF-1α is overexpressed in many human cancers compared to normal tissues due to the interaction of a multiplicity of factors and pathways that reflect specific genetic alterations and extracellular stimuli. We developed two HIF-1α chimeric reporter systems, HIF-1α/FLuc and HIF-1α(ΔODDD)/FLuc, to investigate the tightly controlled level of HIF-1α protein in normal (NIH3T3 and HEK293) and glioma (U87) cells. These reporter systems provided an opportunity to investigate the degradation of HIF-1α in different cell lines, both in culture and in xenografts. Using immunofluorescence microscopy, we observed different patterns of subcellular localization of HIF-1α/FLuc fusion protein between normal cells and cancer cells; similar differences were observed for HIF-1α in non-transduced, wild-type cells. A dynamic cytoplasmic-nuclear exchange of the fusion protein and HIF-1α was observed in NIH3T3 and HEK293 cells under different conditions (normoxia, CoCl2 treatment and hypoxia). In contrast, U87 cells showed a more persistent nuclear localization pattern that was less affected by different growing conditions. Employing a kinetic model for protein degradation, we were able to distinguish two components of HIF-1α/FLuc protein degradation and quantify the half-life of HIF-1α fusion proteins. The rapid clearance component (t1/2 ∼4–6 min) was abolished by the hypoxia-mimetic CoCl2, MG132 treatment and deletion of ODD domain, and reflects the oxygen/VHL-dependent degradation pathway. The slow clearance component (t1/2 ∼200 min) is consistent with other unidentified non-oxygen/VHL-dependent degradation pathways. Overall, the continuous bioluminescence readout of HIF-1α/FLuc stabilization in vitro and in vivo will facilitate the development and validation of therapeutics that affect the stability and accumulation of HIF-1α

Evaluation of autonomic nervous system in patients with chronic rhinosinusitis and olfactory disorder

Studied cardiointervalography of 45 men with chronic rhinosinusitis and impaired olfactory function. In the studied group of patients predominance sympathotony was revealed. It is also shown that the detection of the initial state of the autonomic nervous system plays a role in non-pharmacological treatment of chronic rhinosinusitis with with impaired peripheral olfactory analyzer

Features of coxitis treatment in patients with ankylosing spondylitis

The hip joint damage (HJD) coxitis is a characteristic manifestation of ankylosing spondylitis (AS). Treatment policy for coxitis in this disease has not been worked out today. There are a few studies evaluating the efficacy of tumor necrosis factor-α (IFN-α ) inhibitors in these patients. Coxitis treatment with disease-modifying antirheumatic drugs (DMARDs) is under study.The paper describes three clinical cases of patients with AS and coxitis, in two of whom combined therapy with nonsteroidal anti-inflammatory drugs (NSAIDs), DMARDs, and IFN-α inhibitors produced a fairly rapid positive effect in reducing inflammatory changes in HJD as evidenced by magnetic resonance imaging; moreover, there was no x-ray progression. It can be assumed that there is a so-called window of opportunity when no irreversible structural changes in HJD occur during timely initiated therapy with biological agents (BAs).Our findings suggest that further investigations are needed to clarify the effects of DMARD and combination therapy using DMARD and BAs in combination with NSAIDs

THE EVOLUTION OF AXIAL SPONDYLOARTHRITIS DURING 12-MONTH FOLLOW-UP STUDY OF A CORSAR COHORT

Until recently there is no clarity in the nosological independence of non-radiographic axial spondyloarthritis  (nraxSpA). Cohort studies can assist in solving this problem. In 2013, the V.A. Nasonova Research Institute of Rheumatology started to form a Cost and Resource utilization Study in Antiretroviral treated patients (CoRSaR) cohort to investigate the evolution of nr-axSpA and treatment policy for this condition.  This communication deals with the preliminary results of a 12-month follow-up study of the CoRSaR  cohort.Subjects and methods. The cohort was recruited  from all consecutive patients with a &lt; 5-year history of nr-axSpA who sought for medical advice and treatment. The patients were examined in accordance  with the Ankylosing Spondylitis Assessments (ASAS) International Working Group  guidelines at inclusion and after 12 months.A total of 94 patients with axSpA were enrolled; 54 patients out of them were included into a preliminary analysis of the evolution of axSpA; their mean age at inclusion was 27.1±5.5  years and the mean disease duration  was 22.4±15.1 months;  49 (90.7%) patients were HLA-B27 positive. 31 of the 54 patients had ankylosing spondylitis(AS) and 23 had nr-axSpA.Results and discussion. The patients with AS and those with nr-axSpA showed virtually no difference in main clinical parameters,  such as the presence of arthritis, enthesitis, BASDAI, ASDAS-CRP, and BASFI. By the end of the 12month follow-up, both groups virtually displayed almost a double reduction  in all inflammatory markers but erythrocyte sedimentation rate. Nine (39%) patients with nr-axSpA developed radiographic sacroiliitis over 12 months and the diagnosis of AS was confirmed.Conclusion. 39% patients with nr-axSpA developed radiographic sacroiliitis at the 12-month follow-up. Nr-axSpA should be regarded as an early stage of AS

New approach to the approximation of «dose – effect» dependence during the human somatic cells irradiation

New data on cytogenetic approximation of the experimental cytogenetic dependence "dose - effect" based on the spline regression model that improves biological dosimetry of human radiological exposure were received. This is achieved by reducing the error of the determination of absorbed dose as compared to the traditional use of linear and linear-quadratic models and makes it possible to predict the effect of dose curves on plateau