AI-based structure prediction empowers integrative structural analysis of human nuclear pores

Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport. Their intricate 120-megadalton architecture remains incompletely understood. Here, we report a 70-megadalton model of the humanNPC scaffold with explicit membrane and in multiple conformational states. We combined artificial intelligence (AI)–based structure prediction with in situ and in cellulo cryo–electron tomography and integrative modeling. We show that linker nucleoporins spatially organize the scaffold within and across subcomplexes to establish the higher-order structure. Microsecond-long molecular dynamics simulationssuggest that the scaffold is not required to stabilize the inner and outer nuclear membrane fusion but rather widens the central pore. Our work exemplifies how AI-based modeling can be integrated within situ structural biology to understand subcellular architecture across spatial organization levels

Role of private foreign investment in the transfer of manufacturing technologies to developing countries : conclusions from a case study in Zaire

Meeting: Canadian Economics Association, Meeting, 1977, CAPhotocopyIDRC supported. Survey of the mechanisms, agents and results of technology transfers, and the role of private foreign investment in Zaire's industrial sector - describes patterns of technology transfer in a sample of 63 firms; includes a cost benefit analysis of technology transfers and recommends industrial policy promoting technical and managerial training by imposing contraints on foreign enterprises with respect to their training efforts

Technology transfer and the choice of industrial technologies in a developing country : the case of Zaire

ThesisThesis, University of Toronto, 197