100 research outputs found

    Hemodynamics in the thoracic aorta using OpenFOAM: 4D PCMRI versus CFD

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    The aim of this work is the study of fluid dynamics models using the CFD software OpenFOAM, an open source software allowing meshing, manipulation, simulation and post-processing of many problems involving fluid mechanics. The work consists of a study with OpenFOAM of a real engineering problem, namely to analyze hemodynamics in the thoracic aorta in collaboration with CIMNE (Centre Internacional de Metodes Numerics a l'Enginyeria) and LABSON-UPC (Laboratorio de Sistemas Oleohidricos y Neumcos). Speci cally, the study aims to compute the shear stress that blood causes to aorta walls

    Pepper pectin methylesterase inhibitor protein CaPMEI1 is required for antifungal activity, basal disease resistance and abiotic stress tolerance

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    Pectin is one of the main components of the plant cell wall that functions as the primary barrier against pathogens. Among the extracellular pectinolytic enzymes, pectin methylesterase (PME) demethylesterifies pectin, which is secreted into the cell wall in a highly methylesterified form. Here, we isolated and functionally characterized the pepper (Capsicum annuum L.) gene CaPMEI1, which encodes a pectin methylesterase inhibitor protein (PMEI), in pepper leaves infected by Xanthomonascampestris pv. vesicatoria (Xcv). CaPMEI1 transcripts are localized in the xylem of vascular bundles in leaf tissues, and pathogens and abiotic stresses can induce differential expression of this gene. Purified recombinant CaPMEI1 protein not only inhibits PME, but also exhibits antifungal activity against some plant pathogenic fungi. Virus-induced gene silencing of CaPMEI1 in pepper confers enhanced susceptibility to Xcv, accompanied by suppressed expression of some defense-related genes. Transgenic ArabidopsisCaPMEI1-overexpression lines exhibit enhanced resistance to Pseudomonas syringae pv. tomato, mannitol and methyl viologen, but not to the biotrophic pathogen Hyaloperonospora parasitica. Together, these results suggest that CaPMEI1, an antifungal protein, may be involved in basal disease resistance, as well as in drought and oxidative stress tolerance in plants
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