Structured Proposal for Rehabilitation Nursing (RN) Care Intervention: Sensitive Gains to RN Care for the Person with Self-care Deficit and in the Surgical Process

Surgical needs have been growing in Portugal due to the aging population, the increasing number of people with acute and chronic disease with consequent acquisition of deficiencies and restrictions, being verified dependence on self-care of the people in surgical process. The purpose of rehabilitation nursing intervention is to enable the person to perform his/her self-care and demonstrate the sensitive results to rehabilitation nursing care, allowing the quality of care provided to be proven. Objective. To evaluate the functionality of the person in the surgical process and the gains gained in the rehabilitation nursing care verified after the implementation of a structured proposal of intervention of nursing care of Rehabilitation. Methodology. The study is descriptive and exploratory, using the qualitative methodology of Robert Yin (multiple case studies), Lopes’ medium-range theory and the self-care model of Fonseca and Lopes, based on the nursing theory of the self-care of Orem. Results. There was a significant increase in the functionality of the person and the sensitive results to rehabilitation nursing care. Conclusion. The intervention of Rehabilitation Nursing through the application of a structured intervention plan, with the objective of enabling the person and caregiver to achieve maximum functionality and independence, translate into positive sensitive results to nursing care

Hipergranulação: Boas Práticas em Enfermagem

Objectivo: É fundamental os enfermeiros identificarem o tecido de hipergranulação e compreenderem o que pode traduzir em termos do ambiente biológico da ferida. É uma entidade comum nas feridas complexas, que se for gerida consegue-se controlar a cicatrização que se encontra estagnada. Com esta Revisão Sistemática da Literatura apoiada num estudo de caso, identificaram-se as intervenções de Enfermagem para a gestão de feridas complexas com tecido de hipergranulação. Metodologia: Foi efectuada uma pesquisa no motor de busca EBSCO, seleccionando bases de dados específicas e utilizados os descritores: “HYPERGRANULATION” or “OVERGRANULATION” or “HYPERTROPHIC GRANULATION” oy “HYPERPLASIA OF GRANULATION TISSUE” and “WOUND” and “ASSESSMENT”. Recorreu-se ao método PI©O e seleccionados um total de 13 artigos. Conclusão: Os cuidados de Enfermagem, o modus operandi, face às feridas com tecido de hipergranulação é ainda algo ambíguo e empirista. Com base nas evidências científicas existentes sobre a problemática definiram-se directrizes para a prática clínica

Interactomics and structure-function analysis of the RLTPR protein underpin its essential role for costimulation via CD28 in mouse and human T cells

International Congress of Immunology (ICI), Melbourne, AUSTRALIA, AUG 21-26, 2016International audienceno abstrac

Interactomics and structure-function analysis of the RLTPR protein underpin its essential role for costimulation via CD28 in mouse and human T cells

International Congress of Immunology (ICI), Melbourne, AUSTRALIA, AUG 21-26, 2016International audienceno abstrac

Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry

Spatiotemporal organization of protein interactions in cell signaling is a fundamental process that drives cellular functions. Given differential protein expression across tissues and developmental stages, the architecture and dynamics of signaling interaction proteomes is, likely, highly context dependent. However, current interaction information has been almost exclusively obtained from transformed cells. In this study, we applied an advanced and robust workflow combining mouse genetics and affinity purification (AP)-SWATH mass spectrometry to profile the dynamics of 53 high-confidence protein interactions in primary T cells, using the scaffold protein GRB2 as a model. The workflow also provided a sufficient level of robustness to pinpoint differential interaction dynamics between two similar, but functionally distinct, primary T cell populations. Altogether, we demonstrated that precise and reproducible quantitative measurements of protein interaction dynamics can be achieved in primary cells isolated from mammalian tissues, allowing resolution of the tissue-specific context of cell-signaling events